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Double-Stranded Viral RNA

1969
Publisher Summary The pace of research on RNA virus replication and the huge amount of related literature that has accumulated in the past 6 years attest to the wide interest in the topic. There has also been some controversy and apparent confusion, probably largely because of pressure for publication priority. In this chapter, methods for detecting,
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Gene silencing by double-stranded RNA

Current Opinion in Cell Biology, 2001
Eukaryotes silence gene expression in the presence of double-stranded RNA homologous to the silenced gene. Silencing occurs by the targeted degradation of mRNA. Biochemical reactions that recapitulate this phenomenon generate RNA fragments of 21--23 nucleotides from the double-stranded RNA.
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Assembly of Double‐Stranded RNA Bacteriophages

2005
Publisher Summary This chapter focuses on bacteriophage O6. Most of the current knowledge about the structure, assembly, and molecular biology of double stranded (ds) RNA bacteriophages is derived from model systems. It is found that the assembly of enveloped dsRNA bacteriophages can be dissected into four distinct steps: assembly of empty precursor ...
Roman Tuma   +2 more
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DOUBLE-STRANDED RNAS IN THE RUST FUNGI

Annual Review of Phytopathology, 1994
Rust fungi, of which there are some 5000 species in at least 100 genera, belong to the order Uredinales in the Basidiomycetes. Rusts are obligate parasites, with highly specialized relationships on a variety of host plant species ranging from ferns and gymnosperms to more highly evolved groups of the angio­ sperms such as legumes and grasses.
Zhang, Ren   +2 more
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Infectious double-stranded poliovirus RNA

Virology, 1964
Abstract Evidence is presented for the existence of an infectious double-stranded poliovirus RNA in HeLa cells which is relatively resistant to RNase digestion, has a buoyant density of 1.58 g cm −3 in Cs 2 SO 4 , a sedimentation coefficient of 16–20 S in sucrose gradients, and elutes from methylated albumin columns at 0.84 M NaCl.
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Direct Cloning of Double-Stranded RNAs

2015
Most annotated genomes show a large number of sense-antisense transcripts that can generate double-stranded RNAs. We describe a method to clone these dsRNAs from total RNA preparations.
Stefan Stamm   +3 more
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Interferon stimulated by Double Stranded RNA

Nature, 1970
Interferon represents an interesting approach to the control of viral disease in man and his domestic animals, as this review shows, for it is a part of the animal's own recovery processes during naturally occurring infection and active against most viruses that cause acute infection.
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RNA bulges and the helical periodicity of double-stranded RNA

Nature, 1990
RNA molecules typically exhibit extensive secondary structure, including double-stranded duplex, hairpins, internal loops, bulged bases and pseudoknotted structures (reviewed in refs 3 and 4). This is intimately connected with biological function, including splicing reactions and ribozyme activity.
Anamitra Bhattacharyya   +2 more
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RNA interference and double-stranded-RNA-activated pathways

Biochemical Society Transactions, 2004
RNAi (RNA interference) has become a powerful tool to determine gene function. Different methods of expressing the short ds (double-stranded) RNA intermediates required for interference in mammalian systems have been developed, including the introduction of si (short interfering) RNAs by direct transfection or driven from transfected plasmids or ...
Bryan R.G. Williams, Carol A Sledz
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Enigmatic double-stranded RNA in Japonica rice

Plant Molecular Biology, 1993
We have found a linear, 16 kb, double-stranded RNA (dsRNA) in symptomless Japonica rice (Oryza sativa L.) that is not found in Indica rice (Oryza sativa L.). The dsRNA was detected in every tissue and at every developmental stage, and its copy number was approximately constant (about 20 copies/cell). Double-stranded RNA was also detected in two strains
Hiroshi Hyakutake   +4 more
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