Results 41 to 50 of about 4,264,667 (244)

DeepEdit: single-molecule detection and phasing of A-to-I RNA editing events using nanopore direct RNA sequencing

open access: yesGenome Biology, 2023
Single-molecule detection and phasing of A-to-I RNA editing events remain an unresolved problem. Long-read and PCR-free nanopore native RNA sequencing offers a great opportunity for direct RNA editing detection.
Longxian Chen   +9 more
doaj   +1 more source

Computational analysis of next generation sequencing data : from transcription start sites in bacteria to human non-coding RNAS [PDF]

open access: yes, 2014
The advent of next generation sequencing (NGS) technologies has revolutionized the field of molecular biology by providing a wealth of sequence data. “Transcriptomics”, which aims to identify and annotate the complete set of RNA molecules transcribed ...
Jorjani, Hadi
core   +1 more source

RNA-specific ribonucleotidyl transferases [PDF]

open access: yes, 2007
RNA-specific nucleotidyl transferases (rNTrs) are a diverse family of template-independent polymerases that add ribonucleotides to the 3'-ends of RNA molecules.
Keller, W.   +3 more
core   +1 more source

Applications and potentials of nanopore sequencing in the (epi)genome and (epi)transcriptome era

open access: yesThe Innovation, 2021
Summary: The Human Genome Project opened an era of (epi)genomic research, and also provided a platform for the development of new sequencing technologies.
Shangqian Xie   +7 more
doaj   +1 more source

Systematic comparison of tools used for m6A mapping from nanopore direct RNA sequencing

open access: yesNature Communications, 2023
Direct RNA sequencing using nanopore platform can be used to detect N6-methyladenosine (m6A) modifications on mRNAs. Here the authors systematically compare tools used for m6A detection from nanopore direct sequencing.
Zhen-Dong Zhong   +11 more
doaj   +1 more source

Soil Metatranscriptomics: An Improved RNA Extraction Method Toward Functional Analysis Using Nanopore Direct RNA Sequencing

open access: yesPhytobiomes Journal, 2023
Soil microbes play an undeniable role in sustainable agriculture, plant health, and soil management. A deeper understanding of soil microbial composition and function has been gained through next-generation sequencing.
Abdonaser Poursalavati   +3 more
doaj   +1 more source

A complete RNA-sequencing pipeline

open access: yes, 2015
<p>First public release of the pipeline used for analysis of RNA-sequencing (RNA-seq) data extended from (Nalpas N.C. et al, http://dx.doi.org/10.5281/zenodo.12474) to include an additional experimental group and novel analyses.</p> <p>
Rue-Albrecht, Kevin, Nalpas, Nicolas C.
core   +1 more source

A comprehensive evaluation of alignment algorithms in the context of RNA-seq. [PDF]

open access: yes, 2012
Transcriptome sequencing (RNA-Seq) overcomes limitations of previously used RNA quantification methods and provides one experimental framework for both high-throughput characterization and quantification of transcripts at the nucleotide level.
Friedel, Caroline C.   +8 more
core   +2 more sources

Ovarian Sex Cord Stromal Tumors in Children and Adolescents—The European Standard Clinical Practice Recommendations

open access: yesPediatric Blood &Cancer, EarlyView.
ABSTRACT As part of the European Cooperative Study Group for Paediatric Rare Tumours initiative, we developed standard clinical practice guidelines for ovarian sex cord stromal tumors, based on comprehensive national and international cohort analyses, literature review, and a final expert consensus conference.
Dominik T. Schneider   +15 more
wiley   +1 more source

Direct chemical method for sequencing RNA. [PDF]

open access: yesProceedings of the National Academy of Sciences, 1979
Four different base-specific chemical reactions generate a means of directly sequencing RNA terminally labeled with 32P. After a partial, specific modification of each kind of RNA base, an amine-catalyzed strand scission generates labeled fragments whose lengths determine the position of each nucleotide in the sequence.
openaire   +2 more sources

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