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Structures of the type I DNA restriction enzymes. [PDF]
Proc Natl Acad Sci U S A, 2017 The article by Liu et al. (1) on the structure of type I DNA restriction and modification enzymes purports to significantly advance our understanding of these enzymes and proposes a model for their operation. While the partial structure of one of these enzymes is interesting and defines the interface between some of the subunits, the article contains ...
Dryden DTF.
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Site-specific DNA transesterification catalyzed by a restriction enzyme [PDF]
Proceedings of the National Academy of Sciences, 2007 Most restriction endonucleases use Mg 2+ to hydrolyze phosphodiester bonds at specific DNA sites. We show here that BfiI, a metal-independent restriction enzyme from the phospholipase D superfamily, catalyzes both DNA hydrolysis and transesterification reactions at its recognition site.
Sasnauskas, G +3 more
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Restriction enzymes use a 24 dimensional coding space to recognize 6 base long DNA sequences. [PDF]
PLoS ONE, 2019 Restriction enzymes recognize and bind to specific sequences on invading bacteriophage DNA. Like a key in a lock, these proteins require many contacts to specify the correct DNA sequence.
Thomas D Schneider, Vishnu Jejjala
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Restriction Enzyme BsoBI–DNA Complex [PDF]
Structure, 2001 van der Woerd, Mark J +3 more
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Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering
Fermentation, 2023 Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
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REBASE--restriction enzymes and DNA methyltransferases [PDF]
Nucleic Acids Research, 2004 REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in restriction-modification. It contains both published and unpublished work with information about recognition and cleavage sites, isoschizomers, commercial availability, crystal and sequence data.
Roberts, Richard J. +3 more
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Restriction enzyme digestion of hemimethylated DNA [PDF]
Nucleic Acids Research, 1981 Hemimethylated duplex DNA of the bacteriophage phi X 174 was synthesized using primed repair synthesis is in vitro with E. coli DNA polymerase I followed by ligation to produce the covalently closed circular duplex (RFI). Single-stranded phi X DNA was used as a template, a synthetic oligonucleotide as primer and 5-methyldeoxycytidine-5'-triphosphate ...
Y, Gruenbaum, H, Cedar, A, Razin
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Use Cutting Enzymes to Encode the Secret Message [PDF]
Al-Rafidain Journal of Computer Sciences and Mathematics, 2014 Researchers begin to explore new methods to manage the huge amount of data shared over the Internet, in order to use them more efficiently and safely, One of the ways that is raised nowadays is Steganography, The art of information hiding within ...
Saadoun A.
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In vitro Type II Restriction of Bacteriophage DNA With Modified Pyrimidines
Frontiers in Microbiology, 2020 To counteract host-encoded restriction systems, bacteriophages (phages) incorporate modified bases in their genomes. For example, phages carry in their genomes modified pyrimidines such as 5-hydroxymethyl-cytosine (5hmC) in T4gt deficient in α- and β ...
Kiersten Flodman +4 more
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Revised selection criteria for candidate restriction enzymes in genome walking. [PDF]
PLoS ONE, 2012 A new method to improve the efficiency of flanking sequence identification by genome walking was developed based on an expanded, sequential list of criteria for selecting candidate enzymes, plus several other optimization steps.
Ali Taheri +3 more
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