163. Hepatocyte Targeted Endosomal Escape Agents [PDF]
Molecular Therapy, 2015 The successful development of potent non-viral DNA and mRNA delivery agents to transfect liver hepatocytes in vivo requires the use of an endosomal escape molecule. Two major strategies have emerged, one that utilizes membrane lytic peptides, and a second that uses proton sponge polymers. Both approaches would require administration of excess endosomal
White, Christopher W. +2 more
openaire +1 more source
Cytosolic delivery of nucleic acids: The case of ionizable lipid nanoparticles
Bioengineering & Translational Medicine, 2021 Ionizable lipid nanoparticles (LNPs) are the most clinically advanced nano‐delivery system for therapeutic nucleic acids. The great effort put in the development of ionizable lipids with increased in vivo potency brought LNPs from the laboratory benches ...
Michele Schlich +6 more
doaj +1 more source
Selective targeting of chemically modified miR-34a to prostate cancer using a small molecule ligand and an endosomal escape agent. [PDF]
Mol Ther Nucleic AcidsUse of tumor-suppressive microRNAs (miRNAs) as anti-cancer agents is hindered by the lack of effective delivery vehicles, entrapment of the miRNA within endocytic compartments, and rapid degradation of miRNA by nucleases.
Abdelaal AM +8 more
europepmc +2 more sources
Poly(2-propylacrylic acid)/poly(lactic-co-glycolic acid) blend microparticles as a targeted antigen delivery system to direct either CD4+ or CD8+ T cell activation. [PDF]
, 2017 Poly(lactic-co-glycolic acid) (PLGA) based microparticles (MPs) are widely investigated for their ability to load a range of molecules with high efficiency, including antigenic proteins, and release them in a controlled manner.
Bracho-Sanchez, Evelyn +6 more
core +1 more source
Nanomaterials, 2021 The ability to predict the behaviour of polymeric nanomedicines can often be obfuscated by subtle modifications to the corona structure, such as incorporation of fluorophores or other entities.
Joshua D. Simpson +7 more
doaj +1 more source
Thiol-Disulfide Exchange as a Route for Endosomal Escape of Polymeric Nanoparticles. [PDF]
Angew Chem Int Ed Engl, 2022 Endosomal entrapment has remained the major bottleneck for cytosolic delivery of nanoparticle-based delivery systems. Uncovering fundamentally new pathways for endosomal escape is therefore highly sought.
Kanjilal P, Dutta K, Thayumanavan S.
europepmc +2 more sources
A display of pH-sensitive fusogenic GALA peptide facilitates endosomal escape from a Bio-nanocapsule via an endocytic uptake pathway [PDF]
, 2014 BACKGROUND: An affibody-displaying bio-nanocapsule (Z(HER2)-BNC) with a hepatocyte specificity derived from hepatitis B virus (HBV) was converted into an affibody, Z(HER2), that recognizes HER2 receptors.
Akihiko Kondo +5 more
core +1 more source
Acid-Labile Traceless Click Linker for Protein Transduction [PDF]
, 2012 Intracellular delivery of active proteins presents an interesting approach in research and therapy. We created a protein transduction shuttle based on a new traceless click linker that combines the advantages of click reactions with implementation of ...
Bachelder E. M. +21 more
core +1 more source
Acid-labile chemical bonds-based nanoparticles for endosome escape and intracellular delivery
Biomedical Technology, 2023 In the last years there has been a booming in the development of biological pharmaceuticals, and various nanoparticle delivery systems have been developed to overcome biological barriers to maximize the therapeutic potentials. Especially, when these drug-
Ruoyu Cheng +2 more
doaj +1 more source
Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins. [PDF]
, 2018 New methods for delivering proteins into the cytosol of mammalian cells are being reported at a rapid pace. Differentiating between these methods in a quantitative manner is difficult, however, as most assays for evaluating cytosolic protein delivery are
Knox, Susan L +4 more
core +2 more sources