Results 101 to 110 of about 721,346 (281)

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Template-switching artifacts resemble alternative polyadenylation

BMC Genomics, 2019
Background Alternative polyadenylation is commonly examined using cDNA sequencing, which is known to be affected by template-switching artifacts. However, the effects of such template-switching artifacts on alternative polyadenylation are generally ...
Zsolt Balázs, Dóra Tombácz, Zsolt Csabai, Norbert Moldován, Michael Snyder, Zsolt Boldogkői   +5 more
doaj   +1 more source

Models for transcript quantification from RNA-Seq [PDF]

, 2011
RNA-Seq is rapidly becoming the standard technology for transcriptome analysis. Fundamental to many of the applications of RNA-Seq is the quantification problem, which is the accurate measurement of relative transcript abundances from the sequenced reads.
Pachter, Lior
core  

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

Common analysis of direct RNA sequencinG CUrrently leads to misidentification of m 5 C at GCU motifs

Life Science Alliance
Published predictions of 5-methylcytosine at GCU sites inferred from direct RNA sequencing and the Tombo Alternative Model should be reconsidered unless further validated with orthogonal methods.
Kaylee J Watson, Robin E Bromley, Benjamin C Sparklin, Mark T Gasser, Tamanash Bhattacharya, Jarrett F Lebov, Tyonna Tyson, Nan Dai, Laura E Teigen, Karen T Graf, Jeremy M Foster, Michelle Michalski, Vincent M Bruno, Amelia RI Lindsey, Ivan R Corrêa, Richard W Hardy, Irene LG Newton, Julie C Dunning Hotopp   +17 more
doaj   +1 more source

Alignment of the amino terminal amino acid sequence of human cytochrome c oxidase subunits I and II with the sequence of their putative mRNAs [PDF]

, 1981
Thirteen of the first fifteen amino acids from the NH2-terminus of the primary sequence of human cytochrome c oxidase subunit I and eleven of the first twelve amino acids of subunit II have been identified by microsequencing procedures.
Attardi, Giuseppe, Chomyn, Anne, Hunkapiller, Michael W.   +2 more
core   +2 more sources

AAA+ protein unfoldases—the Moirai of the proteome

FEBS Letters, EarlyView.
AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo‐electron microscopy (cryo‐EM) structures and single‐molecule biophysical data to reconcile competing models of substrate translocation.
Stavros Azinas, Marta Carroni
wiley   +1 more source

Evaluating the potential and limitations of nanopore adaptive sampling for targeted transcriptome sequencing

Genome Biology
Long-read RNA sequencing is a powerful technology for transcriptomics, but low throughput and high cost pose challenges. Adaptive sampling, a feature of Oxford Nanopore Technologies, offers real-time enrichment by selectively ejecting non-target ...
Nicole DeBruyne, Feng Wang, Yang Xu, Lan Lin   +3 more
doaj   +1 more source

Getting the Entire Message: Progress in Isoform Sequencing

Frontiers in Genetics, 2019
The advent of second-generation sequencing and its application to RNA sequencing have revolutionized the field of genomics by allowing quantification of gene expression, as well as the definition of transcription start/end sites, exons, splice sites and ...
Simon A. Hardwick, Simon A. Hardwick, Anoushka Joglekar, Paul Flicek, Adam Frankish, Hagen U. Tilgner   +5 more
doaj   +1 more source

A comprehensive functional map of the hepatitis C virus genome provides a resource for probing viral proteins. [PDF]

, 2014
UnlabelledPairing high-throughput sequencing technologies with high-throughput mutagenesis enables genome-wide investigations of pathogenic organisms. Knowledge of the specific functions of protein domains encoded by the genome of the hepatitis C virus ...
Arumugaswami, Vaithilingaraja, Chen, Shu-Hwa, Chen, Zugen, Chu, Virginia, Lin, Chung-Yen, Lo, Hung-Hao, Olson, C Anders, Qi, Hangfei, Remenyi, Roland, Stokelman, Tamar, Su, Sheng-Yao, Sun, Ren, Truong, Shawna, Wu, Nicholas C, Wu, Ting-Ting   +14 more
core   +2 more sources