Results 131 to 140 of about 5,598 (174)
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2011
In vivo electroporation is a versatile delivery method for gene transfer which can be applied to any accessible tissue. Delivery of plasmid DNA encoding therapeutic genes or cDNAs with in vivo electroporation has been tested extensively in preclinical cancer models.
Loree C. Heller, Richard Heller
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In vivo electroporation is a versatile delivery method for gene transfer which can be applied to any accessible tissue. Delivery of plasmid DNA encoding therapeutic genes or cDNAs with in vivo electroporation has been tested extensively in preclinical cancer models.
Loree C. Heller, Richard Heller
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2011
Gene electrotransfer to skin is achieving increasing interest and is likely to gain considerable clinical application due to the ease with which it is performed and the safety of the procedure. There is a potential use of gene electrotransfer to skin in e.g., DNA vaccinations, local production of therapeutic molecules as well as production of molecules
Anita Gothelf, Julie Gehl
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Gene electrotransfer to skin is achieving increasing interest and is likely to gain considerable clinical application due to the ease with which it is performed and the safety of the procedure. There is a potential use of gene electrotransfer to skin in e.g., DNA vaccinations, local production of therapeutic molecules as well as production of molecules
Anita Gothelf, Julie Gehl
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Electrotransfer to Nylon Membranes
1987One of the most important and technically difficult aspects of genomic sequencing is the electrophoretic transfer of the genomic DNA from the sequencing gel to the nylon membrane. Many different parameters have to be taken into consideration: electrical resistance, strength of the electric field, diffusion, Joule heating, stability of the buffer, etc ...
Hanspeter Saluz, Jean-Pierre Jost
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Electrotransfer of Plasmid DNA
2011Various vectors have been developed in the field of gene transfer. However, important unresolved problems persist such as secondary toxicity or low gene transfer efficiency of viral as well as nonviral vectors. Therefore, an efficient and safe method of DNA delivery needs to be found.
Jean-Michel Escoffre +2 more
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Gene Electrotransfer in Clinical Trials
2014Electroporation is increasingly being used for delivery of chemotherapy to tumors. Likewise, gene delivery by electroporation is rapidly gaining momentum for both vaccination purposes and for delivery of genes coding for other therapeutic molecules, such as chronic diseases or cancer.
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Biomolecule electrotransfer to mammalian cells
2022Delivery of biomolecules using pulsed electric fields or electrotransfer has applications such as biomedical engineering, bioprocess engineering and genomic engineering. When a cell is placed in an electric field, the induced transmembrane voltage catalyzes the formation of pores on the cell membrane.
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Electrotransfer as a Non Viral Method of Gene Delivery
Current Gene Therapy, 2007Over the last few decades, various vectors have been developed in the field of gene therapy. There still exist a number of important unresolved problems associated with the use of viral as well as non viral vectors. These techniques can suffer from secondary toxicity or low gene transfer efficiency.
Favard, Cyril +2 more
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Drug and Gene Electrotransfer to the Brain
2011Applying drugs and genes to brain tissue using electroporation is a new and upcoming field of research. Because of the blood brain barrier (BBB), the access of agents from the blood into brain tissue is restricted. This could theoretically be overcome by the use of electroporation.
Birgit Agerholm-Larsen +3 more
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2011
Electroporation has proven to be a highly effective technique for the in vivo delivery of genes to a number of solid tissues. In most of the reported methods, DNA is injected into the target tissue and electrodes are placed directly on or in the tissue for application of the electric field.
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Electroporation has proven to be a highly effective technique for the in vivo delivery of genes to a number of solid tissues. In most of the reported methods, DNA is injected into the target tissue and electrodes are placed directly on or in the tissue for application of the electric field.
openaire +1 more source
Genetic Immunization with Plasmid DNA Mediated by Electrotransfer
Human Gene Therapy, 2011The concept of DNA immunization was first advanced in the early 1990s, but was not developed because of an initial lack of efficiency. Recent technical advances in plasmid design and gene delivery techniques have allowed renewed interest in the idea.
Alice, Rochard +2 more
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