Results 21 to 30 of about 2,925 (189)

Enteroids to Study Pediatric Intestinal Drug Transport. [PDF]

Mol Pharm
Intestinal maturational changes after birth affect the pharmacokinetics (PK) of drugs, having major implications for drug safety and efficacy. However, little is known about ontogeny-related PK patterns in the intestine. To explore the accuracy of human enteroid monolayers for studying drug transport in the pediatric intestine, we compared the drug ...
Streekstra EJ, Keuper-Navis M, van den Heuvel JJMW, van den Broek P, Stommel MWJ, de Boode W, Botden S, Bervoets S, O'Gorman L, Greupink R, Russel FGM, van de Steeg E, de Wildt SN.   +12 more
europepmc   +6 more sources

Human Breast Milk Enhances Intestinal Mucosal Barrier Function and Innate Immunity in a Healthy Pediatric Human Enteroid Model

Frontiers in Cell and Developmental Biology, 2021
Breastfeeding has been associated with long lasting health benefits. Nutrients and bioactive components of human breast milk promote cell growth, immune development, and shield the infant gut from insults and microbial threats. The molecular and cellular
Gaelle Noel, Julie G. In, Julie G. In, Jose M. Lemme-Dumit, Lauren R. DeVine, Robert N. Cole, Anthony L. Guerrerio, James D. Campbell, Olga Kovbasnjuk, Olga Kovbasnjuk, Marcela F. Pasetti   +10 more
doaj   +1 more source

Human Sapovirus Replication in Human Intestinal Enteroids

Journal of Virology, 2023
Human sapoviruses (HuSaVs) are a frequent but overlooked cause of acute gastroenteritis, especially in children. Little is known about this pathogen, whose successful in vitro cultivation was reported only recently, in a cancer cell-derived line.
Gabriel Euller-Nicolas, Cécile Le Mennec, Julien Schaeffer, Xi-Lei Zeng, Khalil Ettayebi, Robert L. Atmar, Françoise S. Le Guyader, Mary K. Estes, Marion Desdouits   +8 more
openaire   +4 more sources

Organoids to Dissect Gastrointestinal Virus–Host Interactions: What Have We Learned?

Viruses, 2021
Historically, knowledge of human host–enteric pathogen interactions has been elucidated from studies using cancer cells, animal models, clinical data, and occasionally, controlled human infection models. Although much has been learned from these studies,
Sue E. Crawford, Sasirekha Ramani, Sarah E. Blutt, Mary K. Estes   +3 more
doaj   +1 more source

Thymosin β4 dynamics during chicken enteroid development [PDF]

Molecular and Cellular Biochemistry, 2020
AbstractThe sheared avian intestinal villus-crypts exhibit high tendency to self-repair and develop enteroids in culture. Presuming that this transition process involves differential biomolecular changes, we employed matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF–MS) to find whether there were differences in the
Mohan Acharya, Rohana Liyanage, Anamika Gupta, Komala Arsi, Ann M. Donoghue, Jackson O. Lay, Narayan C. Rath   +6 more
openaire   +3 more sources

Iron-tracking strategies: Chaperones capture iron in the cytosolic labile iron pool

Frontiers in Molecular Biosciences, 2023
Cells express hundreds of iron-dependent enzymes that rely on the iron cofactors heme, iron-sulfur clusters, and mono-or di-nuclear iron centers for activity.
Caroline C. Philpott, Olga Protchenko, Yubo Wang, Lorena Novoa-Aponte, Andres Leon-Torres, Samantha Grounds, Amber J. Tietgens   +6 more
doaj   +1 more source

Bovine Enteroids as an In Vitro Model for Infection with Bovine Coronavirus

Viruses, 2023
Bovine coronavirus (BCoV) is one of the major viral pathogens of cattle, responsible for economic losses and causing a substantial impact on animal welfare.
Ruchika Shakya, Alejandro Jiménez-Meléndez, Lucy J. Robertson, Mette Myrmel   +3 more
doaj   +1 more source

The stress polarity signaling (SPS) pathway serves as a marker and a target in the leaky gut barrier: implications in aging and cancer. [PDF]

, 2020
The gut barrier separates trillions of microbes from the largest immune system in the body; when compromised, a "leaky" gut barrier fuels systemic inflammation, which hastens the progression of chronic diseases.
Das, Soumita, Foretz, Marc, Ghosh, Pradipta, Ibeawuchi, Stella-Rita, Lim, Blaze B, Mittal, Yash, Sahoo, Debashis, Sayed, Ibrahim M, Swanson, Lee, Viollet, Benoit   +9 more
core   +3 more sources

Experimental Modeling of Necrotizing Enterocolitis in Human Infant Intestinal Enteroids

Journal of Investigative Surgery, 2022
Background: Experimental model systems are of paramount importance in advancing our understanding of human disease. Methods There are several limitations when using a single cell culture to recapitulate the findings in a complex organism and results ...
Christie Buonpane, Guillermo Ares, Carrie Yuan, Camille Schlegel, Heather Liebe, Catherine J. Hunter   +5 more
doaj   +1 more source

Epithelial and Neutrophil Interactions and Coordinated Response to Shigella in a Human Intestinal Enteroid-Neutrophil Coculture Model

mBio, 2022
Polymorphonuclear neutrophils (PMN) are recruited to the gastrointestinal mucosa in response to inflammation, injury, and infection. Here, we report the development and the characterization of an ex vivo tissue coculture model consisting of human primary
Jose M. Lemme-Dumit, Michele Doucet, Nicholas C. Zachos, Marcela F. Pasetti   +3 more
doaj   +1 more source