Results 21 to 30 of about 2,784 (175)

A minimal bacterial RNase J-based degradosome is associated with translating ribosomes [PDF]

Nucleic Acids Research, 2012
Protein complexes directing messenger RNA (mRNA) degradation are present in all kingdoms of life. In Escherichia coli, mRNA degradation is performed by an RNA degradosome organized by the major ribonuclease RNase E. In bacteria lacking RNase E, the existence of a functional RNA degradosome is still an open question.
Yulia Redko, Sylvie Aubert, Anna Stachowicz, Pascal Lenormand, Abdelkader Namane, Fabien Darfeuille, Marie Thibonnier, Hilde De Reuse   +7 more
openalex   +3 more sources

TheYersinia pseudotuberculosisdegradosome is required for oxidative stress, while its PNPase subunit plays a degradosome-independent role in cold growth [PDF]

FEMS Microbiology Letters, 2012
Yersinia polynucleotide phosphorylase (PNPase), a 3'-5' exoribonuclease, has been shown to affect growth during several stress responses. In Escherichia coli, PNPase is one of the subunits of a multiprotein complex known as the degradosome, but also has degradosome-independent functions. The carboxy-terminus of E.
Amanda Henry, Justin Shanks, Ambro van Hoof, Jason A. Rosenzweig   +3 more
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Moonlighting in Bacillus subtilis: The Small Proteins SR1P and SR7P Regulate the Moonlighting Activity of Glyceraldehyde 3-Phosphate Dehydrogenase A (GapA) and Enolase in RNA Degradation

Microorganisms, 2021
Moonlighting proteins are proteins with more than one function. During the past 25 years, they have been found to be rather widespread in bacteria. In Bacillus subtilis, moonlighting has been disclosed to occur via DNA, protein or RNA binding or protein ...
Inam Ul Haq, Sabine Brantl
doaj   +1 more source

When translation elongation is impaired, the mRNA is uniformly destabilized by the RNA degradosome, while the concentration of mRNA is altered along the molecule. [PDF]

Nucleic Acids Res, 2023
Duviau MP, Chen F, Emile A, Cocaign-Bousquet M, Girbal L, Nouaille S.   +5 more
europepmc   +3 more sources

Recognition of the 70S ribosome and polysome by the RNA degradosome in Escherichia coli [PDF]

Nucleic Acids Research, 2012
The RNA degradosome is a multi-enzyme assembly that contributes to key processes of RNA metabolism, and it engages numerous partners in serving its varied functional roles. Small domains within the assembly recognize collectively a diverse range of macromolecules, including the core protein components, the cytoplasmic lipid membrane, mRNAs, non-coding ...
Yi‐Chun Isabella Tsai, Dijun Du, Lilianha Domínguez-Malfavón, Daniela Dimastrogiovanni, Jonathan Cross, Anastasia J. Callaghan, Jaime García‐Mena, Ben F. Luisi   +7 more
openalex   +5 more sources

Cooperation of regulatory RNA and the RNA degradosome in transcript surveillance. [PDF]

Nucleic Acids Res
Abstract The ompD transcript, encoding an outer membrane porin in Salmonella, harbors a controlling element in its coding region that base-pairs imperfectly with a ‘seed’ region of the small regulatory RNA (sRNA) MicC. When tagged with the sRNA, the ompD mRNA is cleaved downstream of the pairing site by the conserved endoribonuclease ...
Bandyra KJ, Fröhlich KS, Vogel J, Rodnina M, Goyal A, Luisi BF.   +5 more
europepmc   +9 more sources

The Structure and Function of the Gram-Positive Bacterial RNA Degradosome [PDF]

Frontiers in Microbiology, 2017
The RNA degradosome is a highly structured protein complex responsible for bulk RNA decay in bacteria. The main components of the complex, ribonucleases, an RNA helicase, and glycolytic enzymes are well-conserved in bacteria. Some components of the degradosome are essential for growth and the disruption of degradosome formation causes slower growth ...
Kyu Hong Cho
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The RNase J-Based RNA Degradosome Is Compartmentalized in the Gastric Pathogen Helicobacter pylori

mBio, 2020
Posttranscriptional regulation is a major level of gene expression control in any cell. In bacteria, multiprotein machines called RNA degradosomes are central for RNA processing and degradation, and some were reported to be compartmentalized inside these
Alejandro Tejada-Arranz, Eloïse Galtier, Lamya El Mortaji, Evelyne Turlin, Dmitry Ershov, Hilde De Reuse   +5 more
doaj   +1 more source

Analysis of mRNA Decay Intermediates in Bacillus subtilis 3′ Exoribonuclease and RNA Helicase Mutant Strains

mBio, 2022
The Bacillus subtilis genome encodes four 3′ exoribonucleases: polynucleotide phosphorylase (PNPase), RNase R, RNase PH, and YhaM. Previous work showed that PNPase, encoded by the pnpA gene, is the major 3′ exonuclease involved in mRNA turnover; in a ...
Shivani Chhabra, Zachary F. Mandell, Bo Liu, Paul Babitzke, David H. Bechhofer   +4 more
doaj   +1 more source

Global analysis of Escherichia coli RNA degradosome function using DNA microarrays [PDF]

Proceedings of the National Academy of Sciences, 2004
RNase E, an essential endoribonuclease of Escherichia coli , interacts through its C-terminal region with multiple other proteins to form a complex termed the RNA degradosome. To investigate the degradosome's proposed role as an RNA decay machine, we used DNA microarrays to globally assess alterations in the steady ...
Jonathan A. Bernstein, Pei-Hsun Lin, Stanley N. Cohen, Sue Lin‐Chao   +3 more
openalex   +3 more sources