Results 21 to 30 of about 45,388 (244)

The role of conserved residues in Fdc decarboxylase in prenylated flavin mononucleotide oxidative maturation, cofactor isomerization, and catalysis. [PDF]

J Biol Chem, 2018
The UbiD family of reversible decarboxylases act on aromatic, heteroaromatic, and unsaturated aliphatic acids and utilize a prenylated flavin mononucleotide (prFMN) as cofactor, bound adjacent to a conserved Glu–Arg–Glu/Asp ionic network in the enzyme's ...
Bailey SS, Payne KAP, Fisher K, Marshall SA, Cliff MJ, Spiess R, Parker DA, Rigby SEJ, Leys D.   +8 more
europepmc   +2 more sources

Stereospecificity of hydride transfer and molecular docking in FMN‐dependent NADH‐indigo reductase of Bacillus smithii

FEBS Open Bio, 2021
In this study, we investigated the stereospecificity of hydride transfer from NADH to flavin mononucleotide (FMN) in reactions catalyzed by the FMN‐dependent NADH‐indigo reductase expressed by thermophilic Bacillus smithii.
Kazunari Yoneda, Haruhiko Sakuraba, Tomohiro Araki, Toshihisa Ohshima   +3 more
doaj   +1 more source

Exploring the functional residues in a flavin-binding fluorescent protein using deep mutational scanning. [PDF]

PLoS ONE, 2014
Flavin mononucleotide (FMN)-based fluorescent proteins are versatile reporters that can monitor various cellular processes in both aerobic and anaerobic conditions.
HyeonSeok Shin, Yoobok Cho, Dong-hui Choe, Yujin Jeong, Suhyung Cho, Sun Chang Kim, Byung-Kwan Cho   +6 more
doaj   +1 more source

Riboflavin kinase and pyridoxine 5′-phosphate oxidase complex formation envisages transient interactions for FMN cofactor delivery

Frontiers in Molecular Biosciences, 2023
Enzymes catalysing sequential reactions have developed different mechanisms to control the transport and flux of reactants and intermediates along metabolic pathways, which usually involve direct transfer of metabolites from an enzyme to the next one in ...
Maribel Rivero, Maribel Rivero, Sergio Boneta, Sergio Boneta, Nerea Novo, Nerea Novo, Adrián Velázquez-Campoy, Adrián Velázquez-Campoy, Adrián Velázquez-Campoy, Adrián Velázquez-Campoy, Adrián Velázquez-Campoy, Victor Polo, Victor Polo, Milagros Medina, Milagros Medina, Milagros Medina   +15 more
doaj   +1 more source

Surface-Enhanced Raman Spectroscopic Analysis of Flavoenzyme Cofactors: Guidance for Flavin-Related Bio- and Chemo- Sensors

Chemosensors, 2023
Flavin mononucleotides (FMNs) and flavin adenine nucleotide (FAD) play vital roles in the electron-transfer processes in diverse enzymatic reactions.
Yawen Liu, Hao Ma, Junqi Zhao, Jihong Wang, Xiaoxia Han, Bing Zhao   +5 more
doaj   +1 more source

Production of riboflavin and related cofactors by biotechnological processes

Microbial Cell Factories, 2020
Riboflavin (RF) and its active forms, the cofactors flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), have been extensively used in the food, feed and pharmaceutical industries.
Shuang Liu, Wenya Hu, Zhiwen Wang, Tao Chen   +3 more
doaj   +1 more source

A fluorescence sensor for nitroreductase detection in hypoxic cells and zebrafish

Results in Chemistry, 2021
Nitroreductase (NTR) is a type of cytoplasmic enzyme that depends on flavin mononucleotide or flavin adenine dinucleotide, and that is widely present in tumor cells where it increases during hypoxia. NTR can effectively reduce aromatic nitro compounds to
Wan Sun, Mingqiong Tong, Guofeng Liu, Xiaoyue Wang, Na Fan, Xinfeng Song, Dongying Yang, Di Zhang   +7 more
doaj   +1 more source

The Riboflavin Metabolism in Four Saccharomyces cerevisiae Wine Strains: Assessment in Oenological Condition and Potential Implications with the Light-Struck Taste

Journal of Fungi, 2023
Riboflavin (RF), or vitamin B2, is an essential compound for yeast growth and a precursor of the flavin coenzymes, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), involved in redox and non-redox processes.
Alessandra Di Canito, Alessio Altomare, Daniela Fracassetti, Natalia Messina, Antonio Tirelli, Roberto Foschino, Ileana Vigentini   +6 more
doaj   +1 more source

Requirement of a Functional Flavin Mononucleotide Prenyltransferase for the Activity of a Bacterial Decarboxylase in a Heterologous Muconic Acid Pathway in Saccharomyces cerevisiae. [PDF]

Appl Environ Microbiol, 2017
Weber HE, Gottardi M, Brückner C, Oreb M, Boles E, Tripp J.   +5 more
europepmc   +2 more sources

Enhanced small green fluorescent proteins as a multisensing platform for biosensor development

Frontiers in Bioengineering and Biotechnology, 2022
Engineered light, oxygen, and voltage (LOV)-based proteins are able to fluoresce without oxygen requirement due to the autocatalytic incorporation of exogenous flavin as a chromophore thus allowing for live cell imaging under hypoxic and anaerobic ...
Guo-Teng Liang, Guo-Teng Liang, Guo-Teng Liang, Guo-Teng Liang, Cuixin Lai, Cuixin Lai, Cuixin Lai, Zejun Yue, Zejun Yue, Zejun Yue, Zejun Yue, Hanbin Zhang, Hanbin Zhang, Hanbin Zhang, Danyang Li, Danyang Li, Danyang Li, Zhong Chen, Xingyu Lu, Liang Tao, Liang Tao, Liang Tao, Fedor V. Subach, Kiryl D. Piatkevich, Kiryl D. Piatkevich, Kiryl D. Piatkevich   +25 more
doaj   +1 more source