Results 21 to 30 of about 20,908 (220)

Improved PCR-DGGE analysis by emulsion-PCR for the determination of food geographical origin: A case study on Greek PDO “avgotaracho Mesolonghiou”

open access: yesCurrent Research in Food Science, 2021
Greek avgotaracho Mesolonghiou (fish eggs from Flathead Mullet) is a highly valuable food product which holds Protected Destination of Origin status. The aim of this work was to use PCR-DGGE technique to examine whether there is a correlation between ...
Maria-Eleni Dimitrakopoulou   +2 more
doaj   +1 more source

Effect of legume-cereal mixtures on the diversity of bacterial communities in the rhizosphere

open access: yesPlant, Soil and Environment, 2012
Aboveground plant diversity is known to influence belowground diversity and ecosystem processes. However, there is little knowledge of soil microbial succession in legume-grass mixtures.
Y.J. Qiao   +5 more
doaj   +1 more source

Approach to analyze the diversity of myxobacteria in soil by semi-nested PCR-denaturing gradient gel electrophoresis (DGGE) based on taxon-specific gene. [PDF]

open access: yesPLoS ONE, 2014
The genotypic diversity of insoluble macromolecules degraded myxobacteria, provided an opportunity to discover new bacterial resources and find new ecological functions.
Baiyuan Li, Qing Yao, Honghui Zhu
doaj   +1 more source

Application of Denaturing Gradient Gel ElectrophoresisApplication of Denaturing Gradient Gel Electrophoresis(DGGE) Methods on Parent-Offspring Relationship of the Coral Pocillopora damicornis [PDF]

open access: yes, 2008
DGGE (Denaturing Gradient Gel Electrophoresis) is the most powerful methods for mutation detection currentlyavailable. In DGGE, DNA fragments of the same length but with different sequences can be separated.
Permata W, Diah, Hirose, M, Hidaka , M
core   +1 more source

Cyanobacterial community diversity in the sediments of the Pearl River Estuary in China

open access: yesScientia Marina, 2017
Cyanobacterial community diversity in the sediment of the Pearl River Estuary in China was evaluated in this study by denaturing gradient gel electrophoresis (DGGE) during the wet and dry seasons.
Fu-Lin Sun   +3 more
doaj   +1 more source

Evaluation of variations in plastid DNA non-coding regions in selected species of the genus Solanum

open access: yesCzech Journal of Genetics and Plant Breeding, 2017
The diversity of three non-coding plastid DNA loci (trnL/trnF spacer, trnV/16SrRNA spacer, trnL/trnL intron) was assessed in 16 Solanum L. species (135 individuals).
Vladimíra SEDLÁKOVÁ   +5 more
doaj   +1 more source

Emulsion PCR (ePCR) as a Tool to Improve the Power of DGGE Analysis for Microbial Population Studies

open access: yesMicroorganisms, 2020
To the authors’ knowledge, this is the first report of the use of emulsion-Polymerase chain reaction (e-PCR) coupled with denaturing gradient gel electrophoresis (DGGE) analysis. In the present work the effectiveness of ePCR in improving the power of the
Lucilla Iacumin   +3 more
doaj   +1 more source

Assessment of Bacterial Communities Within the Biofilm of Bladder Calculi in the Neurogenic Bladder Rat Model Following Spinal Cord Injury [PDF]

open access: yesInternational Neurourology Journal, 2022
Purpose To develop a rat model of bladder calculi in the neurogenic bladder following spinal cord injury (SCI) and assess bacterial communities within the biofilm of bladder calculi using denaturing gradient gel electrophoresis (DGGE).
Jeong Woo Lee   +3 more
doaj   +1 more source

Testing of primers for the study of cyanobacterial molecular diversity by DGGE [PDF]

open access: yesJournal of Microbiological Methods, 2006
Denaturing Gradient Gel electrophoresis (DGGE) is a PCR-based technique which is widely used in the study of microbial communities. Here, the use of the three specific 16S rRNA cyanobacterial specific primers CYA359F, CYA781R(a) and CYA781R(b) on the assessment of the molecular diversity of cyanobacterial communities is examined.
Boutte, C.   +3 more
openaire   +2 more sources

Autogenic versus environmental control during development of river biofilm [PDF]

open access: yes, 2009
In the natural environment, microbial community structure of river biofilm is controlled by biotic and abiotic factors. This study explored the capacity to manipulate the structure of microbial communities by modifying environmental conditions during the
Émilie Lyautey   +7 more
core   +1 more source

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