Results 1 to 10 of about 392,148 (351)
Structures of the type I DNA restriction enzymes. [PDF]
Proc Natl Acad Sci U S A, 2017 The article by Liu et al. (1) on the structure of type I DNA restriction and modification enzymes purports to significantly advance our understanding of these enzymes and proposes a model for their operation. While the partial structure of one of these enzymes is interesting and defines the interface between some of the subunits, the article contains ...
Dryden DTF.
europepmc +5 more sources
REBASE--restriction enzymes and DNA methyltransferases [PDF]
Nucleic Acids Research, 2004 REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in restriction-modification. It contains both published and unpublished work with information about recognition and cleavage sites, isoschizomers, commercial availability, crystal and sequence data.
Richard J. Roberts
openalex +4 more sources
The average spacing of restriction enzyme recognition sites in DNA [PDF]
Journal of Theoretical Biology, 1982 The discovery of naturally occurring enzymes which cleave DNA at sites specific to particular nucleotide sequences has had a great impact on molecular biology. The function of these enzymes in uivo is to protect bacterial cells from viral invasion by degradation of foreign DNA.
Gordon P. Moore, A. R. Moore
openalex +5 more sources
Structure and operation of the DNA-translocating type I DNA restriction enzymes. [PDF]
Genes Dev, 2012 Type I DNA restriction/modification (RM) enzymes are molecular machines found in the majority of bacterial species. Their early discovery paved the way for the development of genetic engineering. They control (restrict) the influx of foreign DNA via horizontal gene transfer into the bacterium while maintaining sequence-specific methylation ...
Kennaway CK +15 more
europepmc +7 more sources
Site-specific DNA transesterification catalyzed by a restriction enzyme [PDF]
Proceedings of the National Academy of Sciences, 2007 Most restriction endonucleases use Mg 2+ to hydrolyze phosphodiester bonds at specific DNA sites. We show here that BfiI, a metal-independent restriction enzyme from the phospholipase D superfamily, catalyzes both DNA hydrolysis and transesterification reactions at its recognition site.
Giedrius Sasnauskas +3 more
openalex +6 more sources
REBASE—a database for DNA restriction and modification: enzymes, genes and genomes [PDF]
Nucleic Acids Research, 2009 Abstract REBASE is a comprehensive and extensively curated database of information about the components of restriction-modification (RM) systems. It is fully referenced and provides information about the recognition and cleavage sites for both restriction enzymes and DNA methyltransferases together with their commercial availability ...
Richard J. Roberts +3 more
openalex +6 more sources
Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering
Fermentation, 2023 Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
doaj +1 more source
Restriction enzyme digestion of hemimethylated DNA [PDF]
Nucleic Acids Research, 1981 Hemimethylated duplex DNA of the bacteriophage phi X 174 was synthesized using primed repair synthesis is in vitro with E. coli DNA polymerase I followed by ligation to produce the covalently closed circular duplex (RFI). Single-stranded phi X DNA was used as a template, a synthetic oligonucleotide as primer and 5-methyldeoxycytidine-5'-triphosphate ...
Howard Cedar +2 more
openaire +3 more sources
NEBcutter: a program to cleave DNA with restriction enzymes [PDF]
Nucleic Acids Research, 2003 NEBcutter, version 1.0, is a program available via a web server (http://tools.neb.com/NEBcutter) that will accept an input DNA sequence and produce a comprehensive report of the restriction enzymes that will cleave the sequence. It produces a variety of outputs including restriction enzyme maps, theoretical digests and links into the restriction enzyme
Richard J. Roberts +2 more
openaire +2 more sources
REBASE--enzymes and genes for DNA restriction and modification [PDF]
Nucleic Acids Research, 2007 REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in the biological process of restriction-modification. It contains fully referenced information about recognition and cleavage sites, isoschizomers, neoschizomers, commercial availability, methylation sensitivity, crystal ...
Richard J. Roberts +3 more
openaire +3 more sources