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Analysis of kimchi microflora using denaturing gradient gel electrophoresis

International Journal of Food Microbiology, 2005
A polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique was used to determine the microfloral composition during the fermentation of kimchi, a traditional Korean fermented vegetable food. The kimchi was fermented at 10 degrees C or 20 degrees C for 30 or 20 days, respectively.
Jung-Sook Lee, Yu-Ryang Pyun, Jong Seog Ahn   +2 more
exaly   +4 more sources

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Constant denaturant gel electrophoresis, a modification of denaturing gradient gel electrophoresis, in mutation detection

Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 1991
Denaturing gradient gel electrophoresis (DGGE) is increasingly being utilized in mutational detection, both in characterization of variations in genomic DNA and in the generation of mutational spectra after in vitro and in vivo mutagenesis. The basis for this electrophoretic separation technique is strand dissociation of DNA fragments in discrete ...
Eivind Hovig, Birgitte Smith-Sørensen, A Brøgger   +2 more
exaly   +5 more sources

Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology

Antonie van Leeuwenhoek, 1998
Here, the state of the art of the application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology will be presented. Furthermore, the potentials and limitations of these techniques will be discussed, and it will be indicated why their use in ecological studies has become so ...
Muyzer, G., Smalla, K.
openaire   +4 more sources

The sensitivity of denaturing gradient gel electrophoresis: a blinded analysis

Mutation Research - Mutation Research Genomics, 1998
Denaturing gradient gel electrophoresis (DGGE) is considered one of the most sensitive and specific of the mutational scanning techniques, yet blinded analyses have not been reported. We report the results of a blinded study of the efficiency of DGGE to detect mutations in the Human Coagulation Factor IX. Two overlapping genomic DNA sequences from exon
Pablo V Gejman, Steve Sommer
exaly   +3 more sources

Mutational analysis using denaturing gradient gel electrophoresis and PCR

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1993
Denaturing gradient gel electrophoresis (DGGE) separates (DNA) molecules based on their sequence. Using the proper conditions, all base-pair substitutions can be resolved from the wild-type sequence using DGGE. Polymerase chain reaction (PCR) permits rapid amplification of a given region of the genome.
Neal F Cariello, Thomas R Škopek
exaly   +3 more sources

Detection of Mutations by Denaturing Gradient Gel Electrophoresis

Current Protocols in Human Genetics, 1998
AbstractThis unit describes the procedure for determining the melting profile for a given PCR‐amplified sequence by perpendicular denaturing gradient gel electrophoresis (DGGE) and, using that information, for developing a screening assay based on either parallel DGGE, CDGE (Constant Denaturant Gel Electrophoresis), or TTGE (Temporal /Temperature ...
A L, Børresen-Dale, E, Hovig, B, Smith-Sørensen   +2 more
openaire   +2 more sources

Denaturing Gradient Gel Electrophoresis (DGGE)

2013
Denaturing Gradient Gel Electrophoresis (DGGE) is a technique used to separate short- to medium-length DNA fragments based on their melting characteristics. It has been used frequently for identifying single-nucleotide polymorphisms without the need for DNA sequencing and as a molecular fingerprinting method for complex ecosystem communities, in ...
Fiona, Strathdee, Andrew, Free
openaire   +2 more sources

Discrimination of Cryptosporidium species by denaturing gradient gel electrophoresis

Parasitology Research, 2007
Denaturing gradient gel electrophoresis (DGGE) was used for the discrimination of three species and one genotype of the protozoan parasite Cryptosporidium: the C. parvum, C. andersoni, C. muris, and C. muris Japanese field mouse genotype. A set of primers specific for the 18S rRNA gene of Cryptosporidium was used in the DGGE; consequently, the four ...
Masaaki, Satoh, Yutaka, Nakai
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Mutation detection by denaturing gradient gel electrophoresis (DGGE)

Human Mutation, 1994
The molecular analysis of genetic diseases relies on several technical approaches which allow genetic and physical mapping, characterization of the gene structure, expression studies, and identification of disease-causing mutations. Denaturing gradient gel electrophoresis (DGGE) allows the rapid screening for single base changes in enzymatically ...
R, Fodde, M, Losekoot
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The gel edge electric field gradients in denaturing polyacrylamide gel electrophoresis

ELECTROPHORESIS, 1998
AbstractIt has previously been shown that zones of higher electric field form close to the loading end of the gel during denaturing polyacrylamide gel electrophoresis. Here we show that the field can reach up to three times its normal mean value a few cm in front of the loading wells when 44.5 mM Tris‐44.5 mM boric acid‐1 mM EDTA is used as the gel ...
C, Desruisseaux, G W, Slater, G, Drouin
openaire   +2 more sources