Results 31 to 40 of about 1,255,487 (165)

Potential of nanopore sequencing for tuberculosis diagnosis and drug resistance detection

BMC Infectious Diseases
Objectives This study evaluates the effectiveness of nanopore sequencing for accurate detection of Mycobacterium tuberculosis pathogens and drug resistance mutations in clinical specimens.
Fei Ren, JinBao Ma, LiYun Dang, AiFang Li, GuoLian Zhao, Yun Qi, You Xu, Han Yang, JianYing Li   +8 more
doaj   +1 more source

Identification of Mycobacterium tuberculosis intracellular survival-related virulence factors via CRISPR-based eukaryotic-like secretory protein mutant library screen

Microbiology Spectrum
Tuberculosis (TB), caused by Mycobacterium tuberculosis (M.tb), remains a serious infectious disease posing significant global health challenges. A critical evolutionary feature of M.tb is its genome encoding a set of eukaryotic-like secretory proteins ...
Weiyi Liu, Yingchao Wang, Jiayuan Zhao, Xinyue Zhang, Zihui Li, Hongyan Jia, Chuanzhi Zhu, Lanyue Zhang, Liping Pan, Zongde Zhang   +9 more
doaj   +1 more source

Novel and reported compensatory mutations in rpoABC genes found in drug resistant tuberculosis outbreaks

Frontiers in Microbiology
BackgroundRifampicin (RIF) is a key first-line drug used to treat tuberculosis, a primarily pulmonary disease caused by Mycobacterium tuberculosis. RIF resistance is caused by mutations in rpoB, at the cost of slower growth and reduced transcription ...
Derek Conkle-Gutierrez, Sarah M. Ramirez-Busby, Bria M. Gorman, Afif Elghraoui, Sven Hoffner, Sven Hoffner, Wael Elmaraachli, Faramarz Valafar   +7 more
doaj   +1 more source

Molecular detection of human Plasmodium species using a multiplex real time PCR

Scientific Reports, 2023
Molecular detection methods have revealed higher sensitivity and specificity than conventional microscopy or rapid diagnostic tests for malaria diagnosis.
Yassamine Lazrek, Célia Florimond, Béatrice Volney, Manon Discours, Emilie Mosnier, Sandrine Houzé, Stéphane Pelleau, Lise Musset   +7 more
doaj   +1 more source

Elucidation of critical chemical moieties of metallo-β-lactamase inhibitors and prioritisation of target metallo-β-lactamases

Journal of Enzyme Inhibition and Medicinal Chemistry
The urgent demand for effective countermeasures against metallo-β-lactamases (MBLs) necessitates development of novel metallo-β-lactamase inhibitors (MBLIs).
Jung Hun Lee, Sang-Gyu Kim, Kyung-Min Jang, Kyoungmin Shin, Hyeonku Jin, Dae-Wi Kim, Byeong Chul Jeong, Sang Hee Lee   +7 more
doaj   +1 more source

A approach of gastric conduit via the anterior of pulmonary hilum route during minimally invasive McKeown esophagectomy

Journal of Cardiothoracic Surgery
Background The gastric conduit is the most commonly used replacement organ for reconstruction after minimally invasive McKeown esophagectomy. Although the optimal route of gastric conduit remains controversial, the posterior mediastinal route is ...
Zhaoyang Yan, Xinjian Xu, Bin Guo, Pengzeng Wang, Linpeng Niu, Zhanjie Gao, Yusen Yuan, Fei Li, Ming He   +8 more
doaj   +1 more source

Circulating Epstein-Barr virus DNA and cell-free DNA in pediatric lymphomas

The Turkish Journal of Pediatrics, 2020
Background and Objectives. Quantification of serum Epstein-Barr virus (EBV) DNA and/or cell-free total DNA (cf-DNA) may become valuable sources for prognosis evaluation and monitoring treatment response in lymphomas.
Bilgehan Yalçın, Tezer Kutluk, Serpil Kahraman Ağbaba, Çetin Demir, Beril Talim   +4 more
doaj   +1 more source

Analysis of HIV-1 subtypes and drug resistance mutations in people who inject drugs in Aizawl and nearby districts of Mizoram, India

Virulence
Over the last decade, there has been a steady increase in HIV-1 prevalence in Mizoram, India. Importantly, this increase in HIV-1 prevalence is not only limited to the key population groups such as female sex workers (FSWs) or people who inject drugs ...
Komal Raskar, Lal Thlengliani, Andrew Lalbiaknunga, Dipali Kale, Ajit Patil, Pragati Chavan, Richard CLR Hluna, Samiran Panda, Swarali Kurle   +8 more
doaj   +1 more source

Efficacy of regimens targeting Mycobacterium abscessus in vitro and in vivo

BMC Microbiology
Background M. abscessus (Mabs) is one of the principal pathogenic strains among nontuberculous mycobacterial. Mabs infections pose a significant global public health challenge, leading to substantial morbidity and mortality. However, a standard treatment
Zimo Wang, Yangxue Ye, Weiyan Zhang, Bin Wang, Xiaoyou Chen, Yu Lu   +5 more
doaj   +1 more source

Updated Erdman reveals tandem repeat copy number is phase-variable and impacts M. tuberculosis adaptation across evolutionary timescales

mSystems
High-quality reference genomes are essential for comparative genomics and accurate genotype-phenotype mapping. Here, we corrected the Mycobacterium tuberculosis Erdman strain reference genome (ErdmanTI) using ultra-deep HiFi sequencing.
Samuel J. Modlin, Nachiket Thosar, Paulina M. Mejía-Ponce, Raegan L. Lunceford, Gaelle Guiewi Makafe, Brian Weinrick, Faramarz Valafar   +6 more
doaj   +1 more source