Results 1 to 10 of about 105,876 (185)
The DNA (cytosine-5) methyltransferases [PDF]
Nucleic Acids Research, 1994 The m5C-MTases form a closely-knit family of enzymes in which common amino acid sequence motifs almost certainly translate into common structural and functional elements. These common elements are located predominantly in a single structural domain that performs the chemistry of the reaction.
Sanjay Kumar +6 more
semanticscholar +3 more sources
DNA bending induced by DNA (cytosine-5) methyltransferases. [PDF]
Nucleic Acids Research, 2000 DNA bending induced by six DNA (cytosine-5) methyltransferases was studied using circular permutation gel mobility shift assay. The following bend angles were obtained: M.BSP:RI (GG(m5)CC), 46-50 degrees; M.HAE:III (GG(m5)CC), 40-43 degrees; M.SIN:I (GGW(m5)CC), 34-37 degrees; M.SAU:96I (GGN(m5)CC), 52-57 degrees; M.HPA:II (C(m5)CGG), 30 degrees; and M.
T. Rasko, C. Finta, A. Kiss
semanticscholar +3 more sources
Enzymatic properties of de novo-type mouse DNA (cytosine-5) methyltransferases. [PDF]
Nucleic Acids Research, 2001 We have purified GST-fused recombinant mouse Dnmt3a and three isoforms of mouse Dnmt3b to near homogeneity. Dnmt3b3, an isoform of Dnmt3b, did not have DNA methylation activity. Dnmt3a, Dnmt3b1 or Dnmt3b2 showed similar activity toward poly(dG-dC)-poly(dG-dC) for measuring de novo methylation activity, and toward poly(dI-dC)-poly(dI-dC) for measuring ...
Asako Aoki +6 more
semanticscholar +3 more sources
Engineering the DNA cytosine-5 methyltransferase reaction for sequence-specific labeling of DNA [PDF]
Nucleic Acids Research, 2012 DNA methyltransferases catalyse the transfer of a methyl group from the ubiquitous cofactor S-adenosyl-L-methionine (AdoMet) onto specific target sites on DNA and play important roles in organisms from bacteria to humans.
G. Lukinavičius +4 more
semanticscholar +5 more sources
Molecular Cloning, Expression, and Characterization of Starfish DNA (Cytosine-5)-methyltransferases
Bioscience, Biotechnology, and Biochemistry, 2012 To determine whether and if so how a DNA methylation-dependent epigenetic mechanism for transcriptional gene silencing functions in Echinoderms, we cloned and sequenced dnmt1 and dnmt3 cDNAs of the starfish Asterina pectinifera. Since the Strongylocentrotus purpuratus genome has only two loci of DNA (cytosine-5)-methyltransferase genes encoding Dnmt1 ...
Y. Fujihara +4 more
semanticscholar +3 more sources
Plant cytosine-5 DNA methyltransferases: structure, function, and molecular evolution.
Genomics, 2007 A detailed analysis of the structure and function, along with evolutionary aspects, of the main plant cytosine-5 DNA methyltransferases (C5-MTases) is presented. The evolutionary relationships between the already known and four candidate plant C5-MTases identified in this work were investigated using the distance, maximum-parsimony, and maximum ...
Athanasia Pavlopoulou, S. Kossida
semanticscholar +4 more sources
Diverse Domains of (Cytosine-5)-DNA Methyltransferases: Structural and Functional Characterization [PDF]
, 2012 (Cytosine-5)-DNA methyltransferases (C5-DNA MTases) are enzymes which catalyze meth‐ yl group transfer from S-adenosyl-L-methionine (AdoMet) to C5 atom of cytosine residue in DNA.
A. Ryazanova +3 more
semanticscholar +4 more sources
The Impact of the HydroxyMethylCytosine epigenetic signature on DNA structure and function
PLoS Computational Biology, 2021 We present a comprehensive, experimental and theoretical study of the impact of 5-hydroxymethylation of DNA cytosine. Using molecular dynamics, biophysical experiments and NMR spectroscopy, we found that Ten-Eleven translocation (TET) dioxygenases ...
Federica Battistini +9 more
doaj +2 more sources
bioRxiv, 2022 Background DNA methyltransferases (MTases) are enzymes that induce methylation, one of the representative epigenetic modifications of DNA, and are also useful tools for analyzing epigenomes.
F. Miura +8 more
semanticscholar +1 more source
The behaviour of 5-hydroxymethylcytosine in bisulfite sequencing. [PDF]
PLoS ONE, 2010 We recently showed that enzymes of the TET family convert 5-mC to 5-hydroxymethylcytosine (5-hmC) in DNA. 5-hmC is present at high levels in embryonic stem cells and Purkinje neurons.
Yun Huang +5 more
doaj +1 more source