Results 41 to 50 of about 662,881 (280)

A continuous isotropic-nematic liquid crystalline transition of F-actin solutions

, 2002
The phase transition from the isotropic (I) to nematic (N) liquid crystalline suspension of F-actin of average length $3~\mu$m or above was studied by local measurements of optical birefringence and protein concentration. Both parameters were detected to
A. Kerst, A. Suzuki, C. Coppin, F. Gittes, G.L. Vroege, J. Buitenhuis, J. Kas, J.X. Tang, J.X. Tang, Jay X. Tang, Jorge Viamontes, L. Onsager, P.E. Lammert, P.E. Lammert, R. Furukawa, R. Oldenbourg, R.E. Buxbaum, S. Chandrasekhar, S.D. Lee, T. Sato   +19 more
core   +1 more source

F-Actin Dynamics in Neurospora crassa [PDF]

Eukaryotic Cell, 2010
ABSTRACT This study demonstrates the utility of Lifeact for the investigation of actin dynamics in Neurospora crassa and also represents the first report of simultaneous live-cell imaging of the actin and microtubule cytoskeletons in filamentous fungi.
Berepiki, Adokiye, Lichius, Alexander, Shoji, Jun Y., Tilsner, Jens, Read, Nick D.   +4 more
openaire   +4 more sources

Cytoskeletal stabilization of inhibitory interactions in immunologic synapses of mature human dendritic cells with natural killer cells [PDF]

, 2011
Human mature dendritic cells (DCs) can efficiently stimulate natural killer (NK)-cell responses without being targeted by their cytotoxicity. To understand this important regulatory crosstalk, we characterized the development of the immunologic synapse ...
Barreira da Silva, Rosa, Graf, C, Münz, C   +2 more
core   +1 more source

Stabilization of F-Actin Cytoskeleton by Paclitaxel Improves the Blastocyst Developmental Competence through P38 MAPK Activity in Porcine Embryos

Biomedicines, 2022
Changes in F-actin distribution and cortical F-actin morphology are important for blastocyst developmental competence during embryogenesis. However, the effect of paclitaxel as a microtubule stabilizer on embryonic development in pigs remains unclear. We
Seung-Yeon Joe, Seul-Gi Yang, Jae-Ho Lee, Hyo-Jin Park, Deog-Bon Koo   +4 more
doaj   +1 more source

The COOH terminus of the c-Abl tyrosine kinase contains distinct F- and G-actin binding domains with bundling activity [PDF]

, 1994
The myristoylated form of c-Abl protein, as well as the P210bcr/abl protein, have been shown by indirect immunofluorescence to associate with F-actin stress fibers in fibroblasts. Analysis of deletion mutants of c-Abl stably expressed in fibroblasts maps
Baltimore, David, Jackson, Peter K., Janmey, Paul A., Matsudaira, Paul T., Sanders, Mitchell C., Van Etten, Richard A.   +5 more
core   +4 more sources

Vinculin–actin interaction couples actin retrograde flow to focal adhesions, but is dispensable for focal adhesion growth [PDF]

, 2013
In migrating cells, integrin-based focal adhesions (FAs) assemble in protruding lamellipodia in association with rapid filamentous actin (F-actin) assembly and retrograde flow. How dynamic F-actin is coupled to FA is not known.
Alexandrova, Alice Zemljic-Harpf, Bakolitsa, Balaban, Bourdet-Sicard, Brindle, Carisey, Carisey, Chan, Choi, Clare M. Waterman, Cohen, Danuser, Deakin, DeMali, Dumbauld, Galbraith, Gardel, Gaudenz Danuser, Grashoff, Gupton, Hu, Humphries, Ingo Thievessen, Jacob, Jaqaman, Kanchanawong, Karen M. Plevock, Kuo, Lai, Lin, Michael W. Davidson, Mierke, Oakes, Peter M. Thompson, Ponti, Renkawitz, Robert S. Ross, Sabass, Saunders, Schiller, Sergey V. Plotnikov, Sharon L. Campbell, Shemesh, Shen, Shin, Sylvain Berlemont, Turner, Volberg, Wu, Xu, Xu, Zemljic-Harpf, Zhang   +53 more
core   +3 more sources

Pathogenic Germline PALB2 and RAD50 Variants in Patients With Relapsed Ewing Sarcoma

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Approximately 10% of patients with Ewing sarcoma (EwS) have pathogenic germline variants. Here, we report two cases: first, a novel germline pathogenic variant in partner and localizer of BRCA2 (PALB2) in a patient with a late EwS relapse. Its impact on homologous recombination is demonstrated, and breast cancer risk is discussed.
Molly Mack, A. Carolina Tufino, Mohd Azrin Jamalruddin, Sreya Dey, Yinghong Pan, Elina Mukherjee, Jessica D. Daley, Julia Meade, Mahmoud Aarabi, Lisa Maurer, James Cooper, Kara A. Bernstein, Kelly M. Bailey   +12 more
wiley   +1 more source

Induced Arp2/3 Complex Depletion Increases FMNL2/3 Formin Expression and Filopodia Formation

Frontiers in Cell and Developmental Biology, 2021
The Arp2/3 complex generates branched actin filament networks operating in cell edge protrusion and vesicle trafficking. Here we employ a conditional knockout mouse model permitting tissue- or cell-type specific deletion of the murine Actr3 gene ...
Vanessa Dimchev, Vanessa Dimchev, Ines Lahmann, Stefan A. Koestler, Frieda Kage, Frieda Kage, Georgi Dimchev, Georgi Dimchev, Anika Steffen, Theresia E. B. Stradal, Franz Vauti, Hans-Henning Arnold, Klemens Rottner, Klemens Rottner, Klemens Rottner   +14 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Targeting F-actin stress fibers to suppress the dedifferentiated phenotype in chondrocytes

European Journal of Cell Biology
Actin is a central mediator of the chondrocyte phenotype. Monolayer expansion of articular chondrocytes on tissue culture polystyrene, for cell-based repair therapies, leads to chondrocyte dedifferentiation.
Mandy M. Schofield, Alissa T. Rzepski, Stephanie Richardson-Solorzano, Jonah Hammerstedt, Sohan Shah, Chloe E. Mirack, Marin Herrick, Justin Parreno   +7 more
doaj   +1 more source