Results 21 to 30 of about 73,913 (251)

Structural basis for Dicer-like function of an engineered RNase III variant and insights into the reaction trajectory of two-Mg2+-ion catalysis

RNA Biology, 2022
The RNase III family of dsRNA-specific endonucleases is exemplified by prokaryotic RNase III and eukaryotic Rnt1p, Drosha, and Dicer. Structures of Aquifex aeolicus RNase III (AaRNase III) and Saccharomyces cerevisiae Rnt1p (ScRnt1p) show that both ...
Sudhaker Dharavath, Gary X. Shaw, Xinhua Ji   +2 more
doaj   +1 more source

RNAi-mediated gene silencing in Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae)

Open Life Sciences, 2017
RNA interference (RNAi) is a powerful strategy for gene function analysis, and it is also widely studied in view of a promising use in pest control. The red palm weevil, Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae), is one of the most ...
Laudani Francesca, Strano Cinzia P., Edwards Martin G., Malacrinò Antonino, Campolo Orlando, Abd El Halim Hesham M., Gatehouse Angharad M. R., Palmeri Vincenzo   +7 more
doaj   +1 more source

Double-Stranded RNAs (dsRNAs) as a Sustainable Tool against Gray Mold (Botrytis cinerea) in Grapevine: Effectiveness of Different Application Methods in an Open-Air Environment

Biomolecules, 2020
Grapevine is one of the most important and globally widespread fruit species, with a high impact on the economy of many countries but with an intense environmental effect.
Luca Nerva, Marco Sandrini, Giorgio Gambino, Walter Chitarra   +3 more
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An Investigation of Innate Immune Response of Human Blood Macrophage to Sense and Antisense dsRNA

Trends in Peptide and Protein Sciences, 2016
Silencing of gene expression by siRNA (small interfering RNA) is a powerful approach used to study the genetic analysis and functional roles of mammalian genes.
Nasrin Mohajeri, Zarrin Sharifnia, Mojgan Bandehpour, Fatemeh Yarian, Amaneh Koochaki, Nariman Mosaffa, Kazem Parivar, Bahram Kazemi   +7 more
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(A,B) Northern gel blot analysis of CYP3-dsRNA and CYP3-dsRNA-derived siRNA accumulation in local and distal (semi-systemic) barley leaf areas.

, 2016
(A) Detection of 791 nt long CYP3-dsRNA precursor in pooled leaf tissue from non-infected leaves using [α-32P]-dCTP labeled CYP3-dsRNA as probe. Local (L) and distal (semi-systemic [S]) leaf segments were sampled separately at the indicated times after ...
John McMillan (3217584), Dagmar Biedenkopf (201484), Aline Koch (3217596), Vinitha Cardoza (3217605), Eltayb Abdellatef (3217602), Lennart Weber (479942), Alexandra Furch (3217590), Karl-Heinz Kogel (54116), Lukas Jelonek (839039), Alexander Goesmann (11354), Lukas Linicus (3217587), Tobias Mentzel (3217593), Jan Johannsmeier (3217581), Oliver Rossbach (3217599)   +13 more
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Design of Lipopeptides and dsRNA-Lipopeptide Complexes for Sprayable RNAi-Based Plant Protection. [PDF]

J Pept Sci
Lipopeptide surfactants self‐assemble into nanostructure carriers that complex, protect, and deliver dsRNA, establishing structure–function relationships for next‐generation RNAi‐based insect control. ABSTRACT Broad‐spectrum insecticides persist in the environment, affecting non‐target organisms and selecting for resistant pest populations. In contrast,
Geisler P, Steiniger F, Vilcinskas A, Hellmann C.   +3 more
europepmc   +2 more sources

(A,B) RNA profiling (RNAseq analysis) of CYP3-dsRNA-derived RNAs in local and distal tissue of CYP3-dsRNA-treated barley leaves on Illumina HiSeq.

, 2016
Higher numbers of reads of CYP3-dsRNA-derived sRNAs were found in infected vs. non-infected leaves both in the sprayed (local [A]) and non-sprayed (semi-systemic [B]) leaf area at 6 dpi with strain Fg-IFA65.
John McMillan (3217584), Dagmar Biedenkopf (201484), Aline Koch (3217596), Vinitha Cardoza (3217605), Eltayb Abdellatef (3217602), Lennart Weber (479942), Alexandra Furch (3217590), Karl-Heinz Kogel (54116), Lukas Jelonek (839039), Alexander Goesmann (11354), Lukas Linicus (3217587), Tobias Mentzel (3217593), Jan Johannsmeier (3217581), Oliver Rossbach (3217599)   +13 more
core   +1 more source

Physicochemical and toxicity investigation of chitosan-based dsRNA nanocarrier formation. [PDF]

, 2021
Thus, the present study is focused on the chitosan-dsRNA interactions and the physicochemical properties of the dsRNA-loaded nanoparticles, including cytotoxicity and hemolysis assays. dsRNA molecules homologous to a partial genome of the phytopathogenic
INOUE-NAGATA, A. K., Barros-Alexandrino, Tais T., Nagata, Alice K. I., LIMA, A. M. F., PILON, L., Pilon, Lucimeire, Petrônio, Maicon S. [UNESP], Tiera, Marcio J. [UNESP], NAKASU, E. Y. T., PETRÔNIO, M. S., TIERA, M. J., BARROS-ALEXANDRINO, T. T., ASSIS, O. B. G. de, Lima, Aline M. F. [UNESP], Nakasu, Erich Y. T., Assis, Odilio B. G., Lima, Aline M. F., Petrônio, Maicon S., ASSIS, O. B. G., Tiera, Marcio J.   +19 more
core   +1 more source

Spray-induced gene silencing in the battle against pathogens, pests and weeds on our fields - soon reality or still a sci-fi?

Plant Protection Science
Agriculture faces ongoing challenges from pathogens, pests and weed threats that are amplified by climate change, intensive monoculture farming practices, and regulatory restrictions limiting traditional chemical treatments.
Iveta Vachová, Jhonny Stalyn Hernández Orozco, Tereza Kalistová, Guy Smagghe, Martin Janda   +4 more
doaj   +1 more source

Formation of Double Stranded RNA Provokes Smooth Muscle Contractions and Structural Modifications in Bladder Ischemia

Research and Reports in Urology, 2022
Jing-Hua Yang,1 Zuohui Zhao,2 Wanting Niu,3 Han-Pil Choi,3 Kazem M Azadzoi4 1Department of Surgery, Boston University School of Medicine and Proteomics Laboratory, VA Boston Healthcare System, Boston, MA, USA; 2Department of Urology, Boston University ...
Yang JH, Zhao Z, Niu W, Choi HP, Azadzoi KM   +4 more
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