Results 41 to 50 of about 9,677 (245)

Characterization of the p22 Subunit of Dynactin Reveals the Localization of Cytoplasmic Dynein and Dynactin to the Midbody of Dividing Cells [PDF]

The Journal of Cell Biology, 1998
Dynactin, a multisubunit complex that binds to the microtubule motor cytoplasmic dynein, may provide a link between dynein and its cargo. Many subunits of dynactin have been characterized, elucidating the multifunctional nature of this complex. Using a dynein affinity column, p22, the smallest dynactin subunit, was isolated and microsequenced.
Bernadette H. LaMonte, Sher Bahadur Karki, Erika L.F. Holzbaur   +2 more
openaire   +3 more sources

Multivalency, autoinhibition, and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution protein

bioRxiv, 2022
Cytoplasmic dynein plays crucial roles in the intracellular transport of organelles and other cargoes. Central to dynein function is the intrinsically disordered N-terminal domain of dynein intermediate chain (IC), which binds the three dimeric dynein ...
Kayla A. Jara, N. Loening, P. Reardon, Zhenqin Yu, Prajna Woonnimani, Coban Brooks, C. Vesely, E. Barbar   +7 more
semanticscholar   +1 more source

The structure of the dynactin complex and its interaction with dynein [PDF]

Science, 2015
Making a molecular motor fit for purpose Dynactin is an essential cofactor of the microtubule motor, cytoplasmic dynein. Dynactin contains 23 subunits built around a short filament of an actin-related protein (Arp1). How dynactin is assembled, how it functions with dynein, and why it is built around an actin-like filament is ...
L. Urnavicius, Kai Zhang, A. G. Diamant, C. Motz, Max A. Schlager, Minmin Yu, Nisha A. Patel, C. Robinson, A. Carter   +8 more
semanticscholar   +4 more sources

Mutant dynactin in motor neuron disease [PDF]

Nature Genetics, 2003
Impaired axonal transport in motor neurons has been proposed as a mechanism for neuronal degeneration in motor neuron disease. Here we show linkage of a lower motor neuron disease to a region of 4 Mb at chromosome 2p13. Mutation analysis of a gene in this interval that encodes the largest subunit of the axonal transport protein dynactin showed a single
Robert H. Brown, Shin J. Oh, Kenneth H. Fischbeck, Erika L.F. Holzbaur, Imke Puls, Mary Kay Floeter, Kimberly Bidus, Bernadette H. LaMonte, Dennis Drayna, Catherine Jonnakuty, Mariko Tokito, Eric A. Mann, Christy L. Ludlow   +12 more
openaire   +3 more sources

Dynactin Is Required for Microtubule Anchoring at Centrosomes [PDF]

The Journal of Cell Biology, 1999
The multiprotein complex, dynactin, is an integral part of the cytoplasmic dynein motor and is required for dynein-based motility in vitro and in vivo. In living cells, perturbation of the dynein–dynactin interaction profoundly blocks mitotic spindle assembly, and inhibition or depletion of dynein or dynactin from meiotic or mitotic cell extracts ...
Steven R. Gill, Nicholas J. Quintyne, Duane A. Compton, C.L. Crego, Trina A. Schroer, D.M. Eckley   +5 more
openaire   +2 more sources

Conformational transitions of the mitotic adaptor Spindly underlie its interaction with Dynein and Dynactin

bioRxiv, 2022
Cytoplasmic Dynein 1, or Dynein, is a microtubule minus-end directed motor. Dynein motility requires Dynactin and a family of activating adaptors that stabilize the Dynein-Dynactin complex and promote regulated interactions with cargo in space and time ...
Ennio A d'Amico, Misbha Ud Din Ahmad, Verena Cmentowski, Mathias Girbig, Franziska Müller, Sabine Wohlgemuth, A. Brockmeyer, S. Maffini, P. Janning, I. Vetter, A. Carter, A. Perrakis, A. Musacchio   +12 more
semanticscholar   +1 more source

Interaction of tau protein with the dynactin complex [PDF]

The EMBO Journal, 2007
Tau is an axonal microtubule-associated protein involved in microtubule assembly and stabilization. Mutations in Tau cause frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17), and tau aggregates are present in Alzheimer's disease and other tauopathies.
Matthew Golding, Meredith Williams, Michel Goedert, Enrico Magnani, Juan Fan, Giampietro Schiavo, Linda A. Amos, Laura Gasparini, Maria Grazia Spillantini   +8 more
openaire   +3 more sources

LIS1, CLIP-170's Key to the Dynein/Dynactin Pathway [PDF]

Molecular and Cellular Biology, 2002
CLIP-170 is a plus-end tracking protein which may act as an anticatastrophe factor. It has been proposed to mediate the association of dynein/dynactin to microtubule (MT) plus ends, and it also binds to kinetochores in a dynein/dynactin-dependent fashion, both via its C-terminal domain. This domain contains two zinc finger motifs (proximal and distal),
Frédéric M. Coquelle, Michal Caspi, Fabrice P. Cordelières, Jim Dompierre, Denis Dujardin, Cynthia Koifman, Patrick Martin, Casper C. Hoogenraad, Anna Akhmanova, Niels Galjart, Jan R. De Mey, Orly Reiner   +11 more
openalex   +6 more sources

Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1

eLife, 2018
Cortical dynein generates pulling forces via microtubule (MT) end capture-shrinkage and lateral MT sliding mechanisms. In Saccharomyces cerevisiae, the dynein attachment molecule Num1 interacts with endoplasmic reticulum (ER) and mitochondria to ...
Safia Omer, Samuel R Greenberg, Wei-Lih Lee   +2 more
doaj   +1 more source

Dystonia and Optic Neuropathy: Expanded Phenotype of Dynactin 1 Related Neurodegeneration

Movement Disorders Clinical Practice, 2022
Axonal transport is essential to provide nutrients to axons and nerve terminals and to clear up misfolded proteins to avoid the accumulation of toxic aggregates.
S. Mehta, Abeer Goel, Deependra N. Singh, S. Ray, Basavaraj Tigari, A. Takkar, V. Lal   +6 more
semanticscholar   +1 more source