Results 41 to 50 of about 52,165 (246)

Web-enabled knowledge-based analysis of genetic data [PDF]

, 2001
We present a web-based implementation of GenePath, an intelligent assistant tool for data analysis in functional genomics. GenePath considers mutant data and uses expert-defined patterns to find gene-to-gene or gene-to-outcome relations.
Bratko, Ivan, Demšar, Janez, Halter, John A., Juvan, Peter, Kuspa, Adam, Shaulsky, Gad, Zupan, Blaz   +6 more
core   +2 more sources

Multiscale modeling of oscillations and spiral waves in Dictyostelium populations

, 2014
Unicellular organisms exhibit elaborate collective behaviors in response to environmental cues. These behaviors are controlled by complex biochemical networks within individual cells and coordinated through cell-to-cell communication.
Gregor, Thomas, Mehta, Pankaj, Noorbakhsh, Javad, Schwab, David, Sgro, Allyson   +4 more
core   +1 more source

Patterns of cell movement within the Dictyostelium slug revealed by cell type-specific, surface labeling of living cells [PDF]

, 1994
There are cells acattered in the rear, prespore region of the Dictyostelium slug that share many of the properties of the prestalk cells and that are therefore called anterior-like cells (ALCs).
Akiyama, Blaschke, Bonner, Bonner, Buhl, Ceccarelli, Chung, Devine, Early, Early, Early, Evan, Francis, Gaskell, Gregg, Gregg, Hadwiger, Harlow, Harwood, Howard, Jermyn, Jermyn, Kakutani, McRobbie, Montell, Niehrs, Nomura, Raper, Raper, Rubin, Salser, Siegert, Siegert, Siegert, Steinbock, Sternfeld, Sternfeld, Sternfeld, Takeuchi, Ti, Watts, Williams   +41 more
core   +1 more source

Role of PKD2 in Rheotaxis in Dictyostelium [PDF]

PLoS ONE, 2014
The sensing of mechanical forces modulates several cellular responses as adhesion, migration and differentiation. Transient elevations of calcium concentration play a key role in the activation of cells following mechanical stress, but it is still unclear how eukaryotic cells convert a mechanical signal into an ion flux.
Lima, Wanessa Cristina, Vinet, Adrien, Pieters, Jean, Cosson, Pierre   +3 more
openaire   +6 more sources

Protection of spores from ultraviolet-C irradiation by auto-fluorescent substances in the spore mass of the cellular slime mold Dictyostelium discoideum

Pteridines, 2014
In this study, native spores surrounded by fluorescent substances in the spore mass of Dictyostelium discoideum were found to be resistant to relatively strong ultraviolet-C (UV-C) irradiation (2880 J/m2).
Uchiyama Saburo, Hatakeyama Ikuo
doaj   +1 more source

Digital image processing of optical density wave propagation in Dictyostelium discoideum and analysis of the effects of caffeine and ammonia [PDF]

, 1989
Waves of chemotactic movement during the early phase of aggregation in Dictyostelium discoideum were analyzed by digital image processing in a manner that immediately shows the following parameters: wave propagation velocity, period length, wave ...
Siegert, Florian, Weijer, Cornelis J.
core   +1 more source

Quantitative analysis by flow cytometry of green fluorescent protein-tagged human phenylalanine hydroxylase expressed in Dictyostelium

Pteridines, 2017
We have developed a fluorescence assay system to monitor the protein levels of human phenylalanine hydroxylase (hPAH). Wild-type (WT) and three mutant hPAHs (I65T, L255V, and S349L) were expressed as green fluorescent protein (GFP)-tagged forms in a PAH ...
Kim Hye-Lim, Ryu Hyun-Chul, Park Young Shik   +2 more
doaj   +1 more source

Resistance of Dictyostelium discoideum membranes to saponin permeabilization

BMC Research Notes, 2010
Background Saponin is a mild detergent commonly used to permeabilize cells prior to immunofluorescence labeling of intracellular proteins. It has previously been used to that effect in Dictyostelium discoideum amoebae.
Cosson Pierre, Mercanti Valentina
doaj   +1 more source

SILAC-based proteomic quantification of chemoattractant-induced cytoskeleton dynamics on a second to minute timescale [PDF]

, 2014
Cytoskeletal dynamics during cell behaviours ranging from endocytosis and exocytosis to cell division and movement is controlled by a complex network of signalling pathways, the full details of which are as yet unresolved.
A Bagorda, A Kortholt, A Kortholt, A Para, A Shevchenko, AJ Saldanha, AT Sasaki, C Orelio, CL Chen, CL Manahan, DM Veltman, DM Veltman, DM Veltman, EC Rericha, EL de Hostos, F Friedberg, F Vazquez, G Vlahou, H Cai, H Kae, HR Bourne, I Marin, J Condeelis, J Cox, J Faix, J Riedl, J Schindelin, J Yan, JF Cote, JF Cote, JW Han, JY Kim, KF Swaney, L Bosgraaf, L Bosgraaf, L Bosgraaf, L Chen, M Affolter, M Brenner, M de la Roche, M Patel, MJ de Hoon, MK Vartiainen, MR Lee, MS Boguski, N Ibarra, N Meller, OD Weiner, R Insall, RH Insall, RJ Eddy, S Hanna, S Levi, SE Ong, SJ Allen, SL Blagg, TJ Jeon, WN van Egmond, X Xu, Y Yang, YC Wu   +60 more
core   +3 more sources

The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum

BioTechniques, 2013
The loading controls used for quantitative immunoblotting of mammalian proteins are not appropriate for use with Dictyostelium discoideum. Actin levels, for example, change greatly during Dictyostelium development.
Andrew J. Davidson, Jason S. King, Robert H. Insall   +2 more
doaj   +1 more source