Results 11 to 20 of about 88,833 (257)

Streamlined scanning for enhancer elements in Drosophila melanogaster

BioTechniques, 2016
Enhancer elements in most eukaryotic organisms are often positioned at a great distance away from the transcription start site of the gene they regulate. Complex three-dimensional chromatin organization and insulators usually guide and limit the range of
Roumen Voutev, Richard S. Mann
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Technical and biological sources of noise confound multiplexed enhancer AAV screening [PDF]

Nature Communications
Cis-acting regulatory enhancer elements are powerful tools for achieving cell type-specific genetic access in adeno-associated virus (AAV) delivery platforms.
Avery C. Hunker, John K. Mich, Naz Taskin, Amy Torkelson, Trangthanh Cardenas, Jean-Benoît Lalanne, Joseph T. Mahoney, Darren Bertagnolli, Anish Bhaswanth Chakka, Rushil Chakrabarty, Nicholas P. Donadio, Rebecca Ferrer, Molly Gasperini, Jeff Goldy, Junitta Guzman, Kelly Jin, Shannon Khem, Rana Kutsal, Refugio A. Martinez, Dakota Newman, Nick Pena, Christine Rimorin, Dana Rocha, Nadiya V. Shapovalova, Michael Tieu, Natalie Weed, Thomas Zhou, Rebecca Hodge, Shenqin Yao, Jay Shendure, Kimberly A. Smith, Ed S. Lein, Bosiljka Tasic, Boaz P. Levi, Jonathan T. Ting   +34 more
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Functional interrogation of candidate cis-regulatory elements at the LDLR locus. [PDF]

PLoS Genetics
Regulation of LDLR gene expression plays an important role in the development of atherosclerotic diseases including heart attack and stroke. Although LDLR regulation by sterol response elements has been well characterized, the functional significance of ...
Kyle Leix, Candilianne Serrano-Zayas, Hitarthi S Vyas, Sarah E Graham, Brian T Emmer   +4 more
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RWDisEnh+: Enhancing disease-enhancer association prediction through multiplex-heterogeneous networks. [PDF]

PLoS ONE
Enhancers are critical regulatory DNA elements that, when dysregulated, can contribute to disease pathogenesis by altering gene expression. Although millions of enhancers have been identified through large-scale genomic projects, their associations with ...
Duc-Hau Le
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Enhancer reprogramming in mammalian genomes

BMC Bioinformatics, 2018
Background Transcription factor binding site (TFBS) loss, gain, and reshuffling within the sequence of a regulatory element could alter the function of that regulatory element.
Mario A. Flores, Ivan Ovcharenko
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Haplotype-specific chromatin looping reveals genetic interactions of regulatory regions modulating gene expression in 8p23.1

Frontiers in Genetics, 2022
A major goal of genetics research is to elucidate mechanisms explaining how genetic variation contributes to phenotypic variation. The genetic variants identified in genome-wide association studies (GWASs) generally explain only a small proportion of ...
Mariana Saint Just Ribeiro, Pulak Tripathi, Bahram Namjou, John B. Harley, John B. Harley, Iouri Chepelev, Iouri Chepelev   +6 more
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Parallel functional testing identifies enhancers active in early postnatal mouse brain

eLife, 2021
Enhancers are cis-regulatory elements that play critical regulatory roles in modulating developmental transcription programs and driving cell-type-specific and context-dependent gene expression in the brain. The development of massively parallel reporter
Jason T Lambert, Linda Su-Feher, Karol Cichewicz, Tracy L Warren, Iva Zdilar, Yurong Wang, Kenneth J Lim, Jessica L Haigh, Sarah J Morse, Cesar P Canales, Tyler W Stradleigh, Erika Castillo Palacios, Viktoria Haghani, Spencer D Moss, Hannah Parolini, Diana Quintero, Diwash Shrestha, Daniel Vogt, Leah C Byrne, Alex S Nord   +19 more
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Genetic variations within human gained enhancer elements affect human brain sulcal morphology

NeuroImage, 2023
The expansion of the cerebral cortex is one of the most distinctive changes in the evolution of the human brain. Cortical expansion and related increases in cortical folding may have contributed to emergence of our capacities for high-order cognitive ...
Herve Lemaitre, Yann Le Guen, Amanda K. Tilot, Jason L. Stein, Cathy Philippe, Jean-François Mangin, Simon E. Fisher, Vincent Frouin   +7 more
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Characterization of transcriptional enhancers in the chicken genome using CRISPR-mediated activation

Frontiers in Genome Editing, 2023
DNA regulatory elements intricately control when, where, and how genes are activated. Therefore, understanding the function of these elements could unveil the complexity of the genetic regulation network.
Jeong Hoon Han, Hong Jo Lee, Tae Hyun Kim, Tae Hyun Kim   +3 more
doaj   +1 more source

Global chromatin accessibility profiling analysis reveals a chronic activation state in aged muscle stem cells

iScience, 2022
Summary: Regulation of chromatin accessibility is critical for cell fate decisions. Chromatin structure responds to extrinsic environments rapidly. The traditional adult stem cell isolation approach requires tissue dissociation, which triggers stem cell ...
Anqi Dong, Jing Liu, Kangning Lin, Wenshu Zeng, Wai-Kin So, Shenyuan Hu, Tom H. Cheung   +6 more
doaj   +1 more source