Results 1 to 10 of about 225,319 (266)
Structures of the type I DNA restriction enzymes. [PDF]
Proc Natl Acad Sci U S A, 2017 The article by Liu et al. (1) on the structure of type I DNA restriction and modification enzymes purports to significantly advance our understanding of these enzymes and proposes a model for their operation. While the partial structure of one of these enzymes is interesting and defines the interface between some of the subunits, the article contains ...
Dryden DTF.
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Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering
Fermentation, 2023 Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
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REBASE--restriction enzymes and DNA methyltransferases [PDF]
Nucleic Acids Research, 2004 REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in restriction-modification. It contains both published and unpublished work with information about recognition and cleavage sites, isoschizomers, commercial availability, crystal and sequence data.
Roberts, Richard J. +3 more
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Restriction enzyme digestion of hemimethylated DNA [PDF]
Nucleic Acids Research, 1981 Hemimethylated duplex DNA of the bacteriophage phi X 174 was synthesized using primed repair synthesis is in vitro with E. coli DNA polymerase I followed by ligation to produce the covalently closed circular duplex (RFI). Single-stranded phi X DNA was used as a template, a synthetic oligonucleotide as primer and 5-methyldeoxycytidine-5'-triphosphate ...
Y, Gruenbaum, H, Cedar, A, Razin
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Use Cutting Enzymes to Encode the Secret Message [PDF]
Al-Rafidain Journal of Computer Sciences and Mathematics, 2014 Researchers begin to explore new methods to manage the huge amount of data shared over the Internet, in order to use them more efficiently and safely, One of the ways that is raised nowadays is Steganography, The art of information hiding within ...
Saadoun A.
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In vitro Type II Restriction of Bacteriophage DNA With Modified Pyrimidines
Frontiers in Microbiology, 2020 To counteract host-encoded restriction systems, bacteriophages (phages) incorporate modified bases in their genomes. For example, phages carry in their genomes modified pyrimidines such as 5-hydroxymethyl-cytosine (5hmC) in T4gt deficient in α- and β ...
Kiersten Flodman +4 more
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Revised selection criteria for candidate restriction enzymes in genome walking. [PDF]
PLoS ONE, 2012 A new method to improve the efficiency of flanking sequence identification by genome walking was developed based on an expanded, sequential list of criteria for selecting candidate enzymes, plus several other optimization steps.
Ali Taheri +3 more
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DNA polymorphism investigation among local Brucella isolates [PDF]
Iraqi Journal of Veterinary Sciences, 2009 The present study was undertaken to investigate the deoxyribonucleic acid (DNA) polymorphism finger printing among DNA of local Brucella isolates which is a new method in molecular epidemiological studies.
S. A. AL-Bayatti ¹ and A. N. Al-Thwani ²
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A physical map of human Alu repeats cleavage by restriction endonucleases
BMC Genomics, 2008 Background Alu repetitive elements are the abundant sequences in human genome. Diversity of DNA sequences of these elements makes difficult the construction of theoretical patterns of Alu repeats cleavage by restriction endonucleases.
Chernukhin Valery A +3 more
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BMC Evolutionary Biology, 2011 Background The discovery of restriction endonucleases and modification DNA methyltransferases, key instruments of genetic engineering, opened a new era of molecular biology through development of the recombinant DNA technology.
Solonin Alexander S +2 more
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