Results 31 to 40 of about 43,487 (351)
Enhanced and controlled chromatin extraction from FFPE tissues and the application to ChIP-seq
BMC Genomics, 2019 Background Epigenetic dysregulation is involved in the etiology and progression of various human diseases. Formalin-fixed paraffin-embedded (FFPE) samples represent the gold standard for archiving pathology samples, and thus FFPE samples are a major ...
Jian Zhong +10 more
doaj +1 more source
Single-cell sequencing shows cellular heterogeneity of cutaneous lesions in lupus erythematosus
Nature Communications, 2022 Discoid lupus erythematosus (DLE) and systemic LE (SLE) can present as cutaneous lesions. Here the authors characterise an scRNA dataset of cutaneous lesions from these patients and compare these to healthy controls showing differential immune cell ...
Meiling Zheng +16 more
doaj +1 more source
The SEQC2 epigenomics quality control (EpiQC) study
Genome Biology, 2021 Background Cytosine modifications in DNA such as 5-methylcytosine (5mC) underlie a broad range of developmental processes, maintain cellular lineage specification, and can define or stratify types of cancer and other diseases.
J. Foox +56 more
semanticscholar +1 more source
The Epigenome in Neurodevelopmental Disorders [PDF]
Frontiers in Neuroscience, 2021 Neurodevelopmental diseases (NDDs), such as autism spectrum disorders, epilepsy, and schizophrenia, are characterized by diverse facets of neurological and psychiatric symptoms, differing in etiology, onset and severity. Such symptoms include mental delay, cognitive and language impairments, or restrictions to adaptive and social behavior. Nevertheless,
Julia Reichard +3 more
openaire +5 more sources
Clinical and Translational Medicine, 2022 Background The aberrant differentiation of T follicular helper (Tfh) cells plays an important role in the pathogenesis of systemic lupus erythematosus (SLE). However, the mechanism of regulating Tfh cells differentiation remains unclear.
Limin Liu +11 more
doaj +1 more source
Deep learning-based enhancement of epigenomics data with AtacWorks
Nature Communications, 2021 ATAC-seq is a widely-applied assay used to measure genome-wide chromatin accessibility; however, its ability to detect active regulatory regions can depend on the depth of sequencing coverage and the signal-to-noise ratio.
Avantika Lal +5 more
semanticscholar +1 more source
“Same difference”: comprehensive evaluation of four DNA methylation measurement platforms
Epigenetics & Chromatin, 2018 Background DNA methylation in CpG context is fundamental to the epigenetic regulation of gene expression in higher eukaryotes. Changes in methylation patterns are implicated in many diseases, cellular differentiation, imprinting, and other biological ...
Thadeous J. Kacmarczyk +6 more
doaj +1 more source
The pathological role of B cells in systemic lupus erythematosus: From basic research to clinical
Autoimmunity, 2020 Systemic lupus erythematosus (SLE) is an autoimmune disease that often occurs in females of child-bearing age. It involves multiple systems and severely threatens human life.
Yu Feng +3 more
doaj +1 more source
Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction
Communications Biology, 2021 Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and ...
Christoph Niemöller +13 more
semanticscholar +1 more source
Regenerating the epigenome [PDF]
EMBO reports, 2011 The ability of some organisms to regenerate parts of their body has fascinated scientists for decades. The process of regeneration depends on the potential of certain cells to proliferate and contribute to the formation of new tissue. Organisms have evolved two strategies by which to achieve this: the maintenance of adult stem cells and the induction ...
Juan Carlos Izpisua Belmonte +1 more
openaire +3 more sources