Results 11 to 20 of about 2,925 (189)

Whole-mount Enteroid Proliferation Staining [PDF]

Bio-Protocol, 2016
Small intestinal organoids, otherwise known as enteroids, have become an increasingly utilized model for intestinal biology in vitro as they recapitulate the various epithelial cells within the intestinal crypt (Mahe et al., 2013; Sato et al., 2009 ...
Caitlyn Barrett, Sarah Short, Yash Choksi , Christopher Williams   +3 more
doaj   +3 more sources

Human enteroids: Preclinical models of non-inflammatory diarrhea [PDF]

Stem Cell Research & Therapy, 2013
Researchers need an available and easy-to-use model of the human intestine to better understand human intestinal physiology and pathophysiology of diseases, and to offer an enhanced platform for developing drug therapy.
Broughman, J.R. (James), Donowitz, M. (Mark), Estes, M. (Mary), Ettayebi, K. (Khalil), Foulke-Abel, J. (Jennifer), Hyser, J.M. (Joseph), In, J. (Julie), Jonge, H.R. (Hugo) de, Kovbasnjuk, O. (Olga), Middendorp, S., Zachos, N.C. (Nicholas C.), Zeng, X.-L. (Xi-Lei)   +11 more
core   +4 more sources

Intestinal Enteroid Culture for Human Astroviruses [PDF]

Bio-Protocol, 2020
Human astroviruses (HAstV) are non-enveloped, positive-sense single stranded RNA viruses that typically cause gastroenteritis in children, the elderly and among immunocompromised individuals.
Irene Owusu, Carmen Mirabelli, Abimbola Kolawole   +2 more
doaj   +3 more sources

Utilizing the apical-out enteroids in vitro model to investigate intestinal glucose transport, barrier function, oxidative stress, and inflammatory responses in broiler chickens [PDF]

Frontiers in Physiology
IntroductionConventional 2D intestinal epithelial cell lines have been widely used in investigating intestinal functions, yet with limitations in recapitulating the in vivo gut physiology of chickens.
Peter Mann, Jundi Liu, Liang-en Yu, Ross Wolfenden, Yihang Li   +4 more
doaj   +2 more sources

Differential role of M cells in enteroid infection by Mycobacterium avium subsp. paratuberculosis and Salmonella enterica serovar Typhimurium [PDF]

Frontiers in Cellular and Infection Microbiology
Infection of ruminants such as cattle with Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease, a disease characterized by chronic inflammation of the small intestine and diarrhoea.
Omar A. Alfituri, Rosemary Blake, Kirsty Jensen, Neil A. Mabbott, Jayne Hope, Joanne M. Stevens   +5 more
doaj   +2 more sources

Translocation across a human enteroid monolayer by zoonotic Streptococcus suis correlates with the presence of Gb3-positive cells [PDF]

iScience
Summary: Streptococcus suis is a zoonotic pathogen that can cause meningitis and septicaemia. The consumption of undercooked pig products is an important risk factor for zoonotic infections, suggesting an oral route of infection.
Thomas J. Roodsant, Kees C.H. van der Ark, Constance Schultsz   +2 more
doaj   +2 more sources

Analyzing efficiency of a lentiviral shRNA knockdown system in human enteroids using western blot and flow cytometry. [PDF]

STAR Protoc
Enteroids are in vitro models to study gastrointestinal pathologies and test personalized therapeutics; however, the inherent complexity of enteroids often renders standard gene editing approaches ineffective.
Wilson AP, Moshal KS, Franca AP, Ramani S, Gallucci R, Chaaban H, Burge KY.   +6 more
europepmc   +3 more sources

mSphere of Influence: Organoids and Single-Cell Sequencing, a Powerful Combination To Uncover Epithelial and Immune Cell Interactions in the Human Gut Environment

mSphere, 2020
Jose Lemme-Dumit works in the field of mucosal immunology and vaccines. In this mSphere of Influence article, he reflects on how two papers, “Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett’s epithelium”
Jose M. Lemme-Dumit
doaj   +3 more sources

The role of the minor colonization factor CS14 in adherence to intestinal cell models by geographically diverse ETEC isolates

mSphere, 2023
Enterotoxigenic Escherichia coli (ETEC) is a primary causative agent of diarrhea in travelers and young children in low- to middle-income countries. ETEC adheres to small intestinal epithelia via colonization factors (CFs) and secretes heat-stable toxin ...
Emily M. Smith, Antonia Papadimas, Caitlin Gabor, Ceanna Cooney, Tao Wu, David Rasko, Eileen M. Barry   +6 more
doaj   +1 more source

Piperine as potential therapy of post-weaning porcine diarrheas: an in vitro study using a porcine duodenal enteroid model

BMC Veterinary Research, 2023
Post-weaning diarrhea in piglets is a major problem, resulting in a significant loss in pig production. This study aimed to investigate the effects of piperine, an alkaloid abundantly found in black peppers, on biological activities related to the ...
Saravut Satitsri, Nattaphong Akrimajirachoote, Kanokkan Nunta, Nitwarat Ruennarong, Orawan Amnucksoradej, Chatchai Muanprasat   +5 more
doaj   +1 more source