Results 11 to 20 of about 18,287 (222)

Development of a DGGE method to explore Legionella communities [PDF]

Heliyon, 2020
Legionella risk assessment is nowadays based on the presence and concentration of either Legionella pneumophila or Legionella spp. Many species of Legionella can cause Legionnaires’ disease, indeed about half of the known species have been associated ...
S. Bayle, B. Martinez-Arribas, S. Jarraud, P. Giannoni, L. Garrelly, B. Roig, A. Cadière   +6 more
doaj   +6 more sources

Double-Gradient DGGE for Optimized Detection of DNA Point Mutations [PDF]

BioTechniques, 1997
A novel technique is reported for screening point mutations in genomic DNA: double gradient, denaturing gradient gel electrophoresis (DG-DGGE). Unlike conventional DGGE, which exploits a single gradient of denaturing chemicals (typically urea and ...
L. Cremonesi, S. Firpo, M. Ferrari, P.G. Righetti, C. Gelfi   +4 more
doaj   +4 more sources

Comparison of PCR-DGGE and Nested-PCR-DGGE Approach for Ammonia Oxidizers Monitoring in Membrane Bioreactors’ Activated Sludge

Archives of Environmental Protection, 2014
Nitritation, the first stage of ammonia removal process is known to be limiting for total process performance. Ammonia oxidizing bacteria (AOB) which perform this process are obligatory activated sludge habitants, a mixture consisting of Bacteria ...
Ziembińska-Buczyńska Aleksandra, Wiszniowski Jarosław, Ciesielski Sławomir   +2 more
doaj   +3 more sources

Optimization of the DGGE band identification method

Folia Microbiologica, 2012
Denaturant gradient gel electrophoresis (DGGE) enables insight into the diversity of the studied microbial communities on the basis of separation of PCR amplification products according to their nucleotide sequence composition. However, the success of the method is accompanied by the inherent appearance of various sequence artifacts that bias the ...
Kušar, Darja, Avguštin, Gorazd
openaire   +4 more sources

Exon Concatenation to Increase the Efficiency of Mutation Screening by DGGE [PDF]

BioTechniques, 2002
For genes that have a substantial number of exons and long intronic sequences, mutation screening by denaturing gradient gel electrophoresis (DGGE) requires the amplification of each exon from genomic DNA by PCR.
X. Agirre, M. Garcí-Delgado, M.J. Calasanz, M.J. Larráyoz, F.J. Novo, J.L. Vizmanos   +5 more
doaj   +3 more sources

16S rRNA Targeted DGGE Fingerprinting of Microbial Communities

, 2008
The past decades have seen the staggering development of molecular microbial ecology as a discipline that uses the detection of so-called biomarkers to monitor microbial communities in environment samples. A variety of molecules can be used as biomarkers, including cell-wall components, proteins, lipids, DNA or RNA. Especially, the application of small
Tzeneva, V.A., Heilig, G.H.J., van Vliet, W.M., Akkermans, A.D.L., de Vos, W.M., Smidt, H.   +5 more
openaire   +4 more sources

Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives. [PDF]

Microb Biotechnol
Bacterial and archaeal communities power plant‐microbial fuel cells. This review integrates current knowledge through comprehensive tables and knowledge graphs, emphasising microbial diversity, functions, and biotechnological potential for renewable energy and environmental applications.
Freire JB, Faustino L, Abreu AA, Cruz IAGDE, Costa EJX.   +4 more
europepmc   +2 more sources

Assessment of ciliates in the sheep rumen by DGGE [PDF]

Letters in Applied Microbiology, 2004
This work was carried out to develop a rapid molecular profiling technique to screen ciliate populations in the rumen of sheep.DGGE was used to study the ciliate diversity in the rumen of sheep. There was considerable variation between sheep which were co-housed, and fed the same diet.
Regensbogenova, M., Pristas, P., Javorsky, P., Moon-van der Staay, S.Y., Staay, G.W.M. van der, Hackstein, J.H.P., Newbold, C.J., McEwan, N.R.   +7 more
openaire   +3 more sources

Alphaproteobacteria communities depend more on soil types than land managements

Acta Agriculturae Scandinavica. Section B, Soil and Plant Science, 2019
Soil properties and agricultural practices take a joint effect on the communities of soil bacteria. The aim of the present study was to survey Alphaproteobacterial communities as possible indicators of soil quality considering clay, loamy and sandy soils
Orsolya Gazdag, Tünde Takács, László Ködöböcz, Gergely Krett, Tibor Szili-Kovács   +4 more
doaj   +1 more source

Cyanobacterial community diversity in the sediments of the Pearl River Estuary in China

Scientia Marina, 2017
Cyanobacterial community diversity in the sediment of the Pearl River Estuary in China was evaluated in this study by denaturing gradient gel electrophoresis (DGGE) during the wet and dry seasons.
Fu-Lin Sun, You-Shao Wang, Mei-Lin Wu, Cui-Ci Sun   +3 more
doaj   +1 more source