Results 121 to 130 of about 5,598 (174)
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In vivo plasmid DNA electrotransfer
Current Opinion in Biotechnology, 2002In vivo electrotransfer is a physical technique for gene delivery in various mammalian tissues, which involves the injection of plasmid DNA into a target tissue and administration of an electric field. Its ease of performance, as well as recent understanding of its mechanism and applications to different mammalian tissues such as skeletal muscle, liver,
Daniel Scherman, Pascal Bigey
exaly +3 more sources
Optimisation of intradermal DNA electrotransfer for immunisation
Journal of Controlled Release, 2007The development of DNA vaccines requires appropriate delivery technologies. Electrotransfer is one of the most efficient methods of non-viral gene transfer. In the present study, intradermal DNA electrotransfer was first optimised. Strong effects of the injection method and the dose of DNA on luciferase expression were demonstrated. Pre-treatments were
Gaëlle Vandermeulen, Daniel Scherman
exaly +3 more sources
Electrotransfer for nucleic acid and protein delivery
Trends in BiotechnologyElectrotransfer of nucleic acids and proteins has become crucial in biotechnology for gene augmentation and genome editing. This review explores the applications of electrotransfer in both ex vivo and in vivo scenarios, emphasizing biomedical uses. We provide insights into completed clinical trials and successful instances of nucleic acid and protein ...
Pouyan Boukany, Aswin Muralidharan
exaly +3 more sources
The Role of Electrophoresis in Gene Electrotransfer
The Journal of Membrane Biology, 2010Gene electrotransfer is an established method for gene delivery which uses high-voltage pulses to increase the permeability of a cell membrane and enables transfer of genes. Poor plasmid mobility in tissues is one of the major barriers for the successful use of gene electrotransfer in gene therapy.
M, Pavlin, K, Flisar, M, Kanduser
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Imaging of Electrotransferred siRNA
2016SiRNA delivery to the cytoplasm can be obtained through the application of calibrated electric field pulses to a mixture of cells and oligonucleotides. To investigate the uptake pathway, time lapse confocal fluorescence microscopy provides a direct visualization of the transfer.
Muriel, Golzio, Justin, Teissié
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Efficient DNA electrotransfer into tumors
Bioelectrochemistry, 2000DNA transfer to tumor cells of antiproliferative genes or of genes coding for immunomodulatory or antiangiogenic products is a promising approach for cancer therapy. However, intratumoral injection of plasmid DNA either naked or associated to chemical vectors results in a low level of gene expression.
M, Bettan +5 more
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DNA and Cell Biology, 2002
The use of electrotransfer for DNA delivery to prokaryotic cells, and eukaryotic cells in vitro, has been well known and widely used for many years. However, it is only recently that electric fields have been used to enhance DNA transfer to animal cells in vivo, and this is known as DNA electrotransfer or in vivo DNA electroporation.
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The use of electrotransfer for DNA delivery to prokaryotic cells, and eukaryotic cells in vitro, has been well known and widely used for many years. However, it is only recently that electric fields have been used to enhance DNA transfer to animal cells in vivo, and this is known as DNA electrotransfer or in vivo DNA electroporation.
openaire +5 more sources
Gene Electrotransfer Clinical Trials
2015Plasmid or non-viral gene therapy offers an alternative to classic viral gene delivery that negates the need for a biological vector. In this case, delivery is enhanced by a variety of approaches including lipid or polymer conjugation, particle-mediated delivery, hydrodynamic delivery, ultrasound or electroporation.
Richard, Heller, Loree C, Heller
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Electrotransfer into Skeletal Muscle for Protein Expression
Current Gene Therapy, 2006An efficient and safe method to deliver DNA in vivo is a requirement for several purposes, such as study of gene function and gene therapy applications. Among the different non-viral delivery methods currently under investigation, in vivo DNA electrotransfer has proven to be one of the most efficient and simple.
C, Trollet +3 more
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