Results 31 to 40 of about 721,346 (281)
Direct-TRI: High-throughput RNA-extracting Method for all Stages of Zebrafish Development
Bio-Protocol, 2021 Recent popularization of next-generation sequencing enables conducting easy transcriptome analysis. Nevertheless, substantial RNA isolation work prior to RNA sequencing, as well as the high cost involved, still makes the routine use of large-scale ...
Kota Ujibe +3 more
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Advancing transcriptome platforms [PDF]
, 2011 During the last decade of years, remarkable technological innovations have emerged that allow the direct or indirect determination of the transcriptome at unprecedented scale and speed.
Shuobo Shi
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The Microglial Sensome Revealed by Direct RNA Sequencing [PDF]
, 2014 Microglia, the principal neuroimmune sentinels of the brain, continuously sense changes in their environment and respond to invading pathogens, toxins and cellular debris.
Borowsky, Mark +6 more
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An informatics approach to distinguish RNA modifications in nanopore direct RNA sequencing
Genomics, 2022 Modifications in RNA can influence their structure, function, and stability and play essential roles in gene expression and regulation. Methods to detect RNA modifications rely on biophysical techniques such as chromatography or mass spectrometry, which are low throughput, or on high throughput short-read sequencing techniques based on selectively ...
Ramasamy, Soundhar +8 more
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On the design and prospects of direct RNA sequencing [PDF]
Briefings in Functional Genomics, 2017 Throughout the past nearly a decade, the application of high-throughput sequencing to RNA molecules in the form of RNA sequencing (RNA-seq) and its many variations has revolutionized transcriptomic studies by enabling researchers to take a simultaneously deep and truly global look into the transcriptome.
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Nanopore direct RNA sequencing of human transcriptomes reveals the complexity of mRNA modifications and crosstalk between regulatory features [PDF]
Cell GenomicsLy P Vu
exaly +2 more sources
RnaseIII and T4 Polynucleotide Kinase Sequence Biases and Solutions During RNA-Seq Library Construction [PDF]
, 2013 Background: RNA-seq is a next generation sequencing method with a wide range of applications including single nucleotide polymorphism (SNP) detection, splice junction identification, and gene expression level measurement.
Changhoon Lee +5 more
core +3 more sources
Direct RNA Sequencing Unfolds the Complex Transcriptome of Vibrio parahaemolyticus
mSystems, 2021 Conventional bacterial genome annotation provides information about coding sequences but ignores untranslated regions and operons. However, untranslated regions contain important regulatory elements as well as targets for many regulatory factors, such as
Mohamad Al kadi +7 more
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Energy directed folding of RNA sequences
Nucleic Acids Research, 1984 A modification of Nussinov's algorithm (1) for (planar) secondary structure generation is described. Our algorithm postpones decisions on matches involving destabiling loops until they prove to be energetically more favourable than more local matches. We present, moreover, an alternative way of representing secondary structures which avoids unwarranted
Paulien Hogeweg, Ben Hesper
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Genome Biology, 2023 Single-molecule detection and phasing of A-to-I RNA editing events remain an unresolved problem. Long-read and PCR-free nanopore native RNA sequencing offers a great opportunity for direct RNA editing detection.
Longxian Chen +9 more
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