Results 41 to 50 of about 28,160 (153)
Role of PKD2 in Rheotaxis in Dictyostelium [PDF]
The sensing of mechanical forces modulates several cellular responses as adhesion, migration and differentiation. Transient elevations of calcium concentration play a key role in the activation of cells following mechanical stress, but it is still unclear how eukaryotic cells convert a mechanical signal into an ion flux.
Lima, W. C.+3 more
openaire +6 more sources
Polymorphic members of the lag gene family mediate kin discrimination in Dictyostelium [PDF]
Self and kin discrimination are observed in most kingdoms of life and are mediated by highly polymorphic plasma membrane proteins. Sequence polymorphism, which is essential for effective recognition, is maintained by balancing selection.
Benabentos, Rocio+10 more
core +1 more source
Background Anandamide (Arachidonoyl ethanolamide) is a potent bioactive lipid studied extensively in humans, which regulates several neurobehavioral processes including pain, feeding and memory.
Neelamegan Dhamodharan+3 more
doaj +1 more source
Multi-color fluorescence live-cell imaging in Dictyostelium discoideum
The cellular slime mold Dictyostelium discoideum, a member of the Amoebozoa, has been extensively studied in cell and developmental biology. D. discoideum is unique in that they are genetically tractable, with a wealth of data accumulated over half a ...
Hidenori Hashimura+8 more
doaj +1 more source
Resistance of
Background Saponin is a mild detergent commonly used to permeabilize cells prior to immunofluorescence labeling of intracellular proteins. It has previously been used to that effect in Dictyostelium discoideum amoebae.
Cosson Pierre, Mercanti Valentina
doaj +1 more source
Functional Analysis of Spontaneous Cell Movement under Different Physiological Conditions [PDF]
Cells can show not only spontaneous movement but also tactic responses to environmental signals. Since the former can be regarded as the basis to realize the latter, playing essential roles in various cellular functions, it is important to investigate ...
A Czir'ok+15 more
core +3 more sources
The loading controls used for quantitative immunoblotting of mammalian proteins are not appropriate for use with Dictyostelium discoideum. Actin levels, for example, change greatly during Dictyostelium development.
Andrew J. Davidson+2 more
doaj +1 more source
Microsatellites are repetitive sequences commonly found in the genomes of higher organisms. These repetitive sequences are prone to expansion or contraction, and when microsatellite expansion occurs in the regulatory or coding regions of genes this can ...
Felicia N. Williams+3 more
doaj +1 more source
ABSTRACT This review provides an in‐depth exploration of capillary electrophoresis–mass spectrometry (CE–MS) in biomolecular research from 2020 to 2024. CE–MS emerges as a versatile and powerful tool due to its numerous advantages, facilitating the analysis of various biomolecules, including proteins, peptides, oligonucleotides, and other metabolites ...
Laura Pont+2 more
wiley +1 more source
A gradient method for the quantitative analysis of cell movement and tissue flow and its application to the analysis of multicellular Dictyostelium development [PDF]
We describe the application of a novel image processing method, which allows quantitative analysis of cell and tissue movement in a series of digitized video images. The result is a vector velocity field showing average direction and velocity of movement
Miike, Hidetoshi+3 more
core +1 more source