Results 1 to 10 of about 236,210 (159)
Structures of the type I DNA restriction enzymes. [PDF]
Proc Natl Acad Sci U S A, 2017 The article by Liu et al. (1) on the structure of type I DNA restriction and modification enzymes purports to significantly advance our understanding of these enzymes and proposes a model for their operation. While the partial structure of one of these enzymes is interesting and defines the interface between some of the subunits, the article contains ...
Dryden DTF.
europepmc +4 more sources
NEBcutter: a program to cleave DNA with restriction enzymes [PDF]
Nucleic Acids Research, 2003 NEBcutter, version 1.0, is a program available via a web server (http://tools.neb.com/NEBcutter) that will accept an input DNA sequence and produce a comprehensive report of the restriction enzymes that will cleave the sequence. It produces a variety of outputs including restriction enzyme maps, theoretical digests and links into the restriction enzyme
Richard Roberts +2 more
exaly +4 more sources
REBASE—enzymes and genes for DNA restriction and modification [PDF]
Nucleic Acids Research, 2007 REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in the biological process of restriction-modification. It contains fully referenced information about recognition and cleavage sites, isoschizomers, neoschizomers, commercial availability, methylation sensitivity, crystal ...
Richard Roberts, Tamás Vincze, J Posfai
exaly +3 more sources
The interplay between supercoiling and DNA modifying enzymes at the single-molecule level [PDF]
Scientific ReportsDNA supercoiling refers to the overwinding or underwinding of the double helix, forming plait-like structures called plectonemes. Supercoiling is involved in all biological processes that occur on DNA.
Elise M. Wilkinson +4 more
doaj +2 more sources
A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes [PDF]
Nucleic Acids Research, 2003 A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many different Type II enzymes now identified and provides a system for naming the putative genes found by sequence analysis of microbial genomes.
Barry L Stoddard +2 more
exaly +8 more sources
Structure and operation of the DNA-translocating type I DNA restriction enzymes. [PDF]
Genes Dev, 2012 Type I DNA restriction/modification (RM) enzymes are molecular machines found in the majority of bacterial species. Their early discovery paved the way for the development of genetic engineering. They control (restrict) the influx of foreign DNA via horizontal gene transfer into the bacterium while maintaining sequence-specific methylation ...
Kennaway CK +15 more
europepmc +6 more sources
Type II Restriction of 2-Aminoadenosine (dZ)-Modified DNA and Production of dZ-Modified Plasmid in E. coli [PDF]
VirusesThe modified DNA base 2,6 aminopurine (2-aminoadenine, (d)Z base) was originally found in phages to counteract host-encoded restriction systems. However, only a limited number of restriction endonucleases (REases) have been tested on dZ-modified DNA ...
Weiwei Yang +5 more
doaj +2 more sources
Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering
Fermentation, 2023 Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
doaj +1 more source
REBASE--restriction enzymes and DNA methyltransferases [PDF]
Nucleic Acids Research, 2004 REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in restriction-modification. It contains both published and unpublished work with information about recognition and cleavage sites, isoschizomers, commercial availability, crystal and sequence data.
Richard J. Roberts +3 more
openaire +2 more sources
Restriction enzyme digestion of hemimethylated DNA [PDF]
Nucleic Acids Research, 1981 Hemimethylated duplex DNA of the bacteriophage phi X 174 was synthesized using primed repair synthesis is in vitro with E. coli DNA polymerase I followed by ligation to produce the covalently closed circular duplex (RFI). Single-stranded phi X DNA was used as a template, a synthetic oligonucleotide as primer and 5-methyldeoxycytidine-5'-triphosphate ...
Y, Gruenbaum, H, Cedar, A, Razin
openaire +2 more sources