Results 1 to 10 of about 678,502 (268)

Structures of the type I DNA restriction enzymes. [PDF]

Proc Natl Acad Sci U S A, 2017
The article by Liu et al. (1) on the structure of type I DNA restriction and modification enzymes purports to significantly advance our understanding of these enzymes and proposes a model for their operation. While the partial structure of one of these enzymes is interesting and defines the interface between some of the subunits, the article contains ...
Dryden DTF.
europepmc   +5 more sources

REBASE--restriction enzymes and DNA methyltransferases [PDF]

Nucleic Acids Research, 2004
REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in restriction-modification. It contains both published and unpublished work with information about recognition and cleavage sites, isoschizomers, commercial availability, crystal and sequence data.
Roberts, Richard J., Vincze, Tamas, Posfai, Janos, Macelis, Dana   +3 more
openaire   +3 more sources

NEBcutter: a program to cleave DNA with restriction enzymes [PDF]

Nucleic Acids Research, 2003
NEBcutter, version 1.0, is a program available via a web server (http://tools.neb.com/NEBcutter) that will accept an input DNA sequence and produce a comprehensive report of the restriction enzymes that will cleave the sequence. It produces a variety of outputs including restriction enzyme maps, theoretical digests and links into the restriction enzyme
Tamas, Vincze, Janos, Posfai, Richard J, Roberts   +2 more
openaire   +3 more sources

Structure and operation of the DNA-translocating type I DNA restriction enzymes. [PDF]

Genes Dev, 2012
Type I DNA restriction/modification (RM) enzymes are molecular machines found in the majority of bacterial species. Their early discovery paved the way for the development of genetic engineering.
Kennaway CK, Taylor JEN, Song CF, Potrzebowski W, Nicholson W, White JH, Swiderska A, Obarska-Kosinska A, Callow P, Cooper LP, Roberts GA, Artero JB, Bujnicki JM, Trinick J, Kneale GG, Dryden DT.   +15 more
europepmc   +2 more sources

REBASE--enzymes and genes for DNA restriction and modification [PDF]

Nucleic Acids Research, 2007
REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in the biological process of restriction-modification. It contains fully referenced information about recognition and cleavage sites, isoschizomers, neoschizomers, commercial availability, methylation sensitivity, crystal ...
Roberts, Richard J., Vincze, Tamas, Posfai, Janos, Macelis, Dana   +3 more
openaire   +3 more sources

Site-specific DNA transesterification catalyzed by a restriction enzyme [PDF]

Proceedings of the National Academy of Sciences, 2007
Most restriction endonucleases use Mg 2+ to hydrolyze phosphodiester bonds at specific DNA sites. We show here that BfiI, a metal-independent restriction enzyme from the phospholipase D superfamily, catalyzes both DNA hydrolysis and transesterification reactions at its recognition site.
Giedrius Sasnauskas, Bernard A. Connolly, Stephen E. Halford, Virginijus Šikšnys   +3 more
openalex   +5 more sources

Physical map of bacteriophage BF23 DNA: restriction enzyme analysis [PDF]

Journal of Virology, 1979
Cleavage maps of bacteriophage BF23 DNA have been constructed for the restriction endonucleases SalI (3 fragments), BamHI (5 fragments), EcoRI, (8 fragments), BalI (13 fragments), and HpaI (49 fragments, 32 of which have been ordered). The maps were determined by (i) analysis of deletion mutants, (ii) digestion with two endonucleases, (iii) digestion ...
B Lange-Gustafson, M Rhoades
openalex   +3 more sources

The Cloning of the Human Tumor Supressor Gene INGI: DNA Cloning into Plasmid Vector and DNA Analysis by Restriction Enzymes

Journal of Dentistry Indonesia, 2015
DNA cloning is one of the most important techniques In the field of molecular biology, with a critical role in analyzing the structure and function of genes and their adjacent regulatory regions.
Elza Ibrahim Auerkari, Mamoru Ouchida, Mehmet Gunduz   +2 more
doaj   +2 more sources

Restriction enzymes use a 24 dimensional coding space to recognize 6 base long DNA sequences. [PDF]

PLoS ONE, 2019
Restriction enzymes recognize and bind to specific sequences on invading bacteriophage DNA. Like a key in a lock, these proteins require many contacts to specify the correct DNA sequence.
Thomas D Schneider, Vishnu Jejjala
doaj   +2 more sources

DNA Mapping Algorithms: Strategies for Single Restriction Enzyme and Multiple Restriction Enzyme Mapping

, 1992
An approach to high-resolution restriction-fragment DNA mapping, known as Multiple-Restriction-Enzyme mapping (MRE mapping), is present. This approach significantly reduces the uncertainty of clone placement by using clone ends to synchronize the position in of clones within different maps, each map being constructed from fragment-length data produced ...
Will Gillett
openalex   +4 more sources