Results 21 to 30 of about 56,868 (220)
dsRNA Uptake in Adult Drosophila [PDF]
, 2011 RNA interference (RNAi) is a conserved sequence-specific gene silencing mechanism that is induced by double-stranded RNA (dsRNA). The development of methods that allow internalization of dsRNA and concomitant silencing of the desired gene has not stopped since the first demonstration of RNAi in Caenorhabditis elegans.
Obadia, Benjamin, Saleh, Maria-Carla
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Mechanism of PKR Activation by dsRNA [PDF]
Journal of Molecular Biology, 2008 Protein kinase R (PKR) is a central component of the interferon antiviral defense pathway. Upon binding double-stranded RNA (dsRNA), PKR undergoes autophosphorylation reactions that activate the kinase. PKR then phosphorylates eukaryotic initiation factor 2alpha, thus inhibiting protein synthesis in virally infected cells.
Peter A, Lemaire +3 more
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European Journal of Entomology, 2017 RNA interference (RNAi) technology uses dsRNAs to silence specific targeted genes by downregulating their expression. It has become a potent tool for functional and regulatory studies of insect genes and has potential to be applied for insect control ...
Saikat Kumar B. GHOSH +1 more
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RNA Biology, 2022 The RNase III family of dsRNA-specific endonucleases is exemplified by prokaryotic RNase III and eukaryotic Rnt1p, Drosha, and Dicer. Structures of Aquifex aeolicus RNase III (AaRNase III) and Saccharomyces cerevisiae Rnt1p (ScRnt1p) show that both ...
Sudhaker Dharavath +2 more
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Microinjection of dsRNA in Tardigrades [PDF]
Cold Spring Harbor Protocols, 2018 Classical genetic analysis in the tardigrade Hypsibius exemplaris is a challenge because these animals are parthenogens. The publication of the H. exemplaris genome has facilitated the study of targeted genes by RNA interference (RNAi), a robust mechanism to disrupt gene function. This protocol describes microinjection of double-stranded RNA (dsRNA) in
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RNAi-mediated gene silencing in Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae)
Open Life Sciences, 2017 RNA interference (RNAi) is a powerful strategy for gene function analysis, and it is also widely studied in view of a promising use in pest control. The red palm weevil, Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae), is one of the most ...
Laudani Francesca +7 more
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Biomolecules, 2020 Grapevine is one of the most important and globally widespread fruit species, with a high impact on the economy of many countries but with an intense environmental effect.
Luca Nerva +3 more
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An Investigation of Innate Immune Response of Human Blood Macrophage to Sense and Antisense dsRNA
Trends in Peptide and Protein Sciences, 2016 Silencing of gene expression by siRNA (small interfering RNA) is a powerful approach used to study the genetic analysis and functional roles of mammalian genes.
Nasrin Mohajeri +7 more
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Barriers to Efficient Foliar Uptake of dsRNA and Molecular Barriers to dsRNA Activity in Plant Cells [PDF]
Frontiers in Plant Science, 2020 Foliar application of dsRNA to elicit an RNA interference (RNAi) response is currently under consideration as a crop protection strategy. To access the RNAi machinery of a plant, foliarly applied dsRNAs must traverse the plant cuticle, avoid nuclease degradation, and penetrate the cell wall and plasma membrane.
Michael Bennett +3 more
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Quelling an innate response to dsRNA
Oncotarget, 2015 The interferon inducible oligoadenylate synthetase (OAS)/RNase L pathway is an innate defense against invading viral pathogens that is linked to cell survival [reviewed in 1]. OAS family proteins are pattern recognition receptors that recognize double-stranded RNA (dsRNA), a pathogen-associated molecular pattern.
Kristen M, Ogden, B V Venkataram, Prasad
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