Cooperation of regulatory RNA and the RNA degradosome in transcript surveillance. [PDF]
Nucleic Acids ResThe ompD transcript, encoding an outer membrane porin in Salmonella, harbors a controlling element in its coding region that base-pairs imperfectly with a ‘seed’ region of the small regulatory RNA (sRNA) MicC.
Bandyra KJ +5 more
europepmc +12 more sources
The RNase J-Based RNA Degradosome Is Compartmentalized in the Gastric Pathogen
mBio, 2020 Posttranscriptional regulation is a major level of gene expression control in any cell. In bacteria, multiprotein machines called RNA degradosomes are central for RNA processing and degradation, and some were reported to be compartmentalized inside these
Alejandro Tejada-Arranz +5 more
doaj +8 more sources
Beyond a platform protein for the degradosome assembly: The Apoptosis-Inducing Factor as an efficient nuclease involved in chromatinolysis. [PDF]
PNAS Nexus, 2023 The Apoptosis-Inducing Factor (AIF) is a moonlighting flavoenzyme involved in the assembly of mitochondrial respiratory complexes in healthy cells, but also able to trigger DNA cleavage and parthanatos.
Novo N +10 more
europepmc +7 more sources
Membrane recognition and dynamics of the RNA degradosome.
PLoS Genetics, 2015 RNase E, which is the central component of the multienzyme RNA degradosome, serves as a scaffold for interaction with other enzymes involved in mRNA degradation including the DEAD-box RNA helicase RhlB.
Henrik Strahl +10 more
doaj +9 more sources
When translation elongation is impaired, the mRNA is uniformly destabilized by the RNA degradosome, while the concentration of mRNA is altered along the molecule. [PDF]
Nucleic Acids Res, 2023 mRNA sits at the crossroads of transcription, translation and mRNA degradation. Many questions remain about the coupling of these three processes in Escherichia coli and, in particular, how translation may have an effect on mRNA degradation and ...
Duviau MP +5 more
europepmc +4 more sources
Dimeric assembly of human Suv3 helicase promotes its
Protein Science, Volume 31, Issue 5, May 2022., 2022 Human Suv3 is a unique homodimeric helicase that constitutes the major component of the mitochondrial degradosome to work cooperatively with exoribonuclease PNPase for efficient RNA decay.
Monika Jain +6 more
semanticscholar +3 more sources
Attachment of the RNA degradosome to the bacterial inner cytoplasmic membrane prevents wasteful degradation of rRNA in ribosome assembly intermediates. [PDF]
PLoS Biology, 2023 RNA processing and degradation shape the transcriptome by generating stable molecules that are necessary for translation (rRNA and tRNA) and by facilitating the turnover of mRNA, which is necessary for the posttranscriptional control of gene expression ...
Lydia Hadjeras +10 more
doaj +3 more sources
Structural analysis of mtEXO mitochondrial RNA degradosome reveals tight coupling of nuclease and helicase components [PDF]
Nature Communications, 2018 The mitochondrial RNA degradosome (mtEXO) plays an essential role in the regulation of mitochondrial gene expression and is composed of the 3′-to-5′ exoribonuclease Dss1 and the helicase Suv3.
Michal Razew +12 more
doaj +3 more sources
Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly [PDF]
Open Biology, 2012 Polynucleotide phosphorylase (PNPase) is an exoribonuclease that cleaves single-stranded RNA substrates with 3′–5′ directionality and processive behaviour.
Steven W. Hardwick +4 more
doaj +3 more sources
The DEAD-box RNA helicase RhlB is required for efficient RNA processing at low temperature in Caulobacter [PDF]
Microbiology Spectrum, 2023 The RNA helicases from the DEAD-box family participate in several biochemical processes, with special relevance in the assembly of ribosome ribonucleoparticles and in RNA turnover as part of the multi-enzymatic RNA degradosome complex.
Hugo L. de Araújo +8 more
doaj +2 more sources