Results 141 to 150 of about 5,598 (174)
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Cationic electrophoresis and electrotransfer of membrane glycoproteins
Analytical Biochemistry, 2003Sodium dodecyl sulfate electrophoresis of glycoproteins results in broad, fuzzy bands. The use of the positively charged detergent cetyltrimethylammonium bromide (CTAB) avoids this problem. Protocols for CTAB electrophoresis, electroblotting, and protein detection are presented. These methods were found useful in studies on the multiple drug resistance
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Calcium influx determines the muscular response to electrotransfer
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2012Cell membrane permeabilization by electric pulses (electropermeabilization), results in free exchange of ions across the cell membrane. The role of electrotransfer-mediated Ca2+-influx on muscle signaling pathways involved in degeneration (β-actin and MurF), inflammation (IL-6 and TNF-α), and regeneration (MyoD1, myogenin, and Myf5) was investigated ...
Møller, Pernille Højman +2 more
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In vivo gene expression: DNA electrotransfer.
Current opinion in molecular therapeutics, 2003The use of electric pulses to deliver therapeutic molecules to tissues and organs in vivo is a rapidly growing field of research. Electrotransfer can be used to deliver a wide range of potentially therapeutic agents, including drugs, proteins, oligonucleotides, RNA and DNA.
Trezise, A. E. +3 more
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Electrotransfer of RNAi-based oligonucleotides for oncology.
Anticancer research, 2012For more than a decade, there has been tremendous growth in our understanding of RNA interference (RNAi). The potent ability that small oligonucleotides have in gene silencing makes them desirable as novel cancer therapeutics, but many biological barriers exist for their efficient delivery into target cells or tissues.
Pelofy, Sandrine +4 more
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RNA Blotting by Electrotransfer and RNA Detection
Northern blot, or RNA blot, is a widely used technique in molecular biology to detect and semi-quantify specific RNAs. The advantage of this method is its ability to simultaneously estimate the sizes and quantities of degraded or processed RNA products. Northern blotting involves the use of electrophoresis to separate RNA samples by size.Gabin, Ferrand +4 more
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Basic Principles and Clinical Advancements of Muscle Electrotransfer
Current Gene Therapy, 2010Muscle electrotransfer covers the delivery of molecules to muscle tissue by means of electric pulses. This method has proven highly efficient in transferring, in particular, plasmid DNA to muscles, resulting in long-term expression of the transferred genes.
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Electrotransfer of therapeutic molecules into tissues.
Current opinion in molecular therapeutics, 2008Electroporation is a physical method for the delivery of various molecules into cells by application of controlled external electrical fields that transiently increase permeability of the cell membrane. This technique is now widely used as an alternative to viral gene delivery for transfection of therapeutic genes into different tissues.
Maja, Cemazar, Gregor, Sersa
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