Results 71 to 80 of about 978,977 (295)

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Vaccination with Plasmids Encoding the Fusion Proteins D-S1, D-S1N and O-SN from SARS-CoV-2 Induces an Effective Humoral and Cellular Immune Response in Mice

Vaccines
Background: Next-generation vaccines against coronavirus disease 2019 (COVID-19) focus on inducing a long-lasting immune response against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and its emerging variants.
Noe Juvenal Mendoza-Ramírez, Julio García-Cordero, Gabriela Hernández-Galicia, Nicole Justine Moreno-Licona, Jesus Hernandez, Carlos Cabello-Gutierrez, Joaquín Alejandro Zúñiga-Ramos, Edgar Morales-Rios, Sonia Mayra Pérez-Tapia, Vianney Ortiz-Navarrete, Martha Espinosa-Cantellano, David Andrés Fernández-Benavides, Leticia Cedillo-Barrón   +12 more
doaj   +1 more source

Viruses and viral proteins

IUCrJ, 2014
For more than 30 years X-ray crystallography has been by far the most powerful approach for determining the structures of viruses and viral proteins at atomic resolution.
Nuria Verdaguer, Diego Ferrero, Mathur R. N. Murthy   +2 more
doaj   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

The N‐terminal domains of the paralogous HycE and NuoCD govern assembly of the respective formate hydrogenlyase and NADH dehydrogenase complexes

FEBS Open Bio, 2020
Formate hydrogenlyase (FHL) is the main hydrogen‐producing enzyme complex in enterobacteria. It converts formate to CO2 and H2 via a formate dehydrogenase and a [NiFe]‐hydrogenase.
Philipp Skorupa, Ute Lindenstrauß, Sabrina Burschel, Christian Blumenscheit, Thorsten Friedrich, Constanze Pinske   +5 more
doaj   +1 more source

Gamete Fusion: Key Protein Identified [PDF]

Current Biology, 2008
Is there a common mechanism of eukaryotic sex? Two recent reports highlight an ancient and widely distributed protein that is key to gamete fusion and is a potential target for malaria vaccines.
Goodman, Christopher D., McFadden, Geoffrey I.   +1 more
openaire   +2 more sources

Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]

, 1993
A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Amman, Atkinson, Baekkeskov, Baekkeskov, Berthold, Boitard, Bottazzo, Castano, Dean, Frorath, Goedel, Greenbaum, Harrison, Heike Berg, Hopp, Jochen Seissler, Karjalainen, Kaufman, Kuglin, Kuglin, Ludwig Mauch, Michael Walter, Palmer, Patrick, Seissler, Smith, Smith, Snyder, Su, Wolfgang Northemann   +29 more
core   +1 more source

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Characterization of Annexin V Fusion with the Superfolder GFP in Liposomes Binding and Apoptosis Detection

Frontiers in Physiology, 2017
Programed cell death is a critical and unavoidable part of life. One of the most widely used markers for dying cells, by apoptosis or pyroptosis, is the redistribution of phosphatidylserine (PS) from the inner to the outer plasma membrane leaflet ...
Abdul Qader Abbady, Aya Twair, Aya Twair, Bouthaina Ali, Hossam Murad   +4 more
doaj   +1 more source