Results 101 to 110 of about 678 (148)
Programmable DNA pyrimidine base editing via engineered uracil-DNA glycosylase
Nature CommunicationsDNA base editing technologies predominantly utilize engineered deaminases, limiting their ability to edit thymine and guanine directly. In this study, we successfully achieve base editing of both cytidine and thymine by leveraging the translesion DNA ...
Zongyi Yi +10 more
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BiomoleculesEukaryotic genomic DNA is packaged into chromatin as nucleosomes, where it remains susceptible to reactive oxygen species (ROS) that generate the mutagenic lesion 8-oxo-7,8-dihydroguanine (8oxoG).
Abigayle F. Vito +12 more
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Nature CommunicationsDNA base editors enable direct editing of adenine (A), cytosine (C), or guanine (G), but there is no base editor for direct thymine (T) editing currently. Here we develop two deaminase-free glycosylase-based base editors for direct T editing (gTBE) and C
Huawei Tong +15 more
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Unified catalytic mechanism for DNA glycosylases
Journal of Biological Chemistry, 1994 M L, Dodson, M L, Michaels, R S, Lloyd
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DNA glycosylase recognition and catalysis
Current Opinion in Structural Biology, 2004DNA glycosylases are the enzymes responsible for recognizing base lesions in the genome and initiating base excision DNA repair. Recent structural and biochemical results have provided novel insights into DNA damage recognition and repair. The basis of the recognition of the oxidative lesion 8-oxoguanine by two structurally unrelated DNA glycosylases ...
Gregory Verdine, J Christopher Fromme
exaly +3 more sources
Processivity of uracil DNA glycosylase
Mutation Research DNA Repair, 1993The purpose of this study was to determine the mechanism by which uracil DNA glycosylase locates uracil residues within double-stranded DNA. Using reaction conditions that contained low salt concentrations, the addition of uracil DNA glycosylase to plasmid DNAs containing multiple, randomly incorporated uracils resulted in the accumulation of form III ...
R Stephen Lloyd, R S Lloyd
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Molecular and Cellular Biochemistry, 1982
Various DNA glycosylases exist, which initiate the first step in base-excision repair. A summary of the kinetic and physical characteristics of three classes of DNA glycosylases are presented here. The first class discussed, include glycosylases which recognize alkylated DNA.
S J, Caradonna, Y C, Cheng
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Various DNA glycosylases exist, which initiate the first step in base-excision repair. A summary of the kinetic and physical characteristics of three classes of DNA glycosylases are presented here. The first class discussed, include glycosylases which recognize alkylated DNA.
S J, Caradonna, Y C, Cheng
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Structures and functions of DNA glycosylases
Mutation Research/DNA Repair, 1990Treatment of cells with a methylating agent, such as methyl methanesulfonate (MMS) and Nmethyl-N-nitrosourea (MNU), yields various methylated bases in DNA (Riazuddin and Lindahl, 1978). Organisms possess mechanisms to repair these methylated bases, which are potentially harmful to their genetic material.
K, Sakumi, M, Sekiguchi
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The enigmatic thymine DNA glycosylase
DNA Repair, 2007When it was first isolated from extracts of HeLa cells in Josef Jiricny's laboratory, the thymine DNA glycosylase (TDG) attracted attention because of its ability to remove thymine, i.e. a normal DNA base, from G.T mispairs. This implicated a function of DNA base excision repair in the restoration of G.C base pairs following the deamination of a 5 ...
Cortázar, Daniel +4 more
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