Generation of equine enteroids and enteroid-derived 2D monolayers that are responsive to microbial mimics [PDF]
Veterinary Research, 2021 Enteroid cultures are three-dimensional in vitro models that reflect the cellular composition and architecture of the small intestine. One limitation with the enteroid conformation is the enclosed lumen, making it difficult to expose the apical surface ...
Stina Hellman
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Establishment of bovine 3D enteroid-derived 2D monolayers [PDF]
Veterinary Research, 2022 Three-dimensional (3D) intestinal enteroids are powerful in vitro models for studying intestinal biology. However, due to their closed structure direct access to the apical surface is impeded, limiting high-throughput applications of exogenous compounds ...
Kate M. Sutton +4 more
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Organoid and Enteroid Modeling of Salmonella Infection [PDF]
Frontiers in Cellular and Infection Microbiology, 2018 Salmonella are Gram-negative rod-shaped facultative anaerobic bacteria that are comprised of over 2,000 serovars. They cause gastroenteritis (salmonellosis) with headache, abdominal pain and diarrhea clinical symptoms. Salmonellosis brings a heavy burden
Yuebang Yin, Daoguo Zhou, Daoguo Zhou
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Equine enteroid-derived monolayers recapitulate key features of parasitic intestinal nematode infection [PDF]
Veterinary ResearchStem cell-derived organoid cultures have emerged as attractive experimental models for infection biology research regarding various types of gastro-intestinal pathogens and host species.
Stina Hellman +7 more
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Temporal transcriptome profiling of floating apical out chicken enteroids suggest stability and reproducibility [PDF]
Veterinary Research, 2023 Enteroids are miniature self-organising three-dimensional (3D) tissue cultures which replicate much of the complexity of the intestinal epithelium.
Tessa J. Nash +3 more
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Human Small Intestinal Tissue Models to Assess Barrier Permeability: Comparative Analysis of Caco-2 Cells, Jejunal and Duodenal Enteroid-Derived Cells, and EpiIntestinalTM Tissues in Membrane-Based Cultures with and Without Flow [PDF]
BioengineeringAccurate in vitro models of intestinal permeability are essential for predicting oral drug absorption. Standard models like Caco-2 cells have well-known limitations, including lack of segment-specific physiology, but are widely used. Emerging models such
Haley L. Moyer +9 more
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IFN-γ-Induced intestinal epithelial cell-type-specific programmed cell death: PANoptosis and its modulation in Crohn’s disease [PDF]
Frontiers in ImmunologyBackgroundCrohn’s disease (CD) is a chronic inflammatory bowel disease (IBD) and is considered a Th1-mediated disease, supported by the over-expression of interferon-gamma (IFN-γ) in the intestinal lamina propria.
Chansu Lee +10 more
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Fibroblasts enhance the growth and survival of adult feline small intestinal organoids [PDF]
mSphereIntestinal organoids are important cell culture models that complement live animal studies of many intestinal pathogens. Adult feline small intestinal organoids are needed for infectious disease research but are difficult to work with due to slow growth ...
Nicole D. Hryckowian +5 more
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An Open-Format Enteroid Culture System for Interrogation of Interactions Between Toxoplasma gondii and the Intestinal Epithelium [PDF]
Frontiers in Cellular and Infection Microbiology, 2019 When transmitted through the oral route, Toxoplasma gondii first interacts with its host at the small intestinal epithelium. This interaction is crucial to controlling initial invasion and replication, as well as shaping the quality of the systemic ...
Lisa Luu +9 more
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Apical-out bovine intestinal organoids as an infection model for Cryptosporidium parvum [PDF]
Current Research in Parasitology and Vector-Borne DiseasesCryptosporidium parvum is a major pathogen responsible for neonatal calf diarrhoea, but research has been hampered due to the lack of in vitro models that can complete the life cycle.
Mathilde Svensen Varegg +4 more
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