Evaluation of variations in plastid DNA non-coding regions in selected species of the genus Solanum
The diversity of three non-coding plastid DNA loci (trnL/trnF spacer, trnV/16SrRNA spacer, trnL/trnL intron) was assessed in 16 Solanum L. species (135 individuals).
Vladimíra SEDLÁKOVÁ +5 more
doaj +1 more source
Emulsion PCR (ePCR) as a Tool to Improve the Power of DGGE Analysis for Microbial Population Studies
To the authors’ knowledge, this is the first report of the use of emulsion-Polymerase chain reaction (e-PCR) coupled with denaturing gradient gel electrophoresis (DGGE) analysis. In the present work the effectiveness of ePCR in improving the power of the
Lucilla Iacumin +3 more
doaj +1 more source
Assessment of Bacterial Communities Within the Biofilm of Bladder Calculi in the Neurogenic Bladder Rat Model Following Spinal Cord Injury [PDF]
Purpose To develop a rat model of bladder calculi in the neurogenic bladder following spinal cord injury (SCI) and assess bacterial communities within the biofilm of bladder calculi using denaturing gradient gel electrophoresis (DGGE).
Jeong Woo Lee +3 more
doaj +1 more source
Repeated pulse feeding induces functional stability in anaerobic digestion [PDF]
Anaerobic digestion is an environmental key technology in the future bio-based economy. To achieve functional stability, a minimal microbial community diversity is required. This microbial community should also have a certain “elasticity”, i.e.
Aiyuk +29 more
core +1 more source
Optimization of the DGGE band identification method
Denaturant gradient gel electrophoresis (DGGE) enables insight into the diversity of the studied microbial communities on the basis of separation of PCR amplification products according to their nucleotide sequence composition. However, the success of the method is accompanied by the inherent appearance of various sequence artifacts that bias the ...
Kušar, Darja, Avguštin, Gorazd
openaire +3 more sources
How endo- is endo-?: surface sterilization of delicate samples: a Bryopsis (Bryopsidales, Chlorophyta) case study [PDF]
In the search for endosymbiotic bacteria, elimination of ectosymbionts is a key point of attention. Commonly, the surface of the host itself or the symbiotic structures are sterilized with aggressive substances such as chlorine or mercury derivatives ...
De Clerck, Olivier +3 more
core +2 more sources
Identification of yeasts present in artisanal yoghurt and traditionally fermented milks consumed in the northern part of Cameroon [PDF]
Bacteria generally ferment milk but, sometimes, yeasts are found in fermented milks. The presence of these yeasts in the microbial community of some fermented milks could be intentional or accidental.
Maïworé, Justine +3 more
core +1 more source
DGGE-based detection method for Quahog Parasite Unknown (QPX) [PDF]
Quahog Parasite Unknown (QPX) is a significant cause of hard clam Mercenaria mercenaria mortality along the northeast coast of the United States. It infects both wild and cultured clams, often annually in plots that are heavily farmed. Subclinically infected clams can be identified by histological examination of the mantle tissue, but there is ...
Gast, Rebecca J. +5 more
openaire +3 more sources
Molekulski pristopi pri raziskavah arbuskularne mikorize
Arbuskularna mikoriza se je razvila v koevoluciji s kopenskimi rastlinami. Čeprav predstavlja eno najbolj razširjenih simbioz na kopnem, še vedno obstajajo številna odprta vprašanja o biologiji arbuskularnih mikoriznih gliv ter njihovi ekološki vlogi v ...
Irena MAČEK
doaj +1 more source
The aim of the study was to determine the Actinobacteria structure in cultivated (C) versus non-cultivated (NC) soils divided into three groups (autogenic, hydrogenic, lithogenic) with consideration its formation process in order to assess the ...
Agnieszka Wolińska +6 more
doaj +1 more source

