Results 251 to 260 of about 78,856 (294)

Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology

Antonie van Leeuwenhoek, 1998
Here, the state of the art of the application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology will be presented. Furthermore, the potentials and limitations of these techniques will be discussed, and it will be indicated why their use in ecological studies has become so ...
Muyzer, G., Smalla, K.
openaire   +3 more sources

The gel edge electric field gradients in denaturing polyacrylamide gel electrophoresis

ELECTROPHORESIS, 1998
AbstractIt has previously been shown that zones of higher electric field form close to the loading end of the gel during denaturing polyacrylamide gel electrophoresis. Here we show that the field can reach up to three times its normal mean value a few cm in front of the loading wells when 44.5 mM Tris‐44.5 mM boric acid‐1 mM EDTA is used as the gel ...
C, Desruisseaux, G W, Slater, G, Drouin
openaire   +2 more sources

Denaturing Gradient Gel Electrophoresis

1999
Denaturing gradient gel electrophoresis (DGGE) (Abrams and Stanton 1992; Myers et al. 1987) is a technique suitable for detecting point mutations as well as small deletions and insertions of several base pairs in double stranded DNA. The method is based on the analysis of strand separation (denaturation) of DNA molecules.
openaire   +1 more source

Polymorphisms of human TAP2 detected by denaturing gradient gel electrophoresis

Human Immunology, 2003
The human transporter associated with antigen processing (TAP1 and TAP2) genes are located in the human leukocyte antigen (HLA) class II region of the genome and encode proteins that form a heterodimer essential for the transport of endogenous peptides into the endoplasmic reticulum for assembly with HLA class I molecules.
Alfred, Penfornis, Gang, Yan, Lijia, Shi, Denise L, Faustman   +3 more
openaire   +2 more sources

The sensitivity of denaturing gradient gel electrophoresis: a blinded analysis

Mutation Research/Mutation Research Genomics, 1998
Denaturing gradient gel electrophoresis (DGGE) is considered one of the most sensitive and specific of the mutational scanning techniques, yet blinded analyses have not been reported. We report the results of a blinded study of the efficiency of DGGE to detect mutations in the Human Coagulation Factor IX. Two overlapping genomic DNA sequences from exon
P V, Gejman, Q, Cao, F, Guedj, S, Sommer
openaire   +2 more sources

Analysis of mutations using PCR and denaturing gradient gel electrophoresis

Environmental and Molecular Mutagenesis, 1991
AbstractDenaturing gradient gel electrophoresis (DGGE) separates DNA molecules based on primary sequence. Under the appropriate conditions, all base pair (bp) substitutions, frame‐shifts, and deletions less than about 10 bp can be resolved from the wild type sequence using DGGE.
N F, Cariello, J A, Swenberg, A, De Bellis, T R, Skopek   +3 more
openaire   +2 more sources

Single Nucleotide Polymorphism Screening with Denaturing Gradient Gel Electrophoresis

2009
Denaturing gradient gel electrophoresis (DGGE) is a powerful technique for identifying DNA sequence-based differences. The method relies on the fact that double-stranded DNA molecules have unique denaturation rates that are based upon the specific nucleotide composition of the DNA sequence(s).
openaire   +2 more sources

Detection of p53 point mutations by double‐gradient, denaturing gradient gel electrophoresis

ELECTROPHORESIS, 1997
AbstractGenetic instability is a typical feature of tumor cells. This evidence has stimulated the development of rapid methods for detection of gene mutations. A new, improved protocol for denaturing gradient gel electrophoresis (DGGE), to screen for point mutations in genomic DNA, is reported: double gradient (DG) DGGE.
C, Gelfi, S C, Righetti, F, Zunino, G, Della Torre, M A, Pierotti, P G, Righetti   +5 more
openaire   +2 more sources

Comprehensive mutation detection with denaturing gradient gel electrophoresis

1998
Abstract Since the basic laboratory procedures for denaturing gradient electrophoresis and the introduction of G,C clamps have not changed significantly from previous descriptions, and they depart only slightly from conventional gel electrophoresis, we provide guidance and references to published complete instructions.
L S Lerman, Cherif Beldjord
openaire   +1 more source

Denaturing gradient gel electrophoresis in marine microbial ecology

2001
Publisher Summary Genetic fingerprinting techniques are excellently suited to comparison of large numbers of samples. Genetic fingerprinting of microbial communities provides banding patterns or profiles that reflect the genetic diversity of the community.
Hendrik Schäfer, Gerard Muyzer
openaire   +1 more source