Results 221 to 230 of about 94,067 (257)

Recognition of unusual DNA structures by human DNA(cytosine-5)methyltransferase

Journal of Molecular Biology, 1991
The symmetry of the responses of the human DNA (cytosine-5)methyltransferase to alternative placements of 5-methylcytosine in model oligodeoxynucleotide duplexes containing unusual structures has been examined. The results of these experiments more clearly define the DNA recognition specificity of the enzyme. A simple three-nucleotide recognition motif
S S, Smith, J L, Kan, D J, Baker, B E, Kaplan, P, Dembek   +4 more
openaire   +2 more sources

Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases

Nature Genetics, 1998
Cloning and characterization of a family of novel mammalian DNA (cytosine-5 ...
Masaki Okano, Okano Masaki, Li En
exaly   +3 more sources

The study of the interaction of (cytosine-5)-DNA methyltransferase SsoII with DNA by acoustic method

The Analyst, 2011
The interaction of (cytosine-5)-DNA methyltransferase SsoII (M.SsoII) with double-stranded DNA was studied by means of thickness shear mode acoustic method (TSM) and gel electrophoresis. M.SsoII recognizes in double-stranded DNA the methylation site 5'-CCNGG-3' (N=A, C, G, T) and methylates the inner cytosine residue.
A Yu, Ryazanova, E A, Kubareva, I, Grman, N V, Lavrova, E M, Ryazanova, T S, Oretskaya, T, Hianik   +6 more
openaire   +2 more sources

Human DNA (cytosine-5)-methyltransferases: a functional and structural perspective for epigenetic cancer therapy

Biochimie, 2017
Epigenetic modifications modulate chromatin states to regulate gene expression. Among them, DNA methylation and histone modifications play a crucial role in the establishment of the epigenome. In cancer, these epigenetic events may act in concert to repress tumor suppressor genes or promote oncogenic pathways.
Rondelet, Grégoire, Wouters, Johan
openaire   +2 more sources

Isolation and characterisation of DNA cytosine 5-methyltransferase from human placenta

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1983
DNA cytosine 5-methyltransferase has been extensively purified (about 2600-fold) from the soft tissue of human placenta by chromatography on DEAE-cellulose and hydroxyapatite, and by an affinity step on agarose-immobilized S-adenosylhomocysteine. The isolated enzyme has a molecular weight of 135,000 and methylates DNA from various sources in native and
G P, Pfeifer, S, Grünwald, T L, Boehm, D, Drahovsky   +3 more
openaire   +2 more sources

Direct identification of the active-site nucleophile in a DNA (cytosine-5)-methyltransferase

Biochemistry, 1991
The overproduction, purification, and determination of the active-site catalytic nucleophile of the DNA (cytosine-5)-methyltransferase (DCMtase) enzyme M.HaeIII are reported. Incubation of purified M.HaeIII with an oligodeoxynucleotide specifically modified with the mechanism-based inhibitor 5-fluoro-2'-deoxycytidine [Osterman, D. G., et al.
L, Chen, A M, MacMillan, W, Chang, K, Ezaz-Nikpay, W S, Lane, G L, Verdine   +5 more
openaire   +2 more sources

The influence of the dT·dG mispair on the activity of the human DNA(cytosine-5)methyltransferase

Biochemical and Biophysical Research Communications, 1987
Synthetic oligodeoxynucleotides containing a dT.dG mispair at a centrally located d(pCG) dimer are methylated at a moderate rate by highly purified human DNA(cytosine-5)methyltransferase (E.C. 2.1.1.37). The presence of a mispaired dT in one strand induced the enzyme to preferentially methylate the opposite strand.
D J, Baker, T A, Hardy, S S, Smith
openaire   +2 more sources

Influence of photoperiod on expression of DNA (cytosine-5) methyltransferases in Atlantic cod

Gene, 2013
Photoperiod manipulation during early juvenile stages can influence growth in Atlantic cod. In the present study, one group of cod juveniles were reared under natural photoperiod conditions for Bodø (67° N, 14° E), whereas their counterparts were kept under continuous illumination.
GIANNETTO, alessia, Kazue Nagasawa, FASULO, Salvatore, Jorge M. O. Fernandes   +3 more
openaire   +3 more sources

[Properties of DNA-(cytosine-5-)-methyltransferase in the brain].

Comptes rendus des seances de la Societe de biologie et de ses filiales, 1986
Beef brain DNA-(cytosine-5-)-methyltransferase was partially purified by chromatography on Ultrogel AcA34 and Dyematrex Blue A. The purification was of 360 times and the recovery of 75%. The pH optimum of the reaction is 7.6 NaCl inhibits double stranded DNA methylation, but stimulates single stranded DNA methylation up to 50 mM, before inhibiting ...
A, Pfohl-Leszkowicz, G, Keith, G, Dirheimer   +2 more
openaire   +1 more source

Impact of Cytosine 5-Halogens on the Interaction of DNA with Restriction Endonucleases and Methyltransferase

Chemical Research in Toxicology, 2006
Growing evidence from both prokaryotes and eukaryotes indicates that pyrimidine 5-methyl groups can have profound biological consequences that are mediated by the affinity of DNA-protein interactions. The presence of the 5-methyl group could potentially create a steric block preventing the binding of some proteins whereas the affinity of many other ...
Victoria, Valinluck, Winnie, Wu, Pingfang, Liu, Jonathan W, Neidigh, Lawrence C, Sowers   +4 more
openaire   +2 more sources