Results 41 to 50 of about 3,511 (227)
Molecular recognition of RhlB and RNase D in the Caulobacter crescentus RNA degradosome [PDF]
Nucleic Acids Research, 2014 The endoribonuclease RNase E is a key enzyme in RNA metabolism for many bacterial species. In Escherichia coli, RNase E contributes to the majority of RNA turnover and processing events, and the enzyme has been extensively characterized as the central ...
Jarrod E. Voss, B. Luisi, S. Hardwick
semanticscholar +5 more sources
The Escherichia coli major exoribonuclease RNase II is a component of the RNA degradosome [PDF]
Bioscience Reports, 2014 Multiprotein complexes that carry out RNA degradation and processing functions are found in cells from all domains of life. In Escherichia coli, the RNA degradosome, a four-protein complex, is required for normal RNA degradation and processing.
Feng Lu, A. Taghbalout
semanticscholar +3 more sources
Polyphosphate kinase is a component of the Escherichia coli RNA degradosome [PDF]
Molecular Microbiology, 1997 The Escherichia coli degradosome is a multienzyme complex with four major protein components: the endoribonuclease RNase E, the exoribonuclease PNPase, the RNA helicase RhlB and enolase. The first three of these proteins are known to have important functions in mRNA processing and degradation.
Erwin Blum +3 more
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Inflammatory mitochondrial nucleic acids as drivers of pathophysiology. [PDF]
Clin Transl Med, 2023 Clinical and Translational Medicine, Volume 13, Issue 9, September 2023.
Peace CG, Hooftman A, Ryan DG.
europepmc +2 more sources
Bacterial RNA Degradosomes: Molecular Machines under Tight Control [PDF]
Trends in Biochemical Sciences, 2019 Bacterial RNA degradosomes are multienzyme molecular machines that act as hubs for post-transcriptional regulation of gene expression. The ribonuclease activities of these complexes require tight regulation, as they are usually essential for cell survival while potentially destructive.
Alejandro Tejada-Arranz +2 more
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Protein Pulldown Assays to Monitor the Composition of the Bacterial RNA Degradosome.
Methods in molecular biology, 2021 The method of co-immunoprecipitation (co-IP or pulldown) enables the identification of proteins interacting in macromolecular assemblies, through the purification of a key protein by affinity chromatography using specific antibodies immobilized on a matrix.
S. Hardwick, B. Luisi, M. Marques
semanticscholar +4 more sources
Crystal structure of the Escherichia coli RNA degradosome component enolase [PDF]
Journal of Molecular Biology, 2001 The crystal structure of Escherichia coli enolase (EC 4.2.1.11, phosphopyruvate hydratase), which is a component of the RNA degradosome, has been determined at 2.5 A. There are four molecules in the asymmetric unit of the C2 cell, and in one of the molecules, flexible loops close onto the active site.
Karin Kühnel, Ben F. Luisi
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Yeast mitochondrial RNase P, RNase Z and the RNA degradosome are part of a stable supercomplex [PDF]
Nucleic Acids Research, 2011 Initial steps in the synthesis of functional tRNAs require 5′- and 3′-processing of precursor tRNAs (pre-tRNAs), which in yeast mitochondria are achieved by two endonucleases, RNase P and RNase Z. In this study, using a combination of detergent-free Blue
Rachid Daoud, L. Forget, B. F. Lang
semanticscholar +4 more sources
mBio, 2021 Here, we show that RNase E, RhlB, and PNPase act together as components of the multienzyme RNA degradosome in polyribosome-dependent clustering to form puncta on the inner cytoplasmic membrane.
Lina Hamouche +2 more
doaj +1 more source