Results 31 to 40 of about 4,264,667 (244)
Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species [PDF]
, 2012 Conclusion: By carefully choosing the appropriate RNA extraction method, whole blood can become a valuable source for high-throughput applications like expression arrays or transcriptome sequencing from natural populations.
Thalmann, Olaf +3 more
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Frontiers in Microbiology, 2022 High-throughput sequencing (HTS) has become an important tool for plant virus detection and discovery. Nanopore sequencing has been rapidly developing in the recent years and offers new possibilities for fast diagnostic applications of HTS.
Anja Pecman +7 more
doaj +1 more source
Mapping of RNA Modifications by Direct Nanopore Sequencing and JACUSA2
, 2023 RNA modifications exist in all kingdom of life. Several different types of base or ribose modifications are now summarized under the term the ”epitranscriptome”. With the advent of high-throughput sequencing technologies, much progress has been made in understanding RNA modification biology and how these modifications can influence many aspects of RNA ...
Amina, Lemsara +2 more
openaire +3 more sources
Direct RNA-sequencing analysis.
, 2022 (A) Cluster analysis of differentially expressed transcripts in the three different genotypes. (B) Distribution of m6A methylations detected by direct RNA-sequencing. (C) Logo of the conserved m6A sequence motif detected by direct RNA-sequencing.
Ulla Dolde (2576458) +12 more
core +1 more source
Genomics, Proteomics & Bioinformatics, 2023 Transcriptome analysis based on high-throughput sequencing of a cDNA library has been widely applied to functional genomic studies. However, the cDNA dependence of most RNA sequencing techniques constrains their ability to detect base modifications on ...
Feng Yu +7 more
doaj +1 more source
FASTdRNA: a workflow for the analysis of ONT direct RNA sequencing
Bioinformatics Advances, 2023 Abstract Motivation Direct RNA-seq (dRNA-seq) using Oxford Nanopore Technology (ONT) has revolutionized transcript mapping by offering enhanced precision due to its long-read length. Unlike traditional techniques, dRNA-seq eliminates the need for PCR amplification, reducing the impact of GC bias, and ...
Xiaofeng Chen +5 more
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Development of methods for the analysis of deep sequencing data; applications to the discovery of functions of RNA-binding proteins [PDF]
, 2014 With the recent advances in nucleotide sequencing technologies, it became easy to generate tens of millions of reads with genome- or transcriptome-wide distribution with reduced cost and high accuracy.
Bilen, Biter
core +1 more source
Nanopore sequencing of RNA and cDNA molecules in Escherichia coli [PDF]
, 2021 High-throughput sequencing dramatically changed our view of transcriptome architectures and allowed for ground-breaking discoveries in RNA biology. Recently, sequencing of full-length transcripts based on the single-molecule sequencing platform from ...
Grohmann, Dina +2 more
core +1 more source
A novel method for the capture-based purification of whole viral native RNA genomes
AMB Express, 2019 Current technologies for targeted characterization and manipulation of viral RNA primarily involve amplification or ultracentrifugation with isopycnic gradients of viral particles to decrease host RNA background. The former strategy is non-compatible for
Cedric Chih Shen Tan +4 more
doaj +1 more source
Detection and quantification of 5moU RNA modification from direct RNA sequencing data
Current Genomics, 2023 Abstract Background Chemically modified therapeutic mRNAs have gained its momentum recently. In addition to commonly used modifications (e.g., pseudouridine), 5moU is considered a promising substitution of uridine in therapeutic mRNAs. Accurate identification of 5-Methoxyuridine (5moU) would be crucial for the study and quality control of ...
Li, Jiayi +6 more
openaire +2 more sources