Results 81 to 90 of about 10,115 (192)

A novel transgenic reporter of extracellular acidification in zebrafish elucidates skeletal muscle T‐tubule pH regulation

Developmental Dynamics, EarlyView.
Abstract Disruption of extracellular pH and proton‐sensing can profoundly impact cellular and protein functions, leading to developmental defects. To visualize changes in extracellular pH in the developing embryo, we generated a zebrafish transgenic line that ubiquitously expresses the ratiometric pH‐sensitive fluorescent protein pHluorin2, tethered to
Leif R. Neitzel, Maya Silver, Aaron H. Wasserman, Samantha Rea, Charles C. Hong, Charles H. Williams   +5 more
wiley   +1 more source

Nodal and planar cell polarity signaling cooperate to regulate zebrafish convergence and extension gastrulation movements

eLife, 2020
During vertebrate gastrulation, convergence and extension (C and E) of the primary anteroposterior (AP) embryonic axis is driven by polarized mediolateral (ML) cell intercalations and is influenced by AP axial patterning. Nodal signaling is essential for
Margot LK Williams, Lilianna Solnica-Krezel   +1 more
doaj   +1 more source

Intracellular calcium signal at the leading edge regulates mesodermal sheet migration during Xenopus gastrulation

Scientific Reports, 2018
During the gastrulation stage in animal embryogenesis, the cells leading the axial mesoderm migrate toward the anterior side of the embryo, vigorously extending cell protrusions such as lamellipodia.
K. Hayashi, Takamasa S. Yamamoto, N. Ueno   +2 more
semanticscholar   +1 more source

Noncanonical Nucleotides in the Genome Around the Maternal‐Zygotic Transition

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.
In this paper, Kazzazi et al. provide a comprehensive review of the dynamics of nonconventional nucleotides in the genome during early developmental stages, hypothesizing a potential role for these nucleotides in the activation of the zygotic genome. ABSTRACT From the very moment of fertilization and throughout development, the cells of animal embryos ...
Latifa Kazzazy, Dávid Mező, Kinga K. Nagy, Viktória Perey‐Simon, Judit Tóth, Angéla Békési, Beáta Vértessy, Máté Varga   +7 more
wiley   +1 more source

Ins and outs of Spiralian gastrulation

The International Journal of Developmental Biology, 2014
Gastrulation is a critical stage of metazoan development during which endodermal and mesodermal tissues are internalized, and morphogenesis transforms the early embryo into each animal's unique body-plan. While gastrulation has been studied extensively in classic model systems such as flies, worms, and vertebrates, less is known about gastrulation at a
Jonathan Q. Henry, Deirdre C. Lyons
openaire   +4 more sources

CRISPR/Cas9 Knockout of Shell Matrix Protein 1 in the Slipper‐Snail Crepidula atrasolea

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.
Methods for CRISPR/Cas9 shell matrix protein knockout in the snail, Crepidula atrasolea including optimizing RNP delivery and embryo husbandry, to the use of Sanger and MiSeq sequencing, and high‐content screening of embryos to characterize the loss of Shell Matrix Protein 1 (SMP1) during larval shell development.
Grant Batzel, Yiqun Wang, Antonia Bock, Elbereth Chen, Stephanie Neal, Rebecca N. Lopez‐Anido, Yoon Lee, Evan Tjeerdema, Emily Ignatoff, Tejasvi Patil, Gabriela Ramirez, Maryna P. Lesoway, Amro Hamdoun, Deirdre C. Lyons   +13 more
wiley   +1 more source

Embryogenesis in Myrmicine Ants Combines Features of Short Germ‐Band Development With a Progressive Mode of Segmentation

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.
Different insect species exhibit remarkable diversity in the developmental trajectories from fertilized eggs to hatching larvae. For three myrmicine ant species, we observe a novel combination of a developmental features: small embryonic primordia, a mode of embryogenesis termed short/intermediate germ‐band development, and progressive establishment of
Chi‐Chun Fang, Arjuna Rajakumar, Andrew Kenny, Ulrich G. Mueller, Ehab Abouheif, David Stein   +5 more
wiley   +1 more source

Calpain Proteases and the Evolving Signaling Network in Insect Embryonic Patterning

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.
Insect embryonic Dorsal‐Ventral (DV) patterning relies on the BMP and Toll pathways to different extents. Calcium‐dependent cystein proteases of the Calpain family also exert an important function to pattern the DV axis. In Drosophila, Calpain A cleaves the Cactus/IkappaB inhibitor and modifies Toll signals in ventral regions of the embryo. In Rhodnius
Alison Julio, Helena Araujo
wiley   +1 more source

Discrete levels of Twist activity are required to direct distinct cell functions during gastrulation and somatic myogenesis.

PLoS ONE, 2014
Twist (Twi), a conserved basic helix-loop-helix transcriptional regulator, directs the epithelial-to-mesenchymal transition (EMT), and regulates changes in cell fate, cell polarity, cell division and cell migration in organisms from flies to humans ...
Ming-Ching Wong, Krista C Dobi, Mary K Baylies   +2 more
doaj   +1 more source

Stat3/Cdc25a-dependent cell proliferation promotes embryonic axis extension during zebrafish gastrulation

PLoS Genetics, 2017
Cell proliferation has generally been considered dispensable for anteroposterior extension of embryonic axis during vertebrate gastrulation. Signal transducer and activator of transcription 3 (Stat3), a conserved controller of cell proliferation ...
Yinzi Liu, D. Sepich, L. Solnica-Krezel
semanticscholar   +1 more source