Investigation of possible G-quadruplex formation by GU- and GA-rich repeats and their role in translation [PDF]
RNA BiologyRNA G-quadruplexes (rG4s) are involved in many aspects of cellular and viral protein expression. rG4s consist of at least two stacks of guanine tetrads that are stabilized by non-Watson-Crick-Franklin base pairs.
Bas M. Morren +3 more
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Doublet decoding of tRNASer3 demonstrates plasticity of ribosomal decoding center [PDF]
Nature CommunicationsFrameshifts can be caused by specific combinations of tRNA and mRNA. The wildtype AGC-decoding E. coli tRNASer3 GCU has been shown to induce −1 ribosomal frameshifting on GCA alanine codons, and proposed to read a two-base codon instead of a canonical ...
Shruthi Krishnaswamy +4 more
doaj +2 more sources
Functionality and translation fidelity characterization of mRNA vaccines using platform based mass spectrometry detection [PDF]
npj VaccinesThe success of mRNA-based therapeutics and vaccines is attributed to their rapid development, adaptability, and scalable production. Modified ribonucleotides like N1-methylpseudouridine enhance stability and reduce immunogenicity but were recently found ...
Alyssa Q. Stiving +9 more
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Lycorine Derivative Inhibits SARS-CoV-2 Replication by Reducing -1 Programmed Ribosomal Frameshifting via Targeting ZAP. [PDF]
MedComm (2020)The present study evaluated the antiviral potential of various lycorine derivatives and identified compound 7 as a promising candidate. Compound 7 demonstrated strong antiviral efficacy against both the original SARS‐CoV‐2 and its variants in vitro and in vivo.
Du T +11 more
europepmc +2 more sources
The highly conserved codon following the slippery sequence supports -1 frameshift efficiency at the HIV-1 frameshift site. [PDF]
PLoS ONE, 2015 HIV-1 utilises -1 programmed ribosomal frameshifting to translate structural and enzymatic domains in a defined proportion required for replication. A slippery sequence, U UUU UUA, and a stem-loop are well-defined RNA features modulating -1 frameshifting
Suneeth F Mathew +6 more
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Predicting ribosomal frameshifting efficiency [PDF]
Physical Biology, 2008 Many retroviruses use -1 ribosomal frameshifting as part of the mechanism in translational control of viral protein synthesis. Quantitative prediction of the efficiency of -1 frameshifting is crucial for understanding the viral gene expression. Here we investigate the free energy landscape for a minimal -1 programmed ribosomal frameshifting machinery ...
Song, Cao, Shi-Jie, Chen
openaire +2 more sources
Investigating the correlation between Xrn1-resistant RNAs and frameshifter pseudoknots
RNA Biology, 2023 Xrn1-resistant RNA structures are multifunctional elements employed by an increasing number of RNA viruses. One of such elements is the coremin motif, discovered in plant virus RNAs, of which the structure has been hypothesized to form a yet unelucidated
Ivar W. Dilweg +3 more
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Cell Reports, 2023 Summary: During translation of the genomic RNA of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative virus in the COVID-19 pandemic, host ribosomes undergo programmed ribosomal frameshifting (PRF) at a conserved structural ...
Frederick Rehfeld +5 more
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Antisense-induced ribosomal frameshifting [PDF]
Nucleic Acids Research, 2006 Programmed ribosomal frameshifting provides a mechanism to decode information located in two overlapping reading frames by diverting a proportion of translating ribosomes into a second open reading frame (ORF). The result is the production of two proteins: the product of standard translation from ORF1 and an ORF1-ORF2 fusion protein.
Henderson, Clark M. +2 more
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Regulation of programmed ribosomal frameshifting by co-translational refolding RNA hairpins. [PDF]
PLoS ONE, 2013 RNA structures are unwound for decoding. In the process, they can pause the elongating ribosome for regulation. An example is the stimulation of -1 programmed ribosomal frameshifting, leading to 3' direction slippage of the reading-frame during ...
Che-Pei Cho +4 more
doaj +1 more source