Re-evaluating the kinetics of ATP hydrolysis during initiation of DNA sliding by Type III restriction enzymes [PDF]
, 2015 DNA cleavage by the Type III restriction enzymes requires long-range protein communication between recognition sites facilitated by thermally-driven 1D diffusion. This ‘DNA sliding’ is initiated by hydrolysis of multiple ATPs catalysed by a helicase-like
Bollins, Jack M +2 more
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MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme [PDF]
Nucleic Acids Research, 2018 Efficient DNA assembly is of great value in biological research and biotechnology. Type IIS restriction enzyme-based assembly systems allow assembly of multiple DNA fragments in a one-pot reaction.
Da Lin, Christopher A. O’Callaghan
openalex +2 more sources
Structures of the type I DNA restriction enzymes. [PDF]
Proc Natl Acad Sci U S A, 2017 Dryden DTF.
europepmc +2 more sources
Open Biology, 2015 Characterization of dynamic DNA methylomes in diverse phylogenetic groups has attracted growing interest for a better understanding of the evolution of DNA methylation as well as its function and biological significance in eukaryotes.
Shi Wang +10 more
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Restriction enzymes use a 24 dimensional coding space to recognize 6 base long DNA sequences. [PDF]
PLoS ONE, 2019 Restriction enzymes recognize and bind to specific sequences on invading bacteriophage DNA. Like a key in a lock, these proteins require many contacts to specify the correct DNA sequence.
Thomas D Schneider, Vishnu Jejjala
doaj +2 more sources
Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes
PLoS ONE, 2009 We have developed a protocol to assemble in one step and one tube at least nine separate DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained containing the desired construct.
Carola Engler +3 more
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DNA translocation blockage, a general mechanism of cleavage site selection by type I restriction enzymes [PDF]
EMBO Journal, 1999 Pavel Janščák +4 more
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Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering
Fermentation, 2023 Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
doaj +1 more source
A prediction of the amino acids and structures involved in DNA recognition by type I DNA restriction and modification enzymes [PDF]
Nucleic Acids Research, 1997 Simon Sturrock
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REBASE: a database for DNA restriction and modification: enzymes, genes and genomes
Nucleic Acids Res., 2022 REBASE is a comprehensive and extensively curated database of information about the components of restriction-modification (RM) systems. It is fully referenced and provides information about the recognition and cleavage sites for both restriction enzymes
R. Roberts +3 more
semanticscholar +1 more source