Approach to determine the diversity of Legionella species by nested PCR-DGGE in aquatic environments. [PDF]
PLoS ONE, 2017 In this study, we describe a nested PCR-DGGE strategy to detect Legionella communities from river water samples. The nearly full-length 16S rRNA gene was amplified using bacterial primer in the first step.
Wen-Chien Huang +7 more
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Evaluation of variations in plastid DNA non-coding regions in selected species of the genus Solanum
Czech Journal of Genetics and Plant Breeding, 2017 The diversity of three non-coding plastid DNA loci (trnL/trnF spacer, trnV/16SrRNA spacer, trnL/trnL intron) was assessed in 16 Solanum L. species (135 individuals).
Vladimíra SEDLÁKOVÁ +5 more
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Current Research in Food Science, 2021 Greek avgotaracho Mesolonghiou (fish eggs from Flathead Mullet) is a highly valuable food product which holds Protected Destination of Origin status. The aim of this work was to use PCR-DGGE technique to examine whether there is a correlation between ...
Maria-Eleni Dimitrakopoulou +2 more
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Approaches for diversity analysis of cultivable and non-cultivable bacteriain real soil
Plant, Soil and Environment, 2009 Until recently, investigators had no idea how accurately cultivated microorganisms represented the overall microbial diversity. The cultivation-dependent approach is limited by the fact that the overwhelming majority of microorganisms present in soil ...
P. Štursa +8 more
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Approach to analyze the diversity of myxobacteria in soil by semi-nested PCR-denaturing gradient gel electrophoresis (DGGE) based on taxon-specific gene. [PDF]
PLoS ONE, 2014 The genotypic diversity of insoluble macromolecules degraded myxobacteria, provided an opportunity to discover new bacterial resources and find new ecological functions.
Baiyuan Li, Qing Yao, Honghui Zhu
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Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste
International Journal of Renewable Energy Development, 2018 The natural microbial consortium from many sources widely used for hydrogen production. Type of substrate and operating conditions applied on the biodigesters of the natural consortium used as inoculum impact the variation of species and number of ...
Yumechris Amekan +4 more
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Fingerprinting sequence variation in ribosomal DNA of parasites by DGGE [PDF]
Molecular and Cellular Probes, 1996 Although there is a tendency for rDNA genes within a species to maintain sequence homogeneity, there can be significant levels of variation among rDNA repeat sequences within populations or individuals of a species as a consequence of mutation mechanisms.
Gasser, R., Nansen, P., Guldberg, P.
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Emulsion PCR (ePCR) as a Tool to Improve the Power of DGGE Analysis for Microbial Population Studies
Microorganisms, 2020 To the authors’ knowledge, this is the first report of the use of emulsion-Polymerase chain reaction (e-PCR) coupled with denaturing gradient gel electrophoresis (DGGE) analysis. In the present work the effectiveness of ePCR in improving the power of the
Lucilla Iacumin +3 more
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Assessment of Bacterial Communities Within the Biofilm of Bladder Calculi in the Neurogenic Bladder Rat Model Following Spinal Cord Injury [PDF]
International Neurourology Journal, 2022 Purpose To develop a rat model of bladder calculi in the neurogenic bladder following spinal cord injury (SCI) and assess bacterial communities within the biofilm of bladder calculi using denaturing gradient gel electrophoresis (DGGE).
Jeong Woo Lee +3 more
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Testing of primers for the study of cyanobacterial molecular diversity by DGGE [PDF]
Journal of Microbiological Methods, 2006 Denaturing Gradient Gel electrophoresis (DGGE) is a PCR-based technique which is widely used in the study of microbial communities. Here, the use of the three specific 16S rRNA cyanobacterial specific primers CYA359F, CYA781R(a) and CYA781R(b) on the assessment of the molecular diversity of cyanobacterial communities is examined.
Boutte, C. +3 more
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