Results 1 to 10 of about 395,390 (239)
Endogenous Double-Stranded RNA [PDF]
Non-Coding RNA, 2021 The birth of long non-coding RNAs (lncRNAs) is closely associated with the presence and activation of repetitive elements in the genome. The transcription of endogenous retroviruses as well as long and short interspersed elements is not only essential for evolving lncRNAs but is also a significant source of double-stranded RNA (dsRNA).
Sadeq S +3 more
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Supercoiling Double-Stranded RNA [PDF]
Biophysical Journal, 2010 Through a novel "polymerase-stall" labeling procedure, we have successfully generated torsionally constrained molecules of double-stranded RNA (dsRNA). We have anchored these molecules within a magnetic tweezers apparatus, and by rotating the magnets, induced both positive and negative supercoils within these molecules.
Jan Lipfert +3 more
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RNA Interference by Single- and Double-stranded siRNA With a DNA Extension Containing a 3′ Nuclease-resistant Mini-hairpin Structure [PDF]
, 2009 Selective gene silencing by RNA interference (RNAi) involves double-stranded small interfering RNA (ds siRNA) composed of single-stranded (ss) guide and passenger RNAs.
Allison, Simon J., Milner, Jo
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Overstretching Double-Stranded RNA, Double-Stranded DNA, and RNA-DNA Duplexes [PDF]
Biophysical Journal, 2019 Using single-molecule force measurements, we compare the overstretching transition of the four types of duplexes composed of DNA or RNA strands. Three of the four extremities of each double helix are attached to two microscopic beads, and a stretching force is applied with a dual-beam optical trapping interferometer.
Melkonyan, Lena +3 more
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Neurodegeneration, Heterochromatin, and Double-Stranded RNA [PDF]
Journal of Experimental Neuroscience, 2019 Changes in chromatin and epigenetic modifications have been associated with aging and aging-associated neurodegenerative diseases, although the causal relationship between these changes and disease-related pathology has been unclear. Recent studies have now made direct connections between neurodegeneration-associated proteins and derepression of ...
Tassa K Saldi +3 more
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Characterization of Supercoiled Double-Stranded RNA, and Comparison to Double-Stranded DNA [PDF]
Biophysical Journal, 2011 While the mechanical properties of double-stranded DNA have been intensely studied using various single molecule assays, much less is known about double-stranded RNA (dsRNA). dsRNA is important in Biology as it forms the hereditary material for a number of viruses, and is also central to the RNA mediated gene-silencing pathway.
Skinner, Gary M. +4 more
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Widespread formation of double-stranded RNAs in testis [PDF]
Genome Research, 2021 The testis transcriptome is highly complex and includes RNAs that potentially hybridize to form double-stranded RNA (dsRNA). We isolated dsRNA using the monoclonal J2 antibody and deep-sequenced the enriched samples from testes of juvenileDicer1knockout mice, age-matched controls, and adult animals.
James E. Clark +10 more
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RNA interference in marine and freshwater sponges [PDF]
, 2011 Background: The marine sponge Tethya wilhelma and the freshwater sponge Ephydatia muelleri are emerging model organisms to study evolution, gene regulation, development, and physiology in non-bilaterian animal systems. Thus far, functional methods (i.e.,
Cieniewicz, Brandon +12 more
core +1 more source
MicroRNAs: Loquacious Speaks out [PDF]
, 2005 In Drosophila, Dicer-2 requires the double-stranded RNA binding protein R2D2, to mediate the assembly of short interfering RNAs into the RNA-induced silencing complex.
Leuschner, Philipp J.F. +2 more
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Silencing of double-stranded ribonuclease improves oral RNAi efficacy in southern green stinkbug Nezara viridula [PDF]
, 2021 Simple summary: RNAi interference (RNAi) is a conserved mechanism found in all eukaryotes. This mechanism is initiated by the presence of double-stranded RNA in the cells and leads to the blockage of protein synthesis of a target gene.
Christiaens, Olivier +3 more
core +1 more source