Results 261 to 270 of about 84,275 (305)
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Nucleocytoplasmic shuttling revealed by FRAP and FLIP technologies
Current Opinion in Biotechnology, 2005Protein mobility within cells is of key importance for many cellular functions. Although immunostaining can reveal protein locations in the steady-state, this might not represent the full picture and provides no information about protein movements. Fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP) are two ...
Mario, Köster +2 more
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Analysis of Biomolecular Dynamics by FRAP and Computer Simulation
2014We present a Monte Carlo simulation environment for modelling complex biological molecular interaction networks and for the design, validation, and quantitative analysis of FRAP assays to study these. The program is straightforward in its implementation and can be instructed through an intuitive script language.
Geverts, Bart +2 more
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Once a physicist: Thomas Fraps
Physics World, 2006My interest in science was first sparked by my grandfather, who was an engineer and mathematician. Later I started reading science-fiction and popular-science books by the likes of Carl Sagan and Douglas Hofstadter. I finally decided to study physics when I was doing my military service, which would have been an intellectual vacuum for me had it not ...
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FRAP & FLIP: Two Sides of the Same Coin?
2015The aim of this study is to point out the similarity and differences of data processing based on either FRAP (Fluorescence Recovery After Photobleaching) or FLIP (Fluorescence Loss In Photobleaching) experimental techniques. The core idea, closely related to the sensitivity analysis, is based on discerning between relevant and irrelevant data ...
Stepán Papácek +4 more
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Simulation von FRAP-Experimenten
2015Um die zuvor theoretisch beschriebenen Methoden einem Test zu unterziehen, sollen in diesem Kapitel Simulationen von FRAP-Experimenten durchgefuhrt werden. Die Simulation der Experimente wird mit der Programmiersprache PYTHON durchgefuhrt, speziell werden die PYTHON-Bibliotheken SCIPY[20] und NUMPY[20] fur numerische Berechnungen, Integrationen ...
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Auswertung von FRAP-Experimenten
2015Die Auswertung von FRAP-Experimenten nach AXELROD et al. [3] wird noch heute haufig verwendet, da sie relativ leicht durchzufuhren ist. Im Folgenden werden die Grundzuge der theoretischen Beschreibung eines FRAP-Experiments nach AXELROD et al. dargestellt, dabei wird nur auf seine Uberlegungen zur lateralen Diffusion in planaren Lipidmembranen ...
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Complex Applications of Simple FRAP on Membranes
2009There is an old adage that says “To see is to believe,” and it still seems to be true in many fields of biology. For an experimental validation of hypotheses, modern biology takes advantage of various fluorescence-based techniques (fluorescence microscopy, digital image analysis) for visualization and quantification.
Minchul Kang, Anne K. Kenworthy
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Line-FRAP, A Versatile Method to Measure Diffusion Rates In Vitro and In Vivo
Journal of Molecular Biology, 2021Debabrata Dey +2 more
exaly

