Functional Traits Co-Occurring with Mobile Genetic Elements in the Microbiome of the Atacama Desert [PDF]
, 2019 Mobile genetic elements (MGEs) play an essential role in bacterial adaptation and evolution. These elements are enriched within bacterial communities from extreme environments. However, very little is known if specific genes co-occur with MGEs in extreme
Airo, Alessandro +4 more
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ATP hydrolysis by the SNF2 domain of the ultraspecific maintenance methylase Dnmt5 drives recognition and modification of hemimethylated DNA [PDF]
, 2020 SUMMARYC. neoformansDnmt5 is an ultraspecific maintenance-type CpG methyltransferase (DNMT) that mediates long-term epigenome evolution. It harbors a DNMT domain and SNF2 ATPase domain. We find that the SNF2 domain couples substrate specificity to an ATPase step that is essential for DNA methylation.
Dumesic, Phillip A. +4 more
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Infection with a Virulent Strain of Wolbachia Disrupts Genome Wide-Patterns of Cytosine Methylation in the Mosquito Aedes aegypti [PDF]
, 2015 BACKGROUND Cytosine methylation is one of several reversible epigenetic modifications of DNA that allow a greater flexibility in the relationship between genotype and phenotype.
Caragata, Eric P. +7 more
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Introduction and expression of the bacterial PaeR7 methylase gene in mammalian cells [PDF]
, 1986 An approach is devised for studying the role of DNA methylation in eukaryotic gene expression. The approach is based on the expression of site-specific bacterial methylase genes in animal cells.
Kwoh, T. J. +5 more
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Purification and characterization of the MspI DNA methyltransferase cloned and overexpressed in E. coli [PDF]
, 1992 The MspI restriction-modification system, which recognizes the sequence 5'-CCGG-3', has been previously cloned and sequenced (1). We subcloned the methyltransferase gene (M.MspI) downstream of the ptac promoter in the multicopy vector pUC119 and ...
Dubey, A. K. +2 more
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Journal of Biological Chemistry, 1991 A DNA fragment of about 3.4 kilobase pairs that expressed the HgaI modification activity was cloned from the chromosomal DNA of Haemophilus gallinarum, and its nucleotide sequence was determined. Two open reading frames (ORF) which could code for structurally similar proteins were identified in the upstream and middle regions and a truncated ORF in the
H, Sugisaki, K, Yamamoto, M, Takanami
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Cloning and characterization of the genes encoding the MspI restriction modification system [PDF]
, 1989 The genes encoding the MspI restriction modification system, which recognizes the sequence 5' CCGG, have been cloned into pUC9. Selection was based on expression of the cloned methylase gene which renders plasmid DNA insensitive to MspI cleavage in vitro.
Lin, P. M. +2 more
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The enzymes LSD1 and Set1A cooperate with the viral protein HBx to establish an active hepatitis B viral chromatin state [PDF]
, 2016 Indexación: Web of ScienceWith about 350 million people chronically infected around the world hepatitis B is a major health problem. Template for progeny HBV synthesis is the viral genome, organized as a minichromosome (cccDNA) inside the hepatocyte ...
Alarcon, V +9 more
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NF-κB: Regulation by Methylation [PDF]
, 2015 In normal cells exposed to stress, the central transcription factor NF-κB is activated only transiently, to modulate the activation of downstream immune responses.
Lu, Tao, Stark, George R.
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Comparative (computational) analysis of the DNA methylation status of trinucleotide repeat expansion diseases [PDF]
, 2013 Copyright © 2013 Mohammadmersad Ghorbani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is ...
Ghorbani, M +3 more
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