Results 251 to 260 of about 84,275 (305)
Proceedings of the 1st ACM SIGCHI symposium on Engineering interactive computing systems, 2009 In this paper, we describe the design and realization of FRAP, a framework for the construction of pervasive games. With FRAP, we focus on context-aware multi-user chase games that include a strategic component. The game domain is exemplified by the "capture the flag" metaphor.
Jan-Peter Tutzschke, Olaf Zukunft
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Analysis of receptor oligomerization by FRAP microscopy
Nature Methods, 2009 Here we describe an approach to investigate di- or oligomerization of transmembrane receptors in living cells with fluorescence recovery after photobleaching (FRAP). We immobilized a defined fraction of receptors with antibodies and then measured lateral mobility of the nonimmobilized fraction by FRAP.
Dorsch, Sandra +4 more
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FRAP Analysis of Extracellular Diffusion in Zebrafish Embryos
, 2018 Morphogens are signaling molecules that provide positional information to cells during development. They must move through embryonic tissues in order to coordinate patterning. The rate of a morphogen's movement through a tissue-its effective diffusivity-affects the morphogen's distribution and therefore influences patterning.
Soh, Gary H., Müller, Patrick
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Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM [PDF]
Molecules, 2012 Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Hellen C Ishikawa-Ankerhold +1 more
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SMART FRAP: a robust and quantitative FRAP analysis method for phase separation
Chemical Communications, 2023An accurate and robust method to obtain multiplex information by analyzing FRAP data of phase separation condensates.
Xiaotian Wang +4 more
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The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay
Analytical Biochemistry, 1996A simple, automated test measuring the ferric reducing ability of plasma, the FRAP assay, is presented as a novel method for assessing "antioxidant power." Ferric to ferrous ion reduction at low pH causes a colored ferrous-tripyridyltriazine complex to form. FRAP values are obtained by comparing the absorbance change at 593 nm in test reaction mixtures
I F, Benzie, J J, Strain
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Switching Frontal Polymerization Mechanisms: FROMP and FRaP
ACS Macro Letters, 2022Two frontal polymerization (FP) mechanisms, frontal ring-opening metathesis polymerization (FROMP) of dicyclopentadiene and frontal radical polymerization (FRaP) of benzyl acrylate and hexanediol diacrylate, were combined for rapid manufacturing of welded thermoset materials.
Jacob J. Lessard +5 more
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FRAP analysis of binding: proper and fitting
Trends in Cell Biology, 2005Dynamic molecular interactions are fundamental to all cellular processes. In vivo analyses of these interactions are frequently done using fluorescence recovery after photobleaching (FRAP). Proper interpretation of FRAP data yields information about the binding interactions of fluorescently tagged molecules, including the number of binding states and ...
Brian L, Sprague, James G, McNally
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Expanding the scope of quantitative FRAP analysis
Journal of Theoretical Biology, 2010In this study, new mathematical models were developed for analysis of fluorescence recovery after photobleaching (FRAP) data to account for features not represented in previous analysis: conical photobleaching geometry, spatial variations in binding of fluorescent molecules, and directed transport of fluorescent molecules. To facilitate computations in
Mark A, Hallen, Anita T, Layton
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Molecular diffusion and binding analyzed with FRAP
Protoplasma, 2014Intracellular molecular transport and localization are crucial for cells (plant cells as much as mammalian cells) to proliferate and to adapt to diverse environmental conditions. Here, some aspects of the microscopy-based method of fluorescence recovery after photobleaching (FRAP) are introduced.
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