Results 21 to 30 of about 7,885 (177)
Dynamic recruitment of CDK5RAP2 to centrosomes requires its association with dynein. [PDF]
PLoS ONE, 2013 CDK5RAP2 is a centrosomal protein known to be involved in the regulation of the γ-tubulin ring complex and thus the organization of microtubule arrays. However, the mechanism by which CDK5RAP2 is itself recruited to centrosomes is poorly understood.
Yue Jia +4 more
doaj +1 more source
Dynactin Enhances the Processivity of Kinesin‐2 [PDF]
Traffic, 2006 Kinesin‐2 is a major microtubule‐based motor in most cell types. Its in vitro motile properties have been analyzed extensively and been found to differ considerably from kinesin‐1. Although recombinant kinesin‐2 heterodimers exhibit processive movement, the processivity of the native kinesin‐2 holoenzyme has never been evaluated. Kinesin‐2 can interact
Matthew A, Berezuk, Trina A, Schroer
openaire +2 more sources
Scientific Reports, 2018 The minus-end-directed microtubule motor cytoplasmic dynein requires the dynactin complex for in vivo functions. The backbone of the vertebrate dynactin complex is the Arp1 (actin-related protein 1) mini-filament whose barbed end binds to the ...
Jun Zhang, Rongde Qiu, Xin Xiang
doaj +1 more source
Regulation of mitochondria-dynactin interaction and mitochondrial retrograde transport in axons
eLife, 2017 Mitochondrial transport in axons is critical for neural circuit health and function. While several proteins have been found that modulate bidirectional mitochondrial motility, factors that regulate unidirectional mitochondrial transport have been harder ...
Catherine M Drerup +3 more
doaj +1 more source
Structural basis for cytoplasmic dynein-1 regulation by Lis1
eLife, 2022 The lissencephaly 1 gene, LIS1, is mutated in patients with the neurodevelopmental disease lissencephaly. The Lis1 protein is conserved from fungi to mammals and is a key regulator of cytoplasmic dynein-1, the major minus-end-directed microtubule motor ...
John P Gillies +6 more
doaj +1 more source
Dynactin Is Required for Microtubule Anchoring at Centrosomes [PDF]
The Journal of Cell Biology, 1999 The multiprotein complex, dynactin, is an integral part of the cytoplasmic dynein motor and is required for dynein-based motility in vitro and in vivo. In living cells, perturbation of the dynein–dynactin interaction profoundly blocks mitotic spindle assembly, and inhibition or depletion of dynein or dynactin from meiotic or mitotic cell extracts ...
N J, Quintyne +5 more
openaire +2 more sources
Mitochondrial adaptor TRAK2 activates and functionally links opposing kinesin and dynein motors
Nature Communications, 2021 Mitochondria are transported along microtubules by opposing kinesin and dynein motors. Kinesin-1 and dynein-dynactin are linked to mitochondria by TRAK proteins, but it is unclear how TRAKs coordinate these motors. We used single-molecule imaging of cell
Adam R. Fenton +3 more
doaj +1 more source
Motor-Assisted Co-Migration of Intracellular Organelles and Microtubules as a Mechanism for Directed Cargo Transport. [PDF]
BioessaysDirectional intracellular cargo transport is traditionally attributed to motor stepping along stationary microtubule (MT) tracks. We propose a complementary mechanism of cargo co‐migration with moving MTs. This mode requires cargo–MT tethering and directed MT motion.
Zhou C, Kengaku M.
europepmc +2 more sources
Spindly is required for rapid migration of human cells
Biology Open, 2018 Dynein is the sole processive minus-end-directed microtubule motor found in animals. It has roles in cell division, membrane trafficking, and cell migration.
Claudia Conte +3 more
doaj +1 more source
New insights into the mechanism of dynein motor regulation by lissencephaly-1
eLife, 2020 Lissencephaly (‘smooth brain’) is a severe brain disease associated with numerous symptoms, including cognitive impairment, and shortened lifespan. The main causative gene of this disease – lissencephaly-1 (LIS1) – has been a focus of intense scrutiny ...
Steven M Markus +2 more
doaj +1 more source