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A physical map of human Alu repeats cleavage by restriction endonucleases [PDF]

open access: yesBMC Genomics, 2008
Background Alu repetitive elements are the abundant sequences in human genome. Diversity of DNA sequences of these elements makes difficult the construction of theoretical patterns of Alu repeats cleavage by restriction endonucleases.
Chernukhin Valery A   +3 more
doaj   +5 more sources

Structures of the type I DNA restriction enzymes. [PDF]

open access: yesProc Natl Acad Sci U S A, 2017
The article by Liu et al. (1) on the structure of type I DNA restriction and modification enzymes purports to significantly advance our understanding of these enzymes and proposes a model for their operation. While the partial structure of one of these enzymes is interesting and defines the interface between some of the subunits, the article contains ...
Dryden DTF.
europepmc   +5 more sources

REBASE--restriction enzymes and DNA methyltransferases [PDF]

open access: greenNucleic Acids Research, 2004
REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in restriction-modification. It contains both published and unpublished work with information about recognition and cleavage sites, isoschizomers, commercial availability, crystal and sequence data.
Richard J. Roberts
openalex   +4 more sources

Fused eco29kIR- and M genes coding for a fully functional hybrid polypeptide as a model of molecular evolution of restriction-modification systems [PDF]

open access: yesBMC Evolutionary Biology, 2011
Background The discovery of restriction endonucleases and modification DNA methyltransferases, key instruments of genetic engineering, opened a new era of molecular biology through development of the recombinant DNA technology.
Solonin Alexander S   +2 more
doaj   +4 more sources

The average spacing of restriction enzyme recognition sites in DNA [PDF]

open access: greenJournal of Theoretical Biology, 1982
The discovery of naturally occurring enzymes which cleave DNA at sites specific to particular nucleotide sequences has had a great impact on molecular biology. The function of these enzymes in uivo is to protect bacterial cells from viral invasion by degradation of foreign DNA.
Gordon P. Moore, A. R. Moore
openalex   +5 more sources

On the DNA cleavage mechanism of Type I restriction enzymes [PDF]

open access: yesNucleic Acids Research, 2005
Although the DNA cleavage mechanism of Type I restriction–modification enzymes has been extensively studied, the mode of cleavage remains elusive. In this work, DNA ends produced by EcoKI, EcoAI and EcoR124I, members of the Type IA, IB and IC families ...
Bickle, Thomas A.   +4 more
core   +9 more sources

Structure and operation of the DNA-translocating type I DNA restriction enzymes. [PDF]

open access: yesGenes Dev, 2012
Type I DNA restriction/modification (RM) enzymes are molecular machines found in the majority of bacterial species. Their early discovery paved the way for the development of genetic engineering. They control (restrict) the influx of foreign DNA via horizontal gene transfer into the bacterium while maintaining sequence-specific methylation ...
Kennaway CK   +15 more
europepmc   +7 more sources

Site-specific DNA transesterification catalyzed by a restriction enzyme [PDF]

open access: greenProceedings of the National Academy of Sciences, 2007
Most restriction endonucleases use Mg 2+ to hydrolyze phosphodiester bonds at specific DNA sites. We show here that BfiI, a metal-independent restriction enzyme from the phospholipase D superfamily, catalyzes both DNA hydrolysis and transesterification reactions at its recognition site.
Giedrius Sasnauskas   +3 more
openalex   +6 more sources

REBASE—a database for DNA restriction and modification: enzymes, genes and genomes [PDF]

open access: goldNucleic Acids Research, 2009
Abstract REBASE is a comprehensive and extensively curated database of information about the components of restriction-modification (RM) systems. It is fully referenced and provides information about the recognition and cleavage sites for both restriction enzymes and DNA methyltransferases together with their commercial availability ...
Richard J. Roberts   +3 more
openalex   +6 more sources

Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering

open access: yesFermentation, 2023
Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
doaj   +1 more source

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