Results 101 to 110 of about 4,264,667 (244)

Characterizing transcripts of HIV-1 different substrains using direct RNA sequencing

Heliyon
Post-transcriptional processing and modification of viral RNA, including alternative splicing, polyadenylation, and methylation, play crucial roles in regulating viral gene expression, enhancing genomic stability, and increasing replication efficiency ...
Weizhen Li, Yong Huang, Haowen Yuan, Jingwan Han, Zhengyang Li, Aiping Tong, Yating Li, Hanping Li, Yongjian Liu, Lei Jia, Xiaolin Wang, Jingyun Li, Bohan Zhang, Lin Li   +13 more
doaj   +1 more source

A context‐dependent modulatory role for eIF6 in acquired resistance to vemurafenib in melanoma

FEBS Letters, EarlyView.
Acquired resistance to vemurafenib upregulates the translation factor eIF6 in melanoma cells. Silencing eIF6 in resistant cells reduces proliferation and partially restores drug sensitivity, whereas its overexpression increases sensitivity across melanoma lines regardless of BRAF status, via modulation of mTOR, S6K, and MAPK signaling.
George Kyriakopoulos, Antonia Petropoulou, Alexandra Anastogianni, Angelina Bania, Vassilis Stamatakis, Argyris Alexiou, Chi‐Ping Day, Glenn Merlino, Katerina Grafanaki, Constantinos Stathopoulos   +9 more
wiley   +1 more source

Functional diversification of Argonautes in nematodes:an expanding universe [PDF]

, 2013
In the last decade, many diverse RNAi (RNA interference) pathways have been discovered that mediate gene silencing at epigenetic, transcriptional and post-transcriptional levels. The diversity of RNAi pathways is inherently linked to the evolution of Ago
Buck, Amy H, Blaxter, Mark
core   +1 more source

Evaluating the potential and limitations of nanopore adaptive sampling for targeted transcriptome sequencing

Genome Biology
Long-read RNA sequencing is a powerful technology for transcriptomics, but low throughput and high cost pose challenges. Adaptive sampling, a feature of Oxford Nanopore Technologies, offers real-time enrichment by selectively ejecting non-target ...
Nicole DeBruyne, Feng Wang, Yang Xu, Lan Lin   +3 more
doaj   +1 more source

Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research

FEBS Letters, EarlyView.
What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
wiley   +1 more source

RNA modification detection using direct RNA sequencing and nanoDoc2_4

, 2022
RNA modification detection using direct RNA sequencing and ...
openaire   +1 more source

Optimizing Splicing Junction Detection in Next Generation Sequencing Data on a Virtual-GRID Infrastructure [PDF]

, 2012
The new protocol for sequencing the messenger RNA in a cell, named RNA-seq produce millions of short sequence fragments. Next Generation Sequencing technology allows more accurate analysis but increase needs in term of computational resources. This paper
Provenzano, R., Provenzano R., Terzo, Olivier, Mossucca, L., Acquaviva, Andrea, Ficarra, Elisa, Mossucca L, Abate, Francesco   +7 more
core  

Geoseq: a tool for dissecting deep-sequencing datasets [PDF]

, 2010
Gurtowski J, Cancio A, Shah H, et al. Geoseq: a tool for dissecting deep-sequencing datasets. BMC Bioinformatics. 2010;11(1): 506.Background Datasets generated on deep-sequencing platforms have been deposited in various public repositories such as the ...
Gurtowski, James, Sachidanandam, Ravi, Robert Homann, Shah Hardik, Chaya Levovitz, Ravi Sachidanandam, Hardik Shah, James Gurtowski, Cancio, Anthony, Levovitz Chaya, George Ajish, Cancio Anthony, Homann, Robert, Anthony Cancio, Shah, Hardik, Homann Robert, Levovitz, Chaya, Sachidanandam Ravi, Ajish George, George, Ajish, Gurtowski James   +20 more
core   +1 more source

Nanopore direct RNA sequencing and the epitranscriptome: Advances in mapping native RNA landscapes

iMeta
Nanopore direct RNA sequencing (DRS) has transformed transcriptomics by enabling single‐molecule, long‐read sequencing of native RNA without the need for reverse transcription or amplification.
Tianyuan Zhang, Jia Li, Chao Tang, You Wu, Hao Wu, Xi‐Tong Zhu, Ziyang Luo, Hang Qin, Lishan Ding, Yu Zeng, Shiou Yih Lee, Xiaotao Shen, Shiwen Gao, Zhaoyang Tian, Qian Tang, Mian Li, Muhammad Tahir Ul Qamar, Yang Dong, Komivi Dossa, Yaxuan Zhang, Hu Chen, Sanqi An, Xiang Yu, Lu Chen, Dingjie Wang, Shengli Li, Ling‐Ling Chen, Yanqiang Li   +27 more
doaj   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source