Results 61 to 70 of about 721,346 (281)

Soil Metatranscriptomics: An Improved RNA Extraction Method Toward Functional Analysis Using Nanopore Direct RNA Sequencing

Phytobiomes Journal, 2023
Soil microbes play an undeniable role in sustainable agriculture, plant health, and soil management. A deeper understanding of soil microbial composition and function has been gained through next-generation sequencing.
Abdonaser Poursalavati, Vahid J. Javaran, Isabelle Laforest-Lapointe, Mamadou L. Fall   +3 more
doaj   +1 more source

Poly(a) selection introduces bias and undue noise in direct RNA-sequencing

BMC Genomics, 2022
Background Genome-wide RNA-sequencing technologies are increasingly critical to a wide variety of diagnostic and research applications. RNA-seq users often first enrich for mRNA, with the most popular enrichment method being poly(A) selection.
Marcus J. Viscardi, Joshua A. Arribere
doaj   +1 more source

Artificial escape from XCI by DNA methylation editing of the CDKL5 gene. [PDF]

, 2020
A significant number of X-linked genes escape from X chromosome inactivation and are associated with a distinct epigenetic signature. One epigenetic modification that strongly correlates with X-escape is reduced DNA methylation in promoter regions. Here,
Anderson, Johnathon D, Buchanan, Fiona KB, Cameron, David, Carter, Jasmine L, Coggins, Nicole B, Deng, Peter, Fink, Kyle D, Gonzalez, Casiana E, Halmai, Julian ANM, Halmai, Julian ANM, Lock, Samantha R, Nolta, Jan, O'Geen, Henriette, Segal, David J, Waldo, Jennifer J   +14 more
core   +1 more source

MicroRNA-like RNAs from the same miRNA precursors play a role in cassava chilling responses [PDF]

, 2017
MicroRNAs (miRNAs) are known to play important roles in various cellular processes and stress responses. MiRNAs can be identified by analyzing reads from high-throughput deep sequencing.
Chen, Xin, Peng, Ming, Xia, Jing, Zeng, Changying, Zhang, Weixiong, Zhou, Yufei   +5 more
core   +3 more sources

Personalized Selumetinib Dosing in Pediatric Neurofibromatosis Type 1: Insights From a Pilot Therapeutic Drug Monitoring Study

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Objective To evaluate selumetinib exposure using therapeutic drug monitoring (TDM) in pediatric patients with neurofibromatosis type 1 (NF1) and plexiform neurofibromas (PN), assess interpatient pharmacokinetic variability, and explore the relationship between drug exposure, clinical response, and adverse effects.
Janka Kovács, Zoltán Köllő, Balázs Gellért Karvaly, Mahmoud Obeidat, Rita Nagy, Péter Hegyi, Brigitta Teutsch, Eszter Tuboly, Miklós Garami   +8 more
wiley   +1 more source

Evaluation of experimental design and computational parameter choices affecting analyses of ChIP-seq and RNA-seq data in undomesticated poplar trees. [PDF]

, 2014
BackgroundOne of the great advantages of next generation sequencing is the ability to generate large genomic datasets for virtually all species, including non-model organisms.
Filkov, Vladimir, Groover, Andrew, Liu, Lijun, Missirian, Victor, Zinkgraf, Matthew   +4 more
core   +2 more sources

Two Faces of NOTCH1 in Childhood Lymphoblastic T‐Cell Neoplasia: Prognostic Divergence of Mutational and Structural Aberrations

Pediatric Blood &Cancer, EarlyView.
ABSTRACT In pediatric patients, T‐cell lymphoblastic lymphoma (T‐LBL) survival exceeds 80%. Relapse remains associated with limited curative options. Frontline treatment is largely extrapolated from T‐cell acute lymphoblastic leukemia (T‐ALL) treatment, reflecting the ongoing debate, whether both entities represent distinct diseases or variants within ...
Marie C. Heider, Amelie Alfert, Marc Hotfilder, Birgit Burkardt, Marcel te Vrugt   +4 more
wiley   +1 more source

Direct RNA sequencing reveals m6A modifications on adenovirus RNA are necessary for efficient splicing

Nature Communications, 2020
Adenovirus transcripts contain N6-methyladenosine (m6A) modification. Here the authors profile m6A modification sites on adenovirus mRNAs using Illumina meRIP-Seq and nanopore direct RNA sequencing, and showcase a role for m6A in splicing of viral late ...
Alexander M. Price, Katharina E. Hayer, Alexa B. R. McIntyre, Nandan S. Gokhale, Jonathan S. Abebe, Ashley N. Della Fera, Christopher E. Mason, Stacy M. Horner, Angus C. Wilson, Daniel P. Depledge, Matthew D. Weitzman   +10 more
doaj   +1 more source

Rosetta Brains: A Strategy for Molecularly-Annotated Connectomics [PDF]

, 2014
We propose a neural connectomics strategy called Fluorescent In-Situ Sequencing of Barcoded Individual Neuronal Connections (FISSEQ-BOINC), leveraging fluorescent in situ nucleic acid sequencing in fixed tissue (FISSEQ).
Boyden, Edward S, Church, George M, Daugharthy, Evan R, Kalhor, Reza, Kebschull, Justus M, Kording, Konrad P, Lee, Je Hyuk, Marblestone, Adam H, Mishchenko, Yuriy, Peikon, Ian D, Shipman, Seth L, Zador, Anthony M   +11 more
core  

Hepatitis C virus cell-cell transmission and resistance to direct-acting antiviral agents [PDF]

, 2014
Hepatitis C virus (HCV) is transmitted between hepatocytes via classical cell entry but also uses direct cell-cell transfer to infect neighboring hepatocytes.
A Owsianka, A Sigal, Aleem Siddiqui, Arvind H. Patel, AS Lok, AW Tarr, B Bartolini, B Sainz Jr, C Hezode, C Lee, C Nathan, C Sarrazin, CL Brimacombe, CM Lange, CT Jones, D Masson, D Trono, EJ Gane, Eric Soulier, F Kuonen, F Poordad, F Poordad, Fei Xiao, François Habersetzer, G Koutsoudakis, H Kwon, H Yan, H Zhu, Heidi Barth, I Fofana, I Fofana, I Fofana, IA Abela, IM Jacobson, Isabel Fofana, J Grove, J Lupberger, J Witteveldt, J Zhong, Jens Bukh, JG McHutchison, JM Gottwein, JM Gottwein, JM Pawlotsky, JM Pawlotsky, JM Pawlotsky, JM Timpe, L Rong, L Zona, Laura Heydmann, LW Meredith, MB Zeisel, MB Zeisel, Michel Doffoël, Mirjam B. Zeisel, MN Zahid, MS Sulkowski, MT Catanese, N Shi, O Lenz, P Zhong, PM Trembling, Q Sattentau, S Haid, T Pietschmann, T Pietschmann, T Wakita, Thomas F. Baumert, TJ Liang, TK Scheel, V Soriano, VD Cataldo, ZY Keck   +72 more
core   +7 more sources