Results 61 to 70 of about 7,338 (299)

Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1 [PDF]

, 2018
The extracellular electron transfer (EET) mechanism of an isolated Gram-positive Bacillus megaterium strain (LLD-1), identified by 16S rRNA gene sequencing and physiological analysis, was investigated in the present study. The electrochemical activity of
Chen, Bi-Lian, Jiang, Yan-Xia, Yan-Xia Jiang, Li-Dan Liu, You-Fen Dai, Feng Zhao, Le-Xing You, Zhao, Feng, You, Le-Xing, Xiao, Yong, Yong Xiao, Dai, You-Fen, Bi-Lian Chen, Liu, Li-Dan   +13 more
core   +1 more source

Characterization of bacterial fluorescence: insight into rapid detection of bacteria in water

Water Reuse, 2021
Microbial contamination is one of the main risks affecting water safety. Traditional microbial detection methods tend to be time-consuming and labor-intensive.
Yu Mao, Xiao-Wen Chen, Zhuo Chen, Gen-Qiang Chen, Yun Lu, Yin-Hu Wu, Hong-Ying Hu   +6 more
doaj   +1 more source

Exploring the mechnisms of molecular recognition by flavins [PDF]

, 2005
The design and development of chemical models for enzymes depends on the fundamental principles observed in biological systems. Molecular recognition of flavins by various receptors has attracted attention due to their applications as chemical models for
Babu, Cherukuri Raghavendra, Singh, Ram, Geetanjali ,   +2 more
core   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Transcriptional profiling of circulating extracellular vesicles from prebiopsy prostate cancer patients

Molecular Oncology, EarlyView.
RNA profiling of circulating extracellular vesicles (EVs) from blood samples of men undergoing prostate biopsy identifies transcripts associated with clinically significant prostate cancer. Integrative analysis with public tumor datasets links EV‐derived gene signatures to tumor stage and progression‐free survival, highlighting CASP3, XRCC2, and RIT1 ...
Stefan Werner, Pierre Tennstedt, Randi C. Pose, Christian Müller, Katharina Besler, Svenja Schneegans, Malik Alawi, Marie C. Roesch, Sven Peine, Desiree Bonci, Joanna Budna‐Tukan, Evi Lianidou, Catherine Alix‐Panabières, Derya Tilki, Klaus Pantel   +14 more
wiley   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Regioselective Activation of Flavins through Hydrogen-Bonding [PDF]

, 1985
In order to assess the regioselective activation of flavin through hydrogen-bonding, new flavins which can form intramolecular hydrogen-bonding between N(1) and pendent NH3+ or phenolic OH were synthesized. The reactivities of these "N(1)-hydrogen-bonded"
本田, 典昭, 真鍋, 修, 新海, 征治   +2 more
core  

Photo-CIDNP of Solvent-Exposed Flavins in Flavoproteins. [PDF]

J Phys Chem Lett
Photochemically induced dynamic nuclear polarization (photo-CIDNP) is a hyperpolarization NMR technique that enhances the resonances of molecules involved in the formation of spin-correlated radical pairs.
Schmidt A, Schneider H, Illarionov B, Bacher A, Fischer M, Weber S.   +5 more
europepmc   +2 more sources

Anti-flavin antibodies [PDF]

Biochemical Journal, 1987
Antibodies were elicited to FAD by using the hapten N-6-(6-aminohexyl)-FAD conjugated to the immunogenic carrier protein bovine serum albumin. Cross-reactivity was determined by Ouchterlony double-diffusion analysis with N-6-(6-aminohexyl)-FAD coupled to rabbit serum albumin.
M J, Barber, D C, Eichler, L P, Solomonson, B A, Ackrell   +3 more
openaire   +2 more sources

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source