Results 11 to 20 of about 39,457 (305)

Replacement of 2′-Deoxycytidine by 2′-Deoxycytidine Analogues in the E. coli Genome [PDF]

Journal of the American Chemical Society, 2016
Several modified bases have been observed in the genomic DNA of bacteriophages, prokaryotes, and eukaryotes that play a role in restriction systems and/or epigenetic regulation. In our efforts to understand the consequences of replacing a large fraction of a canonical nucleoside with a modified nucleoside, we previously replaced around 75% of thymidine
Angad P, Mehta, Han, Li, Sean A, Reed, Lubica, Supekova, Tsotne, Javahishvili, Peter G, Schultz   +5 more
openaire   +3 more sources

THE NMR CONFORMATION STUDY OF THE COMPLEXES OF DEOXYCYTIDINE KINASE (dCK) AND 2′-DEOXYCYTIDINE/2′-DEOXYADENOSINE

Nucleosides, Nucleotides and Nucleic Acids, 2001
The structures of the bound 13C/2H double-labelled 2'(R/S), 5'(R/S)-2H2-1',2',3',4',5'-13C5-2'-deoxyadenosine and the corresponding 2'-deoxycytidine moieties in the complexes with human deoxycytidine kinase (dCK) have been characterized for the first time by the solution NMR spectroscopy, using Transferred Dipole-Dipole Cross-correlated Relaxation and ...
T, Maltseva, E, Usova, S, Eriksson, J, Milecki, A, Földesi, J, Chattopadhayaya   +5 more
openaire   +3 more sources

Purification and Properties of Human Deoxycytidine Kinase

, 1986
The purification of deoxycytidine kinase from human leucemic spleen reported here result in a pure protein of molecular weight 28K. The enzyme eluates during gel filtration as a dimer and the same enzyme phosphorylates both deoxycytidine, deoxyguanosine and deoxyadenosine, but with different Km and Vmax values. Our results are in agreement with earlier
C, Bohman, S, Eriksson
openaire   +3 more sources

Unified Description of Ultrafast Excited State Decay Processes in Epigenetic Deoxycytidine Derivatives [PDF]

, 2021
Epigenetic DNA modifications play a fundamental role in modulating gene expression and regulating cellular and developmental biological processes, thereby forming a second layer of information in DNA. The epigenetic 2′-deoxycytidine modification 5-methyl-
Marco Romanelli (7991153), Marco Garavelli (1308564), Marco Garavelli, Piotr Kabaciński, Eveliina Ponkkonen (11662824), Thomas Carell (199465), Irene Conti, Piotr Kabaciński (11662821), Marco Romanelli, Vishal Kumar Jaiswal (11662827), Thomas Carell, Vishal Kumar Jaiswal, Giulio Cerullo (118912), Irene Conti (2329084), Giulio Cerullo, Eveliina Ponkkonen   +15 more
core   +2 more sources

SPRTN and TDP1/TDP2 Independently Suppress 5‑Aza-2′-deoxycytidine-Induced Genomic Instability in Human TK6 Cell Line

, 2022
DNA-protein cross-links (DPCs) are generated by internal factors such as cellular aldehydes that are generated during normal metabolism and external factors such as environmental mutagens.
Hiroyuki Sasanuma (25521), Tadayoshi Bessho (356680), Keizo Tano (203831), Misa Miyakawa (14008505), Kouji Hirota (213716), Masanobu Kawanishi (3088098), Toshiaki Nakano (27092), Yuto Hanaichi (14008508), Masataka Tsuda (2808286), Takahito Moriwaki (3603782), Hiroshi Ide (27101)   +10 more
core   +1 more source

Gene reactivation by 5-aza-2'-deoxycytidine-induced demethylation requires SRCAP-mediated H2A.Z insertion to establish nucleosome depleted regions. [PDF]

PLoS Genetics, 2012
5-Aza-2'-deoxycytidine, approved by the FDA for the treatment of myelodysplastic syndrome (MDS), is incorporated into the DNA of dividing cells where it specifically inhibits DNA methylation by forming covalent complexes with the DNA methyltransferases ...
Xiaojing Yang, Houtan Noushmehr, Han Han, Claudia Andreu-Vieyra, Gangning Liang, Peter A Jones   +5 more
doaj   +1 more source

Pancreatic cancer resistance conferred by stellate cells: looking for new preclinical models

Experimental Hematology & Oncology, 2020
Pancreatic ductal adenocarcinoma (PDAC) has an extremely poor response to chemo- and (modest-dose conventionally fractionated) radio-therapy. Emerging evidence suggests that pancreatic stellate cells (PSCs) secrete deoxycytidine, which confers resistance
Pei Pei Che, Alessandro Gregori, Omidreza Firuzi, Max Dahele, Peter Sminia, Godefridus J. Peters, Elisa Giovannetti   +6 more
doaj   +1 more source

The effect of chemotherapeutic agents on telomere length maintenance in breast cancer cell lines [PDF]

, 2014
Copyright @ 2014 the authors. This article is made available through the Brunel Open Access Publishing Fund. It is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution ...
Slijepcevic, P, Terry Roberts, Yasaei, H, Roberts, T, Azadeh Motevalli, Motevalli, A, Predrag Slijepcevic, Sara Anjomani Virmouni, Hemad Yasaei, Virmouni, SA   +9 more
core   +1 more source

The Intracellular Localization of Deoxycytidine Kinase [PDF]

Journal of Biological Chemistry, 1998
Deoxycytidine kinase (dCK) catalyzes the rate-limiting step of the deoxynucleoside salvage pathway in mammalian cells and plays a key role in the activation of several pharmacologically important nucleoside analogs. Using a highly specific polyclonal antibody raised against a C-terminal peptide of the human dCK, we analyzed its subcellular localization
P, Hatzis, A S, Al-Madhoon, M, Jüllig, T G, Petrakis, S, Eriksson, I, Talianidis   +5 more
openaire   +2 more sources

Tissue-Specific Differences in DNA Modifications (5-Hydroxymethylcytosine, 5-Formylcytosine, 5-Carboxylcytosine and 5-Hydroxymethyluracil) and Their Interrelationships.

PLoS ONE, 2015
BackgroundReplication-independent active/enzymatic demethylation may be an important process in the functioning of somatic cells. The most plausible mechanisms of active 5-methylcytosine demethylation, leading to activation of previously silenced genes ...
Daniel Gackowski, Ewelina Zarakowska, Marta Starczak, Martyna Modrzejewska, Ryszard Olinski   +4 more
doaj   +1 more source