Results 81 to 90 of about 4,264,667 (244)

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

General lack of global dosage compensation in ZZ/ZW systems? Broadening the perspective with RNA-seq [PDF]

, 2011
Background Species with heteromorphic sex chromosomes face the challenge of large-scale imbalance in gene dose. Microarray-based studies in several independent male heterogametic XX/XY systems suggest that dosage compensation mechanisms are in place to ...
Bryk, Jaroslaw,, Bryk, Jarek, Wolf, Jochen B. W.,, Wolf, Jochen BW, Bryk Jarosław, Wolf, Jochen B. W., Jochen BW Wolf, Bryk, J., Bryk, Jarosłav, Wolf, J., Jarosław Bryk, Wolf Jochen BW   +11 more
core   +2 more sources

Raw signal segmentation for estimating RNA modification from Nanopore direct RNA sequencing data

eLife
Estimating RNA modifications from Nanopore direct RNA sequencing data is a critical task for the RNA research community. However, current computational methods often fail to deliver satisfactory results due to inaccurate segmentation of the raw signal ...
Guangzhao Cheng, Aki Vehtari, Lu Cheng
doaj   +1 more source

Direct RNA targeted in situ sequencing for transcriptomic profiling in tissue

Scientific Reports, 2022
Highly multiplexed spatial mapping of transcripts within tissues allows for investigation of the transcriptomic and cellular diversity of mammalian organs previously unseen.
Hower Lee, Sergio Marco Salas, Daniel Gyllborg, Mats Nilsson   +3 more
doaj   +1 more source

Epigenetic reprogramming of lineage switching in cancer

FEBS Letters, EarlyView.
Cancer cells rarely commit to a single identity. Epigenetic mechanisms and tumor microenvironment cues push epithelial cells toward flexible, hybrid states that can shift into mesenchymal, neuroendocrine, or stem‐like fates, driving metastasis, drug resistance, and tumor heterogeneity. Targeting the epigenetic regulators behind these transitions, using
Ezgi Boyvatlı, Burcu Akman, Ezgi Bağırsakçı, Nese Atabey, Serap Erkek‐Ozhan   +4 more
wiley   +1 more source

Virtual Environment for Next Generation Sequencing Analysis [PDF]

, 2012
Next Generation Sequencing technology, on the one hand, allows a more accurate analysis, and, on the other hand, increases the amount of data to process.
Provenzano, R., Provenzano R., Terzo, Olivier, Mossucca, L., Acquaviva, Andrea, Mossucca L, Abate, Francesco   +6 more
core  

Direct RNA sequencing dataset of SMG1 KO mutant Physcomitrella (Physcomitrium patens)

Data in Brief, 2020
Nonsense-mediated mRNA decay (NMD) is a system that controls the quality of mRNA transcripts in eukaryotes by degradation of aberrant transcripts in a pioneer round of translation.
Andrey Knyazev, Anna Glushkevich, Igor Fesenko   +2 more
doaj   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

13C-direct detected NMR experiments for the sequential J-based resonance assignment of RNA oligonucleotides [PDF]

, 2010
We present here a set of 13C-direct detected NMR experiments to facilitate the resonance assignment of RNA oligonucleotides. Three experiments have been developed: (1) the (H)CC-TOCSY-experiment utilizing a virtual decoupling scheme to assign the ...
Helena Kovacs, Christian Richter, Anna Wacker, Buck, Janina, Kovacs, Helena, Wolfgang Bermel, Wacker, Anna, Harald Schwalbe, Bermel, Wolfgang, Boris Fürtig, Fürtig, Boris, Schwalbe, Harald (Prof. Dr.), Richter, Christian, Janina Buck   +13 more
core   +1 more source

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source