Results 31 to 40 of about 662,881 (280)
Affimer proteins for F-actin: novel affinity reagents that label F-actin in live and fixed cells [PDF]
, 2018 Imaging the actin cytoskeleton in cells uses a wide range of approaches. Typically, a fluorescent derivative of the small cyclic peptide phalloidin is used to image F-actin in fixed cells. Lifeact and F-tractin are popular for imaging the cytoskeleton in
A Nagasaki +36 more
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Biochemistry Research International, 2011 Hsp27 oligomer is reported to interact with F-actin as a barbed-end-capping protein. The present study determined the binding strength and stoichiometry of the interaction using fluorescence of probes attached to Hsp27 cysteine-137.
Philip Graceffa
doaj +1 more source
Nuclear Actin Polymerization Regulates Cell Epithelial‐Mesenchymal Transition
Advanced Science, 2023 Current studies on actin function primarily rely on cytoplasmic actin due to the absence of cellular models specifically expressing nuclear actin. Here, cell models capable of expressing varying levels of nuclear F/G‐actin are generated and a significant
William W. Du +5 more
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Catastrophic disassembly of actin filaments via Mical-mediated oxidation. [PDF]
, 2017 Actin filament assembly and disassembly are vital for cell functions. MICAL Redox enzymes are important post-translational effectors of actin that stereo-specifically oxidize actin's M44 and M47 residues to induce cellular F-actin disassembly.
Ge, Peng +6 more
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F-actin in conifer roots [PDF]
Proceedings of the National Academy of Sciences, 1982 The distribution of F-actin in the complex tissues of a higher plant organ has been visualized by fluorescence labeling the roots of the conifers Chamaecyparis obtusa and Pseudotsuga menziesii with F-actin-specific fluorescent dye-conjugated phallicidin.
T C, Pesacreta +3 more
openaire +2 more sources
Adhesive F-actin waves: a novel integrin-mediated adhesion complex coupled to ventral actin polymerization. [PDF]
PLoS ONE, 2011 At the leading lamellipodium of migrating cells, protrusion of an Arp2/3-nucleated actin network is coupled to formation of integrin-based adhesions, suggesting that Arp2/3-mediated actin polymerization and integrin-dependent adhesion may be ...
Lindsay B Case, Clare M Waterman
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Actin polymerization and depolymerization in developing vertebrates
Frontiers in Physiology, 2023 Development is a complex process that occurs throughout the life cycle. F-actin, a major component of the cytoskeleton, is essential for the morphogenesis of tissues and organs during development.
Yang Bai +14 more
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Roles of Three Domains of Tetrahymena eEF1Ain Bundling F-actin [PDF]
, 2008 The conventional role of eukaryotic elongation factor 1A (eEF1A) is to transport aminoacyl tRNA to the A site of ribosomes during the peptide elongation phase of protein synthesis.
Bunai Fumihide +3 more
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Frontiers in Plant Science, 2017 In vivo markers for F-actin organization and dynamics are extensively used to investigate cellular functions of the actin cytoskeleton, which are essential for plant development and pathogen defense.
Adriana Montes-Rodriguez, Benedikt Kost
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Tracer Studies on F-Actin Fluctuations [PDF]
Physical Review Letters, 2002 We present a study on the fluctuations of semiflexible actin filaments using fluorescence videomicroscopy, focusing on the end-to-end fluctuations of single filaments. In order to specifically measure the position of the polymer's ends, we developed a novel noninvasive method that consists of annealing short end tags to the filaments. This allows us to
Le Goff, Loïc +3 more
openaire +5 more sources