Results 61 to 70 of about 19,439 (257)

Red Alga Porphyridium Supports High‐Yield Production of a Functional Chimeric Hepatitis B Surface Antigen With Strong Cellular and Humoral Immunogenicity

Plant Biotechnology Journal, EarlyView.
ABSTRACT Microalgae represent promising production factories for the light‐driven, cost‐effective production of recombinant proteins. The red microalga Porphyridium purpureum displays particularly favourable transgene expression properties due to the episomal maintenance of transformation vectors at high copy numbers in the nucleus.
Ana‐Maria Pantazica, Alexander Hammel, Iuliana Caras, Irina Ionescu, Catalin Tucureanu, Adrian Onu, Maria Murace, Jihong Liu Clarke, Crina Stavaru, Norica Branza‐Nichita, Ralph Bock   +10 more
wiley   +1 more source

High‐Throughput Screening for Inhibitors of Sialyl‐ and Fucosyltransferases [PDF]

Angewandte Chemie, 2011
Sweet screens: A high-throughput screening platform for identification of inhibitors of sialyl- and fucosyltransferases based on fluorescence polarization (FP) has been developed. An analogue of the natural donor substrate carrying a fluorescent label (green star) is transferred to a glycoprotein acceptor, which results in robust FP.
James C. Paulson, Cory D. Rillahan, Steven J. Brown, Ames C. Register, Hugh Rosen   +4 more
openaire   +3 more sources

GDP-L-fucose : synthesis and role in inflammation [PDF]

, 2007
GDP-L-fucose: synthesis and role in inflammation The migration of leukocytes from intravascular locations to extravascular sites is essential to the immune responses.
Niittymäki, Jaana
core  

CRISPR/Cas9‐Mediated Knockouts of the ALG3 and GNTI in N. benthamiana and Their Application to Pharmaceutical Production

Plant Biotechnology Journal, EarlyView.
ABSTRACT N‐Glycosylation critically influences the efficacy, safety and pharmacokinetic properties of biopharmaceuticals. Plant expression platforms offer multiple advantages for the production of N‐glycosylated proteins, but their use is impeded by the presence of plant‐specific N‐glycan epitopes, which raise concerns of possible immunogenicity to ...
Dolgormaa Bataa, Hiroyuki Kajiura, Reimi Lai Sang Sawada‐Choi, Yukino Yamashita, Takeshi Ishimizu, Ryo Misaki, Atsushi Takeda, Kazuhito Fujiyama   +7 more
wiley   +1 more source

Human fucosyltransferase 6 enables prostate cancer metastasis to bone [PDF]

, 2014
Background: The interaction between human prostate cancer (PCa) cells and bone marrow (BM) endothelium follows a rolling-and-adhesion cascade mediated by E-selectin ligand (ESL): E-selectin.
Barthel, S R, Dimitroff, C J, Guillebon, A D, Hsu, J-w, King, M R, Lee, Y-F, Li, J   +6 more
core   +1 more source

Transcriptional activation of fucosyltransferase (FUT) genes using the CRISPR-dCas9-VPR technology reveals potent N-glycome alterations in colorectal cancer cells

Glycobiology, 2018
Aberrant fucosylation in cancer cells is considered as a signature of malignant cell transformation and it is associated with tumor progression, metastasis and resistance to chemotherapy.
Athanasios Blanas, L. Cornelissen, Maximilianos Kotsias, Joost C. van der Horst, Henri J. van de Vrugt, H. Kalay, D. Spencer, R. P. Kozak, S. V. van Vliet   +8 more
semanticscholar   +1 more source

Heterologous Production of Cyprosin B in Nicotiana benthamiana: Unveiling the Role of the Plant‐Specific Insert Domain in Protein Function and Subcellular Localisation

Plant Biotechnology Journal, EarlyView.
ABSTRACT Plant systems have gained increased attention as an alternative platform for producing heterologous proteins, particularly for industrially relevant proteins. The Cynara cardunculus L. flower extract is traditionally used in cheese production across Mediterranean countries due to its milk‐clotting properties.
Saraladevi Muthusamy, Ramesh Raju Vetukuri, Anneli Lundgren, Sungyong Kim, Pruthvi B. Kalyandurg, Åke Strid, Li‐Hua Zhu, Selvaraju Kanagarajan, Peter E. Brodelius   +8 more
wiley   +1 more source

Carcinoma-associated fucosylated antigens are markers of the epithelial state and can contribute to cell adhesion through CLEC17A (Prolectin) [PDF]

, 2016
International audienceTerminal fucosylated motifs of glycoproteins and glycolipid chains are often altered in cancer cells. We investigated the link between fucosylation changes and critical steps in cancer progression: epithelial-to-mesenchymal ...
Altare, F, Barille-Nion, S, Bernardeau, K, Breiman, A, Drickamer, K, Echasserieau, K, Imberty, A, Le Pendu, J, Robles, MDL, Trecesson, SDC   +9 more
core   +5 more sources

Distinct Molecular Biomechanical Mechanisms Inhibit Endosperm Cell‐Wall Weakening and Seed Germination at Cold and Warm Nonoptimal Temperatures

Plant, Cell &Environment, EarlyView.
ABSTRACT Temperature sensing to adjust developmental rates and phenological responses to different climatic environments is critical for plant survival. Population‐based thermal‐time threshold models predict linear relationships between temperature and, for example, seed germination rates (speed), but the mechanisms are not known.
Tina Steinbrecher, Antje Voegele, Michael Ignatz, Karin Weitbrecht, Safina Khan, Kai Graeber, James E. Hourston, Gerhard Leubner‐Metzger   +7 more
wiley   +1 more source

An essential, kinetoplastid-specific GDP-Fuc: β-D-Gal α-1,2-fucosyltransferase is located in the mitochondrion of Trypanosoma brucei

bioRxiv, 2019
Fucose is a common component of eukaryotic cell-surface glycoconjugates, generally added by Golgi-resident fucosyltransferases. Whereas fucosylated glycoconjugates are rare in kinetoplastids, the biosynthesis of the nucleotide sugar GDP-Fuc has been ...
Giulia Bandini, S. Damerow, M. L. Güther, Hongjie Guo, A. Mehlert, S. Beverley, M. Ferguson   +6 more
semanticscholar   +1 more source