Results 41 to 50 of about 28,160 (153)

Role of PKD2 in Rheotaxis in Dictyostelium [PDF]

open access: yesPLoS ONE, 2014
The sensing of mechanical forces modulates several cellular responses as adhesion, migration and differentiation. Transient elevations of calcium concentration play a key role in the activation of cells following mechanical stress, but it is still unclear how eukaryotic cells convert a mechanical signal into an ion flux.
Lima, W. C.   +3 more
openaire   +6 more sources

Polymorphic members of the lag gene family mediate kin discrimination in Dictyostelium [PDF]

open access: yes, 2009
Self and kin discrimination are observed in most kingdoms of life and are mediated by highly polymorphic plasma membrane proteins. Sequence polymorphism, which is essential for effective recognition, is maintained by balancing selection.
Benabentos, Rocio   +10 more
core   +1 more source

Identification and recombinant expression of anandamide hydrolyzing enzyme from Dictyostelium discoideum

open access: yesBMC Microbiology, 2012
Background Anandamide (Arachidonoyl ethanolamide) is a potent bioactive lipid studied extensively in humans, which regulates several neurobehavioral processes including pain, feeding and memory.
Neelamegan Dhamodharan   +3 more
doaj   +1 more source

Multi-color fluorescence live-cell imaging in Dictyostelium discoideum

open access: yesCell Structure and Function
The cellular slime mold Dictyostelium discoideum, a member of the Amoebozoa, has been extensively studied in cell and developmental biology. D. discoideum is unique in that they are genetically tractable, with a wealth of data accumulated over half a ...
Hidenori Hashimura   +8 more
doaj   +1 more source

Resistance of Dictyostelium discoideum membranes to saponin permeabilization

open access: yesBMC Research Notes, 2010
Background Saponin is a mild detergent commonly used to permeabilize cells prior to immunofluorescence labeling of intracellular proteins. It has previously been used to that effect in Dictyostelium discoideum amoebae.
Cosson Pierre, Mercanti Valentina
doaj   +1 more source

Functional Analysis of Spontaneous Cell Movement under Different Physiological Conditions [PDF]

open access: yes, 2008
Cells can show not only spontaneous movement but also tactic responses to environmental signals. Since the former can be regarded as the basis to realize the latter, playing essential roles in various cellular functions, it is important to investigate ...
A Czir'ok   +15 more
core   +3 more sources

The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum

open access: yesBioTechniques, 2013
The loading controls used for quantitative immunoblotting of mammalian proteins are not appropriate for use with Dictyostelium discoideum. Actin levels, for example, change greatly during Dictyostelium development.
Andrew J. Davidson   +2 more
doaj   +1 more source

Insights on Microsatellite Characteristics, Evolution, and Function From the Social Amoeba Dictyostelium discoideum

open access: yesFrontiers in Neuroscience, 2022
Microsatellites are repetitive sequences commonly found in the genomes of higher organisms. These repetitive sequences are prone to expansion or contraction, and when microsatellite expansion occurs in the regulatory or coding regions of genes this can ...
Felicia N. Williams   +3 more
doaj   +1 more source

A Comprehensive Review on Capillary Electrophoresis–Mass Spectrometry in Advancing Biomolecular Research

open access: yesELECTROPHORESIS, EarlyView.
ABSTRACT This review provides an in‐depth exploration of capillary electrophoresis–mass spectrometry (CE–MS) in biomolecular research from 2020 to 2024. CE–MS emerges as a versatile and powerful tool due to its numerous advantages, facilitating the analysis of various biomolecules, including proteins, peptides, oligonucleotides, and other metabolites ...
Laura Pont   +2 more
wiley   +1 more source

A gradient method for the quantitative analysis of cell movement and tissue flow and its application to the analysis of multicellular Dictyostelium development [PDF]

open access: yes, 1994
We describe the application of a novel image processing method, which allows quantitative analysis of cell and tissue movement in a series of digitized video images. The result is a vector velocity field showing average direction and velocity of movement
Miike, Hidetoshi   +3 more
core   +1 more source

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