Results 81 to 90 of about 43,780 (231)
Journal of Integrative Plant Biology, EarlyView.Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang +7 more
wiley +1 more source
Structure of Formamidopyrimidine-DNA Glycosylase Covalently Complexed to DNA [PDF]
Journal of Biological Chemistry, 2002 Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme that excises oxidized purines from damaged DNA. The Schiff base intermediate formed during this reaction between Escherichia coli Fpg and DNA was trapped by reduction with sodium borohydride, and the structure of the resulting covalently cross-linked complex was determined at a 2.1-A ...
Rotem, Gilboa +8 more
openaire +2 more sources
Enhancing CRISPR‐Cas12a base editing in plants with LbCas12a variants and introns
Journal of Integrative Plant Biology, EarlyView.Intron optimization of LbCas12a‐RRV improves cytosine and adenine base editing efficiency in plants, supports multiplexed and double‐strand break‐free genome modification, and expands precise genome engineering tools for crop breeding and plant functional research. ABSTRACT Cytosine base editors (CBEs) and adenine base editors (ABEs) are powerful tools
Yanhao Cheng +5 more
wiley +1 more source
Frontiers in Cell and Developmental Biology, 2020 Human telomeres as well as more than 40% of human genes near the promoter regions have been found to contain the sequence that may form a G-quadruplex structure.
Alexandra A. Kuznetsova +4 more
doaj +1 more source
The Emerging Role of Ten-Eleven Translocation 1 in Epigenetic Responses to Environmental Exposures. [PDF]
, 2020 Mounting evidence from epidemiological studies and animal models has linked exposures to environmental factors to changes in epigenetic markers, especially in DNA methylation.
Brown, Anthony P, Ji, Hong, Zhu, Tao
core
7,8-Dihydro-8-oxoadenine, a highly mutagenic adduct, is repaired by Escherichia coli and human mismatch-specific uracil/thymine-DNA glycosylases [PDF]
, 2012 Hydroxyl radicals predominantly react with the C(8) of purines forming 7,8-dihydro-8-oxoguanine (8oxoG) and 7,8-dihydro-8-oxoadenine (8oxoA) adducts, which are highly mutagenic in mammalian cells.
Couve, Sophie +5 more
core +2 more sources
Uracil-DNA Glycosylase UNG Promotes Tet-mediated DNA Demethylation [PDF]
Journal of Biological Chemistry, 2016 In mammals, active DNA demethylation involves oxidation of 5-methylcytosine (5mC) into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by Tet dioxygenases and excision of these two oxidized bases by thymine DNA glycosylase (TDG). Although TDG is essential for active demethylation in embryonic stem cells and induced pluripotent stem cells, it is ...
Jian-Huang, Xue +9 more
openaire +2 more sources
Journal of Integrative Plant Biology, EarlyView.Two optimized TadA‐derived base editors efficiently generate diverse splicing variants by targeting specific splice sites in potato. ABSTRACT Pre‐messenger RNA (pre‐mRNA) splicing is a critical mechanism for post‐transcriptional regulation in plants. Through alternative splicing, plants produce diverse transcriptomes and proteomes that finely regulate ...
Kaiyuan Chen +8 more
wiley +1 more source
Nature Communications, 2020 DNA glycosylases are lesion-specific enzymes that recognize specific nucleobase damages and catalyze their excision through cleavage of the glycosidic bond.
Uddhav K. Shigdel +6 more
doaj +1 more source
Expression and the Peculiar Enzymatic Behavior of the Trypanosoma cruzi NTH1 DNA Glycosylase. [PDF]
PLoS ONE, 2016 Trypanosoma cruzi, the etiological agent of Chagas' disease, presents three cellular forms (trypomastigotes, epimastigotes and amastigotes), all of which are submitted to oxidative species in its hosts. However, T.
Fernando Ormeño +10 more
doaj +1 more source