Results 251 to 260 of about 39,830 (289)

Rapid Detection of Avian Eimeria Species Using Denaturing Gradient Gel Electrophoresis

Poultry Science, 2008
A denaturing gradient gel electrophoresis (DGGE) assay was developed to rapidly discriminate species of avian Eimeria. Amplification by PCR of the small subunit ribosomal RNA gene (approximately 1,600 nucleotides) with Eimeria genus-specific primers ...
Syvyk, A., Hume, M. E., Teplova, I., Nalian, A., Martynova-Van Kley, Alexandra   +4 more
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Analysis of mutations using PCR and denaturing gradient gel electrophoresis

Environmental and Molecular Mutagenesis, 1991
AbstractDenaturing gradient gel electrophoresis (DGGE) separates DNA molecules based on primary sequence. Under the appropriate conditions, all base pair (bp) substitutions, frame‐shifts, and deletions less than about 10 bp can be resolved from the wild type sequence using DGGE.
N F, Cariello, J A, Swenberg, A, De Bellis, T R, Skopek   +3 more
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Denaturing Gradient Gel Electrophoresis

1999
Denaturing gradient gel electrophoresis (DGGE) (Abrams and Stanton 1992; Myers et al. 1987) is a technique suitable for detecting point mutations as well as small deletions and insertions of several base pairs in double stranded DNA. The method is based on the analysis of strand separation (denaturation) of DNA molecules.
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Polymorphisms of human TAP2 detected by denaturing gradient gel electrophoresis

Human Immunology, 2003
The human transporter associated with antigen processing (TAP1 and TAP2) genes are located in the human leukocyte antigen (HLA) class II region of the genome and encode proteins that form a heterodimer essential for the transport of endogenous peptides into the endoplasmic reticulum for assembly with HLA class I molecules.
Alfred, Penfornis, Gang, Yan, Lijia, Shi, Denise L, Faustman   +3 more
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Human TAP1 polymorphisms detected by denaturing gradient gel electrophoresis

Tissue Antigens, 1997
Presentation of endogenous peptides by major histocompatibility complex class I (MHC) molecules is controlled, in part, by the Tap1 and Tap2 genes in the MHC class II region that encode a heterodimeric peptide transporter. Polymorphisms of human Tap1 in normal individuals have now been investigated systematically by denaturing gradient gel ...
L, Shi, G, Yan, Y, Fu, L, Ma, A, Penfornis, D, Faustman   +5 more
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Detection of mutations in insulin receptor gene by denaturing gradient gel electrophoresis

Diabetes, 1992
Denaturing gradient gel electrophoresis (DGGE) has been used to screen for mutations in the insulin receptor gene. Each of the 22 exons was amplified by the polymerase chain reaction (PCR). For each exon, one of the two PCR primers contained a guanine-cytosine (GC) clamp at its 5′ end.
BARBETTI F., GEJMAN P. V., TAYLOR S. I., RABEN N., CAMA A., BONORA, Enzo, PIZZO P., MOGHETTI, Paolo, MUGGEO, Michele, ROTH J.   +9 more
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Detection of p53 point mutations by double‐gradient, denaturing gradient gel electrophoresis

ELECTROPHORESIS, 1997
AbstractGenetic instability is a typical feature of tumor cells. This evidence has stimulated the development of rapid methods for detection of gene mutations. A new, improved protocol for denaturing gradient gel electrophoresis (DGGE), to screen for point mutations in genomic DNA, is reported: double gradient (DG) DGGE.
C, Gelfi, S C, Righetti, F, Zunino, G, Della Torre, M A, Pierotti, P G, Righetti   +5 more
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[Application of denaturing gradient gel electrophoresis and temperature gradient gel electrophoresis in microbial molecular ecology].

Wei sheng wu xue bao = Acta microbiologica Sinica, 2009
In recent years, denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) are well-established molecular tools in microbial molecular ecology that allows the study of diversity and dynamics of microbial communities. The technique is reliable, reproducible rapid and inexpensive.
Man-Li, Gong, Nan-Qi, Ren, De-Feng, Xing
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Single Nucleotide Polymorphism Screening with Denaturing Gradient Gel Electrophoresis

2009
Denaturing gradient gel electrophoresis (DGGE) is a powerful technique for identifying DNA sequence-based differences. The method relies on the fact that double-stranded DNA molecules have unique denaturation rates that are based upon the specific nucleotide composition of the DNA sequence(s).
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Denaturing Gradient Gel Electrophoresis to Detect Methylation Changes in DNA

2004
Denaturing gradient gel electrophoresis (DGGE) is a technique that fractionates DNA molecules on the basis of their melting behavior and thereby permits the separation of DNA fragments with local variations in base composition. The separation of DNA fragments by DGGE is determined by the nucleotide sequence, rather than size. This approach is effective
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