Results 1 to 10 of about 2,784 (175)

A type III-A CRISPR-Cas system employs degradosome nucleases to ensure robust immunity [PDF]

eLife, 2019
CRISPR-Cas systems provide sequence-specific immunity against phages and mobile genetic elements using CRISPR-associated nucleases guided by short CRISPR RNAs (crRNAs).
Lucy Chou-Zheng, Asma Hatoum-Aslan
doaj   +5 more sources

Membrane recognition and dynamics of the RNA degradosome.

PLoS Genetics, 2015
RNase E, which is the central component of the multienzyme RNA degradosome, serves as a scaffold for interaction with other enzymes involved in mRNA degradation including the DEAD-box RNA helicase RhlB.
Henrik Strahl, Catherine Turlan, Syma Khalid, Peter J Bond, Jean-Marie Kebalo, Pascale Peyron, Leonora Poljak, Marie Bouvier, Leendert Hamoen, Ben F Luisi, Agamemnon J Carpousis   +10 more
doaj   +7 more sources

The DEAD-box RNA helicase RhlB is required for efficient RNA processing at low temperature in Caulobacter [PDF]

Microbiology Spectrum, 2023
The RNA helicases from the DEAD-box family participate in several biochemical processes, with special relevance in the assembly of ribosome ribonucleoparticles and in RNA turnover as part of the multi-enzymatic RNA degradosome complex.
Hugo L. de Araújo, Beatriz A. Picinato, Alan P. R. Lorenzetti, Nisansala S. Muthunayake, I. W. Rathnayaka-Mudiyanselage, Naara M. dos Santos, Jared Schrader, Tie Koide, Marilis V. Marques   +8 more
doaj   +2 more sources

Polyribosome-Dependent Clustering of Membrane-Anchored RNA Degradosomes To Form Sites of mRNA Degradation in Escherichia coli

mBio, 2021
Here, we show that RNase E, RhlB, and PNPase act together as components of the multienzyme RNA degradosome in polyribosome-dependent clustering to form puncta on the inner cytoplasmic membrane.
Lina Hamouche, Leonora Poljak, Agamemnon J. Carpousis   +2 more
doaj   +2 more sources

Structure of the 30S translation initiation complex coupled to paused RNA polymerase and its potential for riboregulation [PDF]

Nature Communications
In many bacterial species, transcription and translation can be coupled physically, with potential impact on the rates and efficiency of gene expression. Here, we present structural evidence from cryo-EM demonstrating that a bacterial RNA polymerase that
Johann J. Roske, Giulia Paris, Akanksha Goyal, Marina Rodnina, Nikolay Zenkin, Katarzyna J. Bandyra, Ben F. Luisi   +6 more
doaj   +3 more sources

Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly [PDF]

Open Biology, 2012
Polynucleotide phosphorylase (PNPase) is an exoribonuclease that cleaves single-stranded RNA substrates with 3′–5′ directionality and processive behaviour.
Steven W. Hardwick, Tobias Gubbey, Isabelle Hug, Urs Jenal, Ben F. Luisi   +4 more
doaj   +2 more sources

Attachment of the RNA degradosome to the bacterial inner cytoplasmic membrane prevents wasteful degradation of rRNA in ribosome assembly intermediates.

PLoS Biology, 2023
RNA processing and degradation shape the transcriptome by generating stable molecules that are necessary for translation (rRNA and tRNA) and by facilitating the turnover of mRNA, which is necessary for the posttranscriptional control of gene expression ...
Lydia Hadjeras, Marie Bouvier, Isabelle Canal, Leonora Poljak, Quentin Morin-Ogier, Carine Froment, Odile Burlet-Schlitz, Lina Hamouche, Laurence Girbal, Muriel Cocaign-Bousquet, Agamemnon J Carpousis   +10 more
doaj   +3 more sources

Critical functions and key interactions mediated by the RNase E scaffolding domain in Pseudomonas aeruginosa. [PDF]

PLoS Genetics
The RNA degradosome is a bacterial multi-protein complex mediating mRNA processing and degradation. In Pseudomonadota, this complex assembles on the C-terminal domain (CTD) of RNase E through short linear motifs (SLiMs) that determine its composition and
Sandra Amandine Marie Geslain, Stéphane Hausmann, Johan Geiser, George Edward Allen, Diego Gonzalez, Martina Valentini   +5 more
doaj   +2 more sources

The ribonuclease E regulator RebA is essential for diazotrophic growth in the cyanobacterium <i>Anabaena</i> PCC 7120. [PDF]

mLife
Abstract Ribonuclease E (RNase E) is central to bacterial RNA metabolism. In cyanobacteria, its activity is inhibited by RebA, a key mechanism for controlling cell morphology. Here, we demonstrate that rebA is essential for diazotrophic growth of Anabaena PCC 7120, a filamentous cyanobacterium capable of forming heterocysts—specialized nitrogen‐fixing ...
Liu S, Wang Z, Lin G, Li W, Zeng X, Zhang JY, Zhang CC.   +6 more
europepmc   +2 more sources

The BR-body proteome contains a complex network of protein-protein and protein-RNA interactions [PDF]

Cell Reports, 2023
Summary: Bacterial ribonucleoprotein bodies (BR-bodies) are non-membrane-bound structures that facilitate mRNA decay by concentrating mRNA substrates with RNase E and the associated RNA degradosome machinery.
Vidhyadhar Nandana, Imalka W. Rathnayaka-Mudiyanselage, Nisansala S. Muthunayake, Ali Hatami, C. Bruce Mousseau, Luis A. Ortiz-Rodríguez, Jamuna Vaishnav, Michael Collins, Alisa Gega, Kaveendya S. Mallikaarachchi, Hadi Yassine, Aishwarya Ghosh, Julie S. Biteen, Yingxi Zhu, Matthew M. Champion, W. Seth Childers, Jared M. Schrader   +16 more
doaj   +2 more sources