Results 41 to 50 of about 55,108 (306)

The nonhomologous end-joining pathway of DNA repair is required for genomic stability and the suppression of translocations [PDF]

Proceedings of the National Academy of Sciences, 2000
We have used spectral karyotyping to assess potential roles of three different components of the nonhomologous DNA end-joining pathway in the maintenance of genomic stability in mouse embryonic fibroblasts (MEFs). MEFs homozygous for mutations that inactivate either DNA ligase IV (Lig4) or Ku70 display dramatic genomic instability, even in the absence ...
D O, Ferguson, J M, Sekiguchi, S, Chang, K M, Frank, Y, Gao, R A, DePinho, F W, Alt   +6 more
openaire   +2 more sources

Chromosomal instability syndromes are sensitive to poly ADP-ribose polymerase inhibitors

Haematologica, 2008
Poly ADP-ribose polymerase inhibitors have been shown to target cells with homologous recombination DNA repair defects. We report that poly ADP-ribose polymerase inhibitors induces apoptosis in cells deficient in other key DNA repair components ...
Terry J. Gaymes, Sydney Shall, Farzin Farzaneh, Ghulam J. Mufti   +3 more
doaj   +1 more source

Impact of DNA ligase IV on nonhomologous end joining pathways during class switch recombination in human cells

, 2005
Class switch recombination (CSR) is a region-specific, transcriptionally regulated, nonhomologous recombinational process that is initiated by activation-induced cytidine deaminase (AID).
Lennart Hammarström, Hammarström, L,, Lahdesmaki, A, Aleksi Lähdesmäki, Wei Zhou, Andrew R. Gennery, Jones, AM, Michael R. Ehrenstein, Pan-Hammarstrom, Q, Hammarstrom, L, Zhou, W, Gennery, AR, Ehrenstein, MR, Anne-Marie Jones, Richard A. Gatti, Gatti, RA, Qiang Pan-Hammarström   +16 more
core   +1 more source

Recruitment kinetics of DNA repair proteins Mdc1 and Rad52 but not 53BP1 depend on damage complexity. [PDF]

, 2012
The recruitment kinetics of double-strand break (DSB) signaling and repair proteins Mdc1, 53BP1 and Rad52 into radiation-induced foci was studied by live-cell fluorescence microscopy after ion microirradiation.
Hilmar Strickfaden (148181), Guenther Dollinger, Brüning, Tino, Anja Derer, Judith Seel (148178), Volker Hable, Derer, Anja, Guido A Drexler, Burgdorf, Christian, Tino Brüning, Drexler, Guido A., Dollinger, Günther, Friedl, Anna A., Christoph Greubel, Greubel, Christoph, Thomas Cremer, Christian Burgdorf, Guido A. Drexler (148161), Christian Burgdorf (148169), Hable, Volker, Tino Brüning (148165), Hilmar Strickfaden, Judith Seel, Christoph Greubel (148173), Thomas Cremer (93), Günther Dollinger (148186), Anna A Friedl, Cremer, Thomas, Guido A. Drexler, Anna A. Friedl, Anja Derer (148175), Strickfaden, Hilmar, Günther Dollinger, Anna A. Friedl (148183), Seel, Judith, Volker Hable (148157)   +35 more
core   +1 more source

A comparison of DNA repair pathways to achieve a site-specific gene modification of the Bruton's tyrosine kinase gene

Molecular Therapy: Nucleic Acids, 2022
Gene editing utilizing homology-directed repair has advanced significantly for many monogenic diseases of the hematopoietic system in recent years but has also been hindered by decreases between in vitro and in vivo gene integration rates.
David H. Gray, Jasmine Santos, Alexandra Grace Keir, Isaac Villegas, Simon Maddock, Edward C. Trope, Joseph D. Long, Caroline Y. Kuo   +7 more
doaj   +1 more source

Structure of the Helicase Domain of DNA Polymerase Theta Reveals a Possible Role in the Microhomology-Mediated End-Joining Pathway

, 2015
DNA polymerase theta (Polθ) has been identified as a crucial alternative non-homologous end-joining factor in mammalian cells. Polθ is upregulated in a range of cancer cell types defective in homologous recombination, and knockdown has been shown to ...
Aitkenhead, H, Gileadi, Opher, Cooper, Christopher D O, Newman, JA, Cooper, CD, Gileadi, O, Cooper, Christopher D.O., Newman, Joseph A., Aitkenhead, Hazel   +8 more
core   +1 more source

CometChip enables parallel analysis of multiple DNA repair activities

, 2021
DNA damage can be cytotoxic and mutagenic, and it is directly linked to aging, cancer, and other diseases. To counteract the deleterious effects of DNA damage, cells have evolved highly conserved DNA repair pathways.
Kay, Jennifer E, David M. Weingeist, Elina Thadhani, Leona D. Samson, Simran Kaushal, Jennifer E. Kay, Sobol, Robert W, Bevin P. Engelward, Ge, Jing, Kaushal, Simran, Le P. Ngo, Jessica L. Fessler, Chow, Danielle N, Patrizia Mazzucato, Samson, Leona D, Floyd, Scott R, Danielle N. Chow, Scott R. Floyd, Mazzucato, Patrizia, Ngo, Le P, Jing Ge, Fessler, Jessica L, Tay, Ian J, Thadhani, Elina, Robert W. Sobol, Weingeist, David M, Ian J. Tay, Engelward, Bevin P   +27 more
core   +1 more source

Protocol: A Multiplexed Reporter Assay to Study Effects of Chromatin Context on DNA Double-Strand Break Repair

Frontiers in Genetics, 2022
DNA double-strand breaks (DSBs) can be repaired through various pathways. Understanding how these pathways are regulated is of great interest for cancer research and optimization of gene editing.
Ruben Schep, Christ Leemans, Eva K. Brinkman, Tom van Schaik, Bas van Steensel, Bas van Steensel   +5 more
doaj   +1 more source

XLF/Cernunnos: An important but puzzling participant in the nonhomologous end joining DNA repair pathway [PDF]

DNA Repair, 2017
DNA double strand breaks (DSBs) are one of the most deleterious DNA lesions that promote cell death, genomic instability and carcinogenesis. The two major cellular mechanisms that repair DSBs are Nonhomologous End-Joining (NHEJ) and Homologous Recombination Repair (HRR).
Vijay, Menon, Lawrence F, Povirk
openaire   +2 more sources

The ubiquitin‐proteasome system and autophagy as guardians of the cellular proteome

FEBS Letters, EarlyView.
This Perspective covers the three principles governing the crosstalk between the ubiquitin‐proteasome system and autophagy in cellular proteostasis: (1) a shared ubiquitin code routing substrates via shuttle factors or autophagy receptors; (2) spatial compartmentalization into phase‐separated degradation hubs and organelle‐specific modules (exemplified
Ivan Dikic
wiley   +1 more source