Results 71 to 80 of about 4,264,667 (244)

RNA-sequencing about yin.xlsx

, 2022
Through RNA sequencing, we explained the mechanism of maintaining the intestinal barrier by activating NRF2 in obese mice and thus alleviating metabolic ...
Ruopeng Yin (12998628)
core   +1 more source

Assessment of nanopore RNA modification calling in human cell lines and synthetic systems

Genome Biology
Background Nanopore technology enables the direct sequencing of intact RNA molecules allowing for the detection of native chemical modifications. In 2024, Oxford Nanopore Technologies updated direct RNA sequencing from RNA002 to RNA004 platform as well ...
Neda Ghohabi Esfahani, Andrew J. Stein, Stuart Akeson, Talia Tzadikario, Connor Powell, Pooria Daneshvar Kakhaki, Miten Jain   +6 more
doaj   +1 more source

Full-length direct RNA sequencing uncovers stress granule-dependent RNA decay upon cellular stress

eLife
Cells react to stress by triggering response pathways, leading to extensive alterations in the transcriptome to restore cellular homeostasis. The role of RNA metabolism in shaping the cellular response to stress is vital, yet the global changes in RNA ...
Showkat Ahmad Dar, Sulochan Malla, Vlastimil Martinek, Matthew John Payea, Christopher Tai-Yi Lee, Jessica Martin, Aditya Jignesh Khandeshi, Jennifer L Martindale, Cedric Belair, Manolis Maragkakis   +9 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Enhanced detection of RNA modifications in Escherichia coli utilizing direct RNA sequencing

Cell Reports: Methods
Summary: RNA modifications play crucial roles in prokaryotic cellular processes. In this study, we found that the recent advances in direct RNA sequencing have improved yield, accuracy, and signal-to-noise ratio in bacterial samples.
Zhihao Guo, Yanwen Shao, Lu Tan, Beifang Lu, Xin Deng, Sheng Chen, Runsheng Li   +6 more
doaj   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Probing the epitranscriptome and RNA damage with nanopore direct RNA sequencing

RNA
Nanopore direct RNA sequencing (DRS) is revolutionizing our ability to analyze the epitranscriptome to evaluate nucleoside modifications in both cellular and synthetic RNA. The process involves minimal handling of fragile RNA strands, one round of reverse transcription to provide a DNA:RNA duplex, and library preparation to directly read nucleotides ...
Aaron M. Fleming, Cynthia J. Burrows
openaire   +2 more sources

RNA Sequencing in Schizophrenia

, 2015
Schizophrenia (SCZ) is a serious psychiatric disorder that affects 1% of general population and places a heavy burden worldwide. The underlying genetic mechanism of SCZ remains unknown, but studies indicate that the disease is associated with a global ...
Shaolei Teng, Xin Li
core   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Massively parallel characterization of engineered transcript isoforms using direct RNA sequencing

Nature Communications, 2022
Transcriptional terminators are generally viewed as hard endpoints for transcribing RNA polymerases. Here, the authors reimagine terminators as transcriptional valves with predictable read through.
Matthew J. Tarnowski, Thomas E. Gorochowski   +1 more
doaj   +1 more source