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Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering

open access: yesFermentation, 2023
Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
doaj   +1 more source

Use Cutting Enzymes to Encode the Secret Message [PDF]

open access: yesAl-Rafidain Journal of Computer Sciences and Mathematics, 2014
Researchers begin to explore new methods to manage the huge amount of data shared over the Internet, in order to use them more efficiently and safely, One of the ways that is raised nowadays is Steganography, The art of information hiding within ...
Saadoun A.
doaj   +1 more source

In vitro Type II Restriction of Bacteriophage DNA With Modified Pyrimidines

open access: yesFrontiers in Microbiology, 2020
To counteract host-encoded restriction systems, bacteriophages (phages) incorporate modified bases in their genomes. For example, phages carry in their genomes modified pyrimidines such as 5-hydroxymethyl-cytosine (5hmC) in T4gt deficient in α- and β ...
Kiersten Flodman   +4 more
doaj   +1 more source

Revised selection criteria for candidate restriction enzymes in genome walking. [PDF]

open access: yesPLoS ONE, 2012
A new method to improve the efficiency of flanking sequence identification by genome walking was developed based on an expanded, sequential list of criteria for selecting candidate enzymes, plus several other optimization steps.
Ali Taheri   +3 more
doaj   +1 more source

DNA polymorphism investigation among local Brucella isolates [PDF]

open access: yesIraqi Journal of Veterinary Sciences, 2009
The present study was undertaken to investigate the deoxyribonucleic acid (DNA) polymorphism finger printing among DNA of local Brucella isolates which is a new method in molecular epidemiological studies.
S. A. AL-Bayatti ¹ and A. N. Al-Thwani ²
doaj   +1 more source

A physical map of human Alu repeats cleavage by restriction endonucleases

open access: yesBMC Genomics, 2008
Background Alu repetitive elements are the abundant sequences in human genome. Diversity of DNA sequences of these elements makes difficult the construction of theoretical patterns of Alu repeats cleavage by restriction endonucleases.
Chernukhin Valery A   +3 more
doaj   +1 more source

Fused eco29kIR- and M genes coding for a fully functional hybrid polypeptide as a model of molecular evolution of restriction-modification systems

open access: yesBMC Evolutionary Biology, 2011
Background The discovery of restriction endonucleases and modification DNA methyltransferases, key instruments of genetic engineering, opened a new era of molecular biology through development of the recombinant DNA technology.
Solonin Alexander S   +2 more
doaj   +1 more source

Universal Linker and Ligation Procedures for Construction of Genomic DNA Libraries Enriched for Microsatellites

open access: yesBioTechniques, 1999
Microsatellite loci are highly informative genetic markers useful for population genetic studies, linkage mapping and parentage determination. Methods to identify novel microsatellite loci commonly use subtractive hybridization to enrich smallinsert ...
Matthew B. Hamilton   +3 more
doaj   +1 more source

Restriction Fragment Length Polymorphism Linkage Map for Arabidopsis thaliana [PDF]

open access: yes, 1988
We have constructed a restriction fragment length polymorphism linkage map for the nuclear genome of the flowering plant Arabidopsis thaliana. The map, containing 90 randomly distributed molecular markers, is physically very dense; >50% of the genome is ...
Bowman, John L.   +4 more
core   +1 more source

Biomolecular computers with multiple restriction enzymes

open access: yesGenetics and Molecular Biology, 2017
The development of conventional, silicon-based computers has several limitations, including some related to the Heisenberg uncertainty principle and the von Neumann “bottleneck”.
Sebastian Sakowski   +5 more
doaj   +1 more source

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