Results 51 to 60 of about 62,911 (266)

Purification of Human Elastase

Experimental Biology and Medicine, 1972
SummaryElastase was obtained from human pancreas by extraction with sodium acetate buffer, pH 4.5, fractionation with ammonium sulfate, and adsorption on powdered elastin. The enzyme was eluted from elastin with dilute acetic acid and precipitated with ammonium sulfate. An orcein–elastin substrate was used for assay of the enzymatic activity.
J O, Trowbridge, H D, Moon
openaire   +2 more sources

Microblasting Wound Dressings Mechanically Disrupt Polymicrobial Biofilms to Enhance Healing in Treatment‐Resistant Wounds

Advanced Science, EarlyView.
Treatment‐resistant wounds caused by polymicrobial biofilms are refractory to conventional therapies due to the dense extracellular matrices. We developed μBLAST, a microblasting wound dressing that combines MnO2‐doped biosilica and a H2O2‐releasing mesh to generate localized oxygen microbubbles that mechanically disrupt biofilms.
Yujin Ahn, Joo Hun Lee, Christian Hurd, Jiye Lee, Junggeon Park, Adam A. Markowicz, Zheyuan Zhang, Joanne Hwang, Guillermo L. Monroy, Simon A. Rogers, Woonggyu Jung, Stephen A. Boppart, Hyunjoon Kong   +12 more
wiley   +1 more source

p300 Degradation by the p53‐SIAH1 Axis Relieves TBK1 Acetylation to Enhance Innate Antiviral Immunity

Advanced Science, EarlyView.
This study identifies p300 as the acetyltransferase that acetylates TBK1 and inhibits its phosphorylation. Activation of the p53‐SIAH1 axis by immune response downregulates p300 expression to sustain innate antiviral immunity. Conditional p300 knockout in alveolar epithelial cells in vivo promotes antiviral responses and suppresses virus replication ...
Huidi Yu, Zhihao Zhan, Xiaoxiang Pan, Xinyue Zhang, Penggang Liu, Jing Sun, Xiulong Xu   +6 more
wiley   +1 more source

Correlation of PMN elastase and PMN elastase-to-neutrophil ratio with disease activity in patients with myositis

Journal of Translational Medicine, 2019
Background Polymorphonuclear (PMN) elastase plays an important role in a variety of inflammatory disorders. Our aim was to analyse PMN elastase in idiopathic inflammatory myopathies (IIMs) and its association with disease activity.
Siyu Wu, Wanchan Peng, Yunli Zhang, Jingjing Guo, Jinfang Fu, Wei Wang   +5 more
doaj   +1 more source

Evaluation of Elastase and α1-Proteinase Inhibitor-Elastase Uptake by Polymorphonuclear Leukocytes and Evidence of an Elastase-Specific Receptor [PDF]

Clinical Chemistry and Laboratory Medicine, 1986
Neither resting nor stimulated isolated human polymorphonuclear leukocytes did bind or ingest preformed complexes of alpha 1-proteinase inhibitor and unlabeled/125I-labeled human leukocyte elastase. In contrast, granulocytes bound unlabeled/125I-labeled elastase and the extent of binding was reduced in the presence of respiratory burst stimulators ...
Dwenger, A., Tost, P., Holle, W.
openaire   +2 more sources

A Blood‐Derived Double‐Network Hydrogel with Robust Wet Adhesion for Keratinized Mucosa Regeneration via Neutrophil Phenotype Reprogramming and Mechanophysical Niche Modulation

Advanced Science, EarlyView.
The iPRF‐MA/Alg‐NHS/Lut@EGCG (PGAE) double‐network hydrogel achieves durable wet adhesion in the oral environment. It simultaneously reprograms neutrophils from NETosis to phagocytosis for biofilm clearance, and activates the FAK‐RhoA‐YAP mechanotransduction pathway in gingival fibroblasts to drive extracellular matrix remodeling, thereby promoting ...
Sicong Ren, Xinting Yang, Jingxia Chen, Yihan Wang, Jian Feng, Jing Zhou, Jiaxin Luo, Jingjie Zhai, Quan Lin, Yanmin Zhou   +9 more
wiley   +1 more source

Detection of human elastase isoforms by the ScheBo Pancreatic Elastase 1 Test [PDF]

American Journal of Physiology-Gastrointestinal and Liver Physiology, 2017
Determination of fecal pancreatic elastase content by ELISA is a reliable, noninvasive clinical test for assessing exocrine pancreatic function. Despite the widespread use of commercial tests, their exact molecular targets remain poorly characterized.
Tóth Anna Zsófia, Szabó András, Hegyi Eszter, Hegyi Péter, Sahin-Tóth Miklós   +4 more
openaire   +4 more sources

Hyperacute Interleukin‐1β Production and Neutrophil Extracellular Trap Formation in the Cerebral Circulation of Stroke Patients with Large Vessel Occlusion

Annals of Neurology, EarlyView.
Acute ischemic stroke due to large vessel occlusion is associated with rapid intravascular immune activation. Analysis of arterial blood sampled distal to the thrombus during mechanical thrombectomy revealed increased extracellular adenosine triphosphate (ATP) and interleukin‐1β (IL‐1β) levels, evidence of inflammasome priming in monocytes, and ...
Justine Münsterberg, Caspar Brekenfeld, Hanna Englert, Marius Piepke, Regine J. Dress, Martin Oertel, Tina Fischer, Romy Hackbusch, Mingming Zha, Haodi Cai, Christian Casar, Ines S. Schädlich, Leo Winter, Alina Jander, Karoline Degenhardt, Maxim Bester, Fabian Flottmann, Uta Hanning, Brigitte Holst, Anna Worthman, Johanna Hiefner, Berenike Rutz, Eckhard Schlemm, Götz Thomalla, Bettina H. Clausen, Ann M. Stowe, Immo Prinz, Thiruma V. Arumugam, Markus Glatzel, Thomas Renné, Jens Fiehler, Eva Tolosa, Tim Magnus, Mathias Gelderblom   +33 more
wiley   +1 more source

Previous COVID-19 infection significantly reduces elastase levels in newly diagnosed pulmonary tuberculosis patients

Frontiers in Immunology
IntroductionTuberculosis (TB) is considered a risk factor for severe COVID-19 disease and the quality of life of patients co-infected with COVID-19 and TB is significantly impacted due to the nature of these diseases.
Nancy Hilda Joseph, Alangudi Palaniappan Natarajan, Saravanan Natarajan, Chinnaiyan Ponnuraja, Madeshwaran A, Gunaparvathy I, Hemalatha P, Rajeshwari S, Lavanya Jayabal, Mahilmaran Ayyamperumal, Ramesh P. M., Luke Elizabeth Hanna   +11 more
doaj   +1 more source

The role of secretory leukocyte proteinase inhibitor and elafin (elastase-specific inhibitor/skin-derived antileukoprotease) as alarm antiproteinases in inflammatory lung disease

Respiratory Research, 2000
Secretory leukocyte proteinase inhibitor and elafin are two low-molecular-mass elastase inhibitors that are mainly synthesized locally at mucosal sites. It is thought that their physicochemical properties allow them to efficiently inhibit target enzymes,
Sallenave Jean-Michel
doaj   +1 more source